Introduction：Basic information about CAS 1247825-37-1|USP7/USP47 inhibitor, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | USP7/USP47 inhibitor |
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| CAS Number | 1247825-37-1 | Molecular Weight | 484.39900 |
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| Density | / | Boiling Point | / |
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| Molecular Formula | C18H11Cl2N3O3S3 | Melting Point | / |
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| MSDS | / | Flash Point | / |
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Names
| Name | 4-Cyano-5-[(3,5-dichloro-4-pyridinyl)sulfanyl]-N-[4-(methylsulfon yl)phenyl]-2-thiophenecarboxamide |
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| Synonym | More Synonyms |
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USP7/USP47 inhibitor BiologicalActivity
| Description | USP7/USP47 inhibitor is a selective ubiquitin-specific protease 7/47 (USP7/USP47) inhibitor, with EC50s of 0.42 μM and 1.0 μM, respectively. |
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| Related Catalog | Signaling Pathways >>Cell Cycle/DNA Damage >>DeubiquitinaseResearch Areas >>Cancer |
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| Target | EC50: 0.42 μM (USP7), 1.0 μM (USP47)[1] |
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| In Vitro | USP7/USP47 inhibitor (compound 14) is a selective inhibitor of USP7/USP47 with EC50s of 0.42 μM and 1 μM, respectively. USP7/USP47 inhibitor does not inhibit caspase 3, calpain 1, 20S proteasome, and a panel of representative USPs (USP2, USP5, USP8, USP21, and USP28; EC50 > 31.6 μM). USP7/USP47 inhibitor inhibits the growth of HCT-116 cells with an EC50 of 7.6 μM[1]. |
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| Kinase Assay | The cloning, expression and purification of USP21 from BL21 (DE3) bacteria are performed using standard molecular biology techniques. USP2, USP5, USP7, USP8, USP28, USP47, Ub-PLA2 (Ub-CHOP) and Ub-EKL (Ub-CHOP2) are generated. Caspase 3 and the caspase 3 substrate DEVD-Rh110 are used. Deubiquitylating enzyme, cathepsin B and 20S proteasome chymotrypsin like protease activities are measured. Caspase 3 activity is determined using a similar protocol. Briefly, dose ranges of compound (including USP7/USP47 inhibitor) are incubated with caspase 3 for 30 minutes before the addition of DEVD-Rh110 and reading on a fluorometric plate reader using excitation and emission maxima of 485 nm and 531 nm respectively. The final concentrations of caspase 3 and DEVD-Rh110 are 2 nM and 100 nM respectively[1]. |
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| References | [1]. Weinstock J, et al. Selective Dual Inhibitors of the Cancer-Related Deubiquitylating Proteases USP7 and USP47. ACS Med Chem Lett. 2012 Sep 11;3(10):789-92. |
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Chemical & Physical Properties
| Molecular Formula | C18H11Cl2N3O3S3 |
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| Molecular Weight | 484.39900 |
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| Exact Mass | 482.93400 |
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| PSA | 165.33000 |
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| LogP | 6.59338 |
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| InChIKey | GUDJFFQZIISQJB-UHFFFAOYSA-N |
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| SMILES | CS(=O)(=O)c1ccc(NC(=O)c2cc(C#N)c(Sc3c(Cl)cncc3Cl)s2)cc1 |
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Synonyms
| TERT-BUTYL 4-CYANO-4-PHENYL-1-PIPERIDINECARBOXYLATE |
| 1-BOC-4-CYANO-4-PHENYL-PIPERIDINE |
| 4-cyano-5-((3,5-dichloropyridin-4-yl)thio)-N-(4-(methylsulfonyl)phenyl)thiophene-2-carboxamide |
| N-(tert-butoxycarbonyl)-4-phenyl-4-cyanopiperidine |
| 4-cyano-4-phenyl-piperidine-1-carboxylic acid tert-butyl ester |
| 1-N-BOC-4-PHENYLPIPERIDINE-4-CARBONITRILE |
| USP7-USP47 inhibitor |
| 1-Boc-4-phenyl-4-piperidinecarbonitrile |
| Tert-butyl 4-cyano-4-phenyl-piperidine-1-carboxylate |
| USP7/USP47 inhibitor |
