CAS 70375-24-5|boc-val-pro-arg-amc hcl

Introduction：Basic information about CAS 70375-24-5|boc-val-pro-arg-amc hcl, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Table of Contents

1. Names
2. boc-val-pro-arg-amc hcl BiologicalActivity
3. Chemical & Physical Properties
4. Safety Information
5. Articles3
6. Synonyms

Common Name	boc-val-pro-arg-amc hcl
CAS Number	70375-24-5	Molecular Weight	664.19300
Density	/	Boiling Point	/
Molecular Formula	C₃₁H₄₆ClN₇O₇	Melting Point	/
MSDS	USA	Flash Point	/

Names

Name	tert-butyl N-[1-[2-[[5-(diaminomethylideneamino)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopentan-2-yl]carbamoyl]pyrrolidin-1-yl]-3-methyl-1-oxobutan-2-yl]carbamate,hydrochloride
Synonym	More Synonyms

boc-val-pro-arg-amc hcl BiologicalActivity

Description	Boc-Val-Pro-Arg-MCA hydrochloride is a sensitive fluorogenic substrate for measuring trypsin-like serine proteases activity[1].
Related Catalog	Research Areas >>Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 胰蛋白酶活性测定[2]1. 将稀释的 Trypsin 酶 (100 μL) 加入到 800 μL 的 50mM Tris-HCl 缓冲液 (pH 8.0) 中。 2. 加入 100 μL 50 μM 底物 Boc-Val-Pro-Arg-MCA 可立即引发反应。 3. 55°C 孵育 10 分钟。 4. 加入 1.5 mL 停止剂 (甲醇:正丁醇:蒸馏水= 35:30:35,v/v/v) 停止反应。 5. 用荧光光谱仪测量荧光强度 (excitation 380 nm, emission 450 nm)。
References	[1]. Liu JY, et al. Purification and characterization of a sarcoplasmic serine proteinase from threadfin bream Nemipterus virgatus muscle. Food Chem. 2019 Jun 30;284:198-204. [2]. Sriket C, et al. Low molecular weight trypsin from hepatopancreas of freshwater prawn (Macrobrachium rosenbergii): Characteristics and biochemical properties. Food Chemistry, 2012, 134(1): 351-358.

Chemical & Physical Properties

Molecular Formula	C₃₁H₄₆ClN₇O₇
Molecular Weight	664.19300
Exact Mass	663.31500
PSA	208.95000
LogP	5.11390
InChIKey	ROQGJTZGLVLVIN-AAJWHBHYSA-N
SMILES	Cc1cc(=O)oc2cc(NC(=O)C(CCCN=C(N)N)NC(=O)C3CCCN3C(=O)C(NC(=O)OC(C)(C)C)C(C)C)ccc12.Cl
Storage condition	−20°C

Safety Information

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR	NONH for all modes of transport
WGK Germany	3

Articles3

TFPI inhibits lectin pathway of complement activation by direct interaction with MASP-2.

Eur. J. Immunol. 45(2) , 544-50, (2015)

The lectin pathway (LP) of complement has a protective function against invading pathogens. Recent studies have also shown that the LP plays an important role in ischemia/reperfusion (I/R)-injury. MBL...

Assay of coagulation proteases using peptide chromogenic and fluorogenic substrates.

Meth. Enzymol. 80 , 341, (1981)

New fluorogenic substrates for alpha-thrombin, factor Xa, kallikreins, and urokinase.

J. Biochem. Tokyo 82 , 1495, (1977)

Twenty peptide-4-methylcoumarin amides (MCA) were newly synthesized and tested as possible substrates for alpha-thrombin, factor Xa, kallikreins, urokinase, and plasmin. These fluorogenic peptides con...

Synonyms

Boc-Val-Pro-Arg-7-amido-4-methylcoumarin hydrochloride

MFCD00058181

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