CAS 14541-90-3|Uniblue A sodium salt
| Common Name | Uniblue A sodium salt | ||
|---|---|---|---|
| CAS Number | 14541-90-3 | Molecular Weight | 506.48300 |
| Density | / | Boiling Point | / |
| Molecular Formula | C22H15N2NaO7S2 | Melting Point | >300ºC(lit.) |
| MSDS | ChineseUSA | Flash Point | / |
| Symbol | GHS07 | Signal Word | Warning |
Names
| Name | Uniblue A sodium salt |
|---|---|
| Synonym | More Synonyms |
Uniblue A sodium salt BiologicalActivity
| Description | Uniblue A sodium is a reactive protein stain that can be used in the covalent pre-gel staining of the protein (Ex=594 nM)[1]. |
|---|---|
| Related Catalog | Research Areas >>OthersSignaling Pathways >>Others >>Others |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. Prepare the derivatization buffer: 100 mM NaHCO3, 10% SDS, pH 8-9. 2. Add 10 µL of 200 mM Uniblue A solution (dissolved in derivatization buffer) to 90 µL protein solution. 3. The samples are heated at 100°C for 1 min to perform the staining. 4. Add 100 µL of reducing solution composed of 10% glycerol and 20 mM dithiotreitol (DTT) in 200 mM Tris buffer with pH 6.8. 5. Excess Uniblue A reacts with Tris, resulting in a blue compound, serving as running front indicator in electrophoresis. 6. The sample is heated another minute at 100°C in order to achieve efficient reduction and allowed to cool to room temperature. 7. Add 20 µL alkylation solution containing 550 mM iodoacetamide (IAA), incubated for 5 minutes. 8. the samples are subjected to SDS-PAGE. Note: Dry protein samples or samples in compatible buffers (i.e. free of amines) can be diluted directly with the derivatization buffer solution to 5 mg/mL. In other cases, a prior trichloroacetic acid (TCA)/acetone precipitation or buffer exchange by ultrafiltration is recommended (see below). As positive control, bovine serum albumin (BSA) in derivatization buffer is used at a concentration of 10 mg/mL. |
| References | [1]. Marco A Mata-Gómez, et al. Accelerated identification of proteins by mass spectrometry by employing covalent pre-gel staining with Uniblue A. PLoS One. 2012;7(2):e31438. |
Chemical & Physical Properties
| Melting Point | >300ºC(lit.) |
|---|---|
| Molecular Formula | C22H15N2NaO7S2 |
| Molecular Weight | 506.48300 |
| Exact Mass | 506.02200 |
| PSA | 180.29000 |
| LogP | 5.42490 |
| InChIKey | ANOULJGZTVOIFB-UHFFFAOYSA-M |
| SMILES | C=CS(=O)(=O)c1cccc(Nc2cc(S(=O)(=O)[O-])c(N)c3c2C(=O)c2ccccc2C3=O)c1.[Na+] |
Safety Information
| Symbol | GHS07 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H302 |
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
| Hazard Codes | Xn: Harmful; |
| Risk Phrases | R22 |
| RIDADR | NONH for all modes of transport |
Articles7
More Articles| Lysine-directed staining of proteins for MS-based analyses. Electrophoresis 34(3) , 401-4, (2013) Visualization of proteins and MS-based analyses are elemental tasks in modern biochemistry. Nevertheless, reports about covalent protein dyes and their suitability for subsequent MS experiments remain... | |
| Catalytic decomposition of the reactive dye Uniblue A on hematite. Modeling of the reactive surface. Water Res. 35(3) , 750-60, (2001) Experimental results from the adsorption and subsequent catalytic combustion of the reactive dye Uniblue A on hematite indicate that this iron oxide can be used as an affordable catalyst for environme... | |
| Interaction of anthraquinone dyes with lysozyme: evidences from spectroscopic and docking studies. J. Hazard. Mater. 175(1-3) , 985-91, (2010) The interaction between lysozyme and anthraquinone dyes such as Alizarin Red S, Acid blue 129 and Uniblue was studied using steady state, time resolved fluorescence measurements and docking studies. A... |
Synonyms
| EINECS 238-573-6 |
| Acid Blue 215 |
| UNIBLUE A SODIUM SALT |
| MFCD00010794 |
