CAS 856925-71-8|(-)-Blebbistatin
| Common Name | (-)-Blebbistatin | ||
|---|---|---|---|
| CAS Number | 856925-71-8 | Molecular Weight | 292.33 |
| Density | 1.3±0.1 g/cm3 | Boiling Point | 486.7±55.0 °C at 760 mmHg |
| Molecular Formula | C18H16N2O2 | Melting Point | 210-212ºC |
| MSDS | ChineseUSA | Flash Point | 248.1±31.5 °C |
| Symbol | GHS07 | Signal Word | Warning |
Names
| Name | (S)-(-)-Blebbistatin |
|---|---|
| Synonym | More Synonyms |
(-)-Blebbistatin BiologicalActivity
| Description | (-)-Blebbistatin is an S enantiomer of blebbistatin. Blebbistatin is a potent and selective myosin II inhibitor with IC50s ranging from 0.5 to 5 μM. |
|---|---|
| Related Catalog | Signaling Pathways >>Cytoskeleton >>MyosinResearch Areas >>Cardiovascular Disease |
| Target | IC50: 0.5 to 5 μM (myosin II)[1] |
| In Vitro | Blebbistatin potently inhibits several striated muscle myosins as well as vertebrate nonmuscle myosin IIA and IIB with IC50 values ranging from 0.5 to 5 μM. Smooth muscle myosin is only poorly inhibited (IC50=80 μM)[1]. Blebbistatin does not compete with nucleotide binding to the skeletal muscle myosin subfragment-1. The inhibitor preferentially binds to the ATPase intermediate with ADP and phosphate bound at the active site, and it slows down phosphate release. It blocks the myosin heads in a products complex with low actin affinity[2]. In culture-activated hepatic stellate cells, blebbistatin is found to change both cell morphology and function. Stellate cells become smaller, acquire a dendritic morphology and have less myosin IIA-containing stress fibres and vinculin-containing focal adhesions. Blebbistatin impairs silicone wrinkle formation, reduces collagen gel contraction and blocks endothelin-1-induced intracellular Ca2+ release. It promotes wound-induced cell migration[3]. |
| In Vivo | Blebbistatin dose-dependently and completely relax both KCl- and carbachol-induced rat detrusor and endothelin-1-induced human bladder contraction. Pre-incubation with 10 μM blebbistatin attenuates carbachol responsiveness by 65% while blocking electrical field stimulation-induced bladder contraction reaching 50% inhibition at 32 Hz[4]. |
| Cell Assay | Freshly isolated HSCs are replated on 96-well plate. At day 3, medium is replaced by serum-free medium and cells are starved overnight, treated with or without blebbistatin (25 μM) for 2 h followed by stimulation with platelet-derived growth factor-BB (20 ng/mL). After an overnight incubation, the WST-1 cell proliferation assay are performed[3]. |
| References | [1]. Limouze J, et al. Specificity of blebbistatin, an inhibitor of myosin II. J Muscle Res Cell Motil. 2004;25(4-5):337-41. [2]. Kovács M, et al. Mechanism of blebbistatin inhibition of myosin II. J Biol Chem. 2004 Aug 20;279(34):35557-63. [3]. Liu Z, et al. Blebbistatin inhibits contraction and accelerates migration in mouse hepatic stellate cells. Br J Pharmacol. 2010 Jan 1;159(2):304-15. [4]. Zhang X, et al. In vitro and in vivo relaxation of urinary bladder smooth muscle by the selective myosin IIinhibitor, blebbistatin. BJU Int. 2011 Jan;107(2):310-7. |
Chemical & Physical Properties
| Density | 1.3±0.1 g/cm3 |
|---|---|
| Boiling Point | 486.7±55.0 °C at 760 mmHg |
| Melting Point | 210-212ºC |
| Molecular Formula | C18H16N2O2 |
| Molecular Weight | 292.33 |
| Flash Point | 248.1±31.5 °C |
| PSA | 52.90000 |
| LogP | 0.93 |
| Vapour Pressure | 0.0±1.3 mmHg at 25°C |
| Index of Refraction | 1.681 |
| InChIKey | LZAXPYOBKSJSEX-GOSISDBHSA-N |
| SMILES | Cc1ccc2c(c1)C(=O)C1(O)CCN(c3ccccc3)C1=N2 |
| Storage condition | Desiccate at +4°C |
| Water Solubility | DMSO: soluble5mg/mL |
Safety Information
| Symbol | GHS07 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H302-H312-H315-H317-H319-H332-H335 |
| Precautionary Statements | P261-P280-P305 + P351 + P338 |
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Faceshields;Gloves |
| Hazard Codes | Xn |
| Risk Phrases | 20/21/22-36/37/38 |
| Safety Phrases | 26-36/37 |
| RIDADR | NONH for all modes of transport |
Articles12
More Articles| Stretch-stimulated glucose transport in skeletal muscle is regulated by Rac1. J. Physiol. 593(3) , 645-56, (2015) Rac1 regulates stretch-stimulated (i.e. mechanical stress) glucose transport in muscle. Actin depolymerization decreases stretch-induced glucose transport in skeletal muscle. Rac1 is a required part o... | |
| Formation of contractile networks and fibers in the medial cell cortex through myosin-II turnover, contraction, and stress-stabilization. Cytoskeleton (Hoboken.) 72(1) , 29-46, (2015) The morphology of adhered cells depends crucially on the formation of a contractile meshwork of parallel and cross-linked fibers along the contacting surface. The motor activity and minifilament assem... | |
| Intramolecular loop/tail interactions are essential for connexin 43-hemichannel activity. FASEB J. 24 , 4378-95, (2010) Connexin-assembled gap junctions (GJs) and hemichannels coordinate intercellular signaling processes. Although the regulation of connexins in GJs has been well characterized, the molecular determinant... |
Synonyms
| (3aS)-3a-Hydroxy-6-methyl-1-phenyl-1,2,3,3a,4a,8a-hexahydro-4H-pyrrolo[2,3-b]quinolin-4-one |
| 4H-Pyrrolo[2,3-b]quinolin-4-one, 1,2,3,3a,4a,8a-hexahydro-3a-hydroxy-6-methyl-1-phenyl-, (3aS)- |
| (3aS)-3a-hydroxy-6-methyl-1-phenyl-2,3-dihydropyrrolo[2,3-b]quinolin-4-one |
