CAS 205391-02-2|4,5-Diaminofluorescein diacetate
| Common Name | 4,5-Diaminofluorescein diacetate | ||
|---|---|---|---|
| CAS Number | 205391-02-2 | Molecular Weight | 446.409 |
| Density | 1.5±0.1 g/cm3 | Boiling Point | 719.0±60.0 °C at 760 mmHg |
| Molecular Formula | C24H18N2O7 | Melting Point | / |
| MSDS | ChineseUSA | Flash Point | 320.0±29.2 °C |
Names
| Name | 4,5-Diaminofluorescein diacetate |
|---|---|
| Synonym | More Synonyms |
4,5-Diaminofluorescein diacetate BiologicalActivity
| Description | DAF 2DA is most widely probe for NO measurement. |
|---|---|
| Related Catalog | Signaling Pathways >>Others >>OthersResearch Areas >>OthersDye Reagents |
| In Vitro | Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF 2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF 2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein). |
| References | [1]. Wany A, et al. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence. Methods Mol Biol. 2016;1424:39-47. |
Chemical & Physical Properties
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 719.0±60.0 °C at 760 mmHg |
| Molecular Formula | C24H18N2O7 |
| Molecular Weight | 446.409 |
| Flash Point | 320.0±29.2 °C |
| Exact Mass | 446.111389 |
| PSA | 140.17000 |
| LogP | 1.20 |
| Vapour Pressure | 0.0±2.3 mmHg at 25°C |
| Index of Refraction | 1.721 |
| InChIKey | PTSUYDXEEKDBQU-UHFFFAOYSA-N |
| SMILES | CC(=O)Oc1ccc2c(c1)Oc1cc(OC(C)=O)ccc1C21OC(=O)c2cc(N)c(N)cc21 |
| Storage condition | 2-8℃ |
Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Risk Phrases | R36/38 |
| Safety Phrases | 26 |
| RIDADR | NONH for all modes of transport |
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Synonyms
| 5,6-Diaminofluorescein diacetate |
| Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-bis(acetyloxy)-5,6-diamino- |
| (6'-acetyloxy-5,6-diamino-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl) acetate |
| 5,6-Diamino-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate |
| DAF 2DA |
