Introduction：Basic information about CAS 2150-48-3|PYRONINE B, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
Pyronin B is an organic cationic dye used for the staining of bacteria, mycobacteria and ribonucleic acids. Pyronin B is also used as a small hydrophobic (SH) protein channel inhibitor[1][2].
Related Catalog
Research Areas >>InfectionSignaling Pathways >>Others >>Others
In Vitro
Pyronin B (0-15 μM) inhibits channel activity of approximately 60% of small hydrophobic (SH) proteins at a concentration of 10 μM, and the Kd value of 6.8 μM[1]. Pyronin B (0-0.25 μM) affects RSV replication in Vero cells in a dose-dependent manner, and its TCID 50 (50% tissue culture infection dose) = 0 at the concentration of 0.25 μM[1]. Pyronin B (10 μM) can react with bromate in an oxidation reaction and the reaction rate increases dramatically with the addition of trace amounts of Ti (IV) with the characteristic absorption spectra of 555 nm[2].
References
[1]. Yan Li, et al. Inhibition of the human respiratory syncytial virus small hydrophobic protein and structural variations in a bicelle environment. J Virol. 2014 Oct;88(20):11899-914.
[2]. Zhou Z R, et al. Study of the Catalytic Effects of Trace Titanium on Oxidation of Pyronin B with Hydrogen Peroxide and Its Application[C]//Advanced Materials Research. Trans Tech Publications Ltd, 2013, 602: 1233-1237.
Chemical & Physical Properties
Melting Point
174-176ºC
Molecular Formula
C21H27ClN2O
Molecular Weight
358.905
Exact Mass
358.181183
PSA
19.39000
LogP
1.19980
Appearance of Characters
crystals | Green needles or
InChIKey
CXZRDVVUVDYSCQ-UHFFFAOYSA-M
SMILES
CCN(CC)c1ccc2cc3ccc(=[N+](CC)CC)cc-3oc2c1.[Cl-]
Storage condition
Store at RT.
Water Solubility
Soluble in water, ethyl acetate, methyl acetate, ethylene glycol; slightly soluble in ethanol, methanol, methyl cellosolve
Details of toxic effects not reported other than lethal dose value
MUTATION DATA
TEST SYSTEM :
Insect - Drosophila melanogaster
DOSE/DURATION :
1000 ppm
REFERENCE :
AMNTA4 American Naturalist. (Univ. of Chicago Press, Journals Div., POB 37005, Chicago, IL 60637) V.1- 1867- Volume(issue)/page/year: 87,295,1953 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X3951 No. of Facilities: 32 (estimated) No. of Industries: 1 No. of Occupations: 2 No. of Employees: 162 (estimated) No. of Female Employees: 124 (estimated)
Analysis and testing of biological stains--the Biological Stain Commission Procedures.
Biotech. Histochem. 77(5&6) , 237-275, (2002)
[Fluorescence investigation on interaction between artemisinin (qinghaosu) and hemin and its analytical application].
Guang Pu Xue Yu Guang Pu Fen Xi 26(9) , 1640-3, (2006)
Hemin-catalytic decomposition of artemisinin (qinghaosu, QHS) was studied using pyronine B (PB) as an indicator. The interaction between hemin and QHS was an enzyme-substrate model, and the action sit...
