Introduction：Basic information about CAS 1461-15-0|calcein, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | calcein |
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| CAS Number | 1461-15-0 | Molecular Weight | 622.533 |
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| Density | 1.7±0.1 g/cm3 | Boiling Point | 952.7±65.0 °C at 760 mmHg |
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| Molecular Formula | C30H26N2O13 | Melting Point | 200 °C |
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| MSDS | Chinese | Flash Point | 530.0±34.3 °C |
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Names
| Name | calcein |
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| Synonym | More Synonyms |
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calcein BiologicalActivity
| Description | Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. |
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| Related Catalog | Signaling Pathways >>Others >>OthersDye ReagentsResearch Areas >>Others |
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| In Vitro | Calcein accumulates intracellularly in coelomocytes incubated in coelomic fluid or ISO-EDTA. Coelomocytes incubated in CF show an increase in the calcein fluorescence intensity compared to the control. |
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| Cell Assay | Coelomocytes suspensions (ISO and CF) are separately incubated with calcein-AM (excitation and emission wavelength: 496 nm and 516 nm, respectively) at a final concentration of 200 nM, during 30 min, at 26°C. Calcein-AM is a nonfluorescent ABC transporter substrate whose intracellular accumulation is inversely proportional to ABC transporter activity. Intracellular esterases convert calcein-AM into the fluorescent dye calcein, which is not an ABC transporter substrate, thereby accumulating the dye inside the cell. Therefore, a high fluorescence signal indicates low ABC transporter activity whereas a low fluorescence signal indicates high activity. The fluorescence of samples is measured by flow cytometer. The experiment is repeated six times in duplicates. |
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| References | [1]. Marques-Santos LF, et al. ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas. Fish Shellfish Immunol. 2017 Sep 5;70:195-203. |
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Chemical & Physical Properties
| Density | 1.7±0.1 g/cm3 |
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| Boiling Point | 952.7±65.0 °C at 760 mmHg |
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| Melting Point | 200 °C |
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| Molecular Formula | C30H26N2O13 |
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| Molecular Weight | 622.533 |
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| Flash Point | 530.0±34.3 °C |
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| Exact Mass | 622.143494 |
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| PSA | 231.67000 |
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| LogP | 1.56 |
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| Vapour Pressure | 0.0±0.3 mmHg at 25°C |
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| Index of Refraction | 1.775 |
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| InChIKey | DEGAKNSWVGKMLS-UHFFFAOYSA-N |
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| SMILES | O=C(O)CN(CC(=O)O)Cc1cc2c(cc1O)Oc1cc(O)c(CN(CC(=O)O)CC(=O)O)cc1C21OC(=O)c2ccccc21 |
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| Stability | Stable. Incompatible with strong oxidizing agents. |
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| Water Solubility | insoluble |
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Safety Information
| Hazard Codes | Xn:Harmful; |
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| Risk Phrases | R20/21/22;R36/37/38 |
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| Safety Phrases | S22-S24/25-S36/37/39-S26 |
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| WGK Germany | 3 |
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| HS Code | 32041900 |
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Customs
Synonyms
| Fluorescein-bis(methyliminodiacetic acid) |
| Oftasceinum |
| Fluorexon |
| EINECS 215-957-1 |
| Fluorescein complexon |
| 2-[[7'-[[bis(carboxymethyl)amino]methyl]-3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-2'-yl]methyl-(carboxymethyl)amino]acetic acid |
| 2,2',2'',2'''-[(3',6'-Dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-2',7'-diyl)bis(methylenenitrilo)]tetraacetic acid |
| Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein |
| Oftasceina |
| calcein |
| 2,2',2'',2'''-[(3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-2',7'-diyl)bis(methanediylnitrilo)]tetraacetic acid |
| Oftasceine |
| MFCD00005049 |
| oftasceinum [INN_la] |
