CAS 4091-99-0|H2DCFDA
Introduction：Basic information about CAS 4091-99-0|H2DCFDA, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | H2DCFDA | ||
|---|---|---|---|
| CAS Number | 4091-99-0 | Molecular Weight | 487.286 |
| Density | 1.5±0.1 g/cm3 | Boiling Point | 593.5±50.0 °C at 760 mmHg |
| Molecular Formula | C24H16Cl2O7 | Melting Point | 209-210ºC(lit.) |
| MSDS | ChineseUSA | Flash Point | 312.7±30.1 °C |
| Symbol | GHS07 | Signal Word | Warning |
Names
| Name | 2-(3,6-diacetyloxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid |
|---|---|
| Synonym | More Synonyms |
H2DCFDA BiologicalActivity
| Description | H2DCFDA is a cell-permeable probe used to detect intracellular reactive oxygen species (ROS). |
|---|---|
| Related Catalog | Signaling Pathways >>Others >>OthersDye ReagentsResearch Areas >>Others |
| In Vitro | Analysis of H2DCFDA probe oxidation by means of flow cytometry technique showed that the basal level of ROS in difESCs is about 6–7 times larger than that in embryonic stem cells (ESCs). Both H2DCFDA and DCFDA probes are non-fluorescent in their initial form but they undergo multistep conversion inside the cell that results in the formation of fluorescent product dichlorofluorescein (DCF). The only difference between these two probes is that the conversion of H2DCFDA involves oxidation. Therefore, fluorescence of the H2DCFDA-treated cells depends on the intracellular ROS level, in contrast to fluorescence associated with the DCFDA probe. Surprisingly, flow cytometry analysis shows that the difference between fluorescence levels of ESCs and difESCs loaded with H2DCFDA is close to the difference between the signals from DCFDA-treated cells. The latter indicates a ROS-independent reason for low fluorescent signal of oxidized H2DCFDA in ESCs[1]. |
| Kinase Assay | H2DCFDA is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use[1]. ROS Measurements[1] For the detection of intracellular ROS level ,ROS-sensitive probe H2DCFDA is used. Adherent cells (ESCs, difESCs, eMSCs, HeLa, U118) are incubated with 5 µM staining solution in PBS in the dark for 30 min at 37°C, then harvested with 0.05% trypsin-EDTA solution, suspended in a fresh medium, and immediately analyzed with flow cytometer. Lymphocytes, both control and PHA-activated, are resuspended in PBS, incubated with 5 µM of H2DCFDA in the dark for 30 min at 37°C, and immediately analyzed. Along with the H2DCFDA probe, if indicated, ROS-insensitive modification of the fluorescein dye DCFDA is used. The staining procedure is the same as for the H2DCFDA[1]. |
| References | [1]. Lyublinskaya OG, et al. Redox environment in stem and differentiated cells: A quantitative approach. Redox Biol. 2017 Aug;12:758-769. |
Chemical & Physical Properties
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 593.5±50.0 °C at 760 mmHg |
| Melting Point | 209-210ºC(lit.) |
| Molecular Formula | C24H16Cl2O7 |
| Molecular Weight | 487.286 |
| Flash Point | 312.7±30.1 °C |
| Exact Mass | 486.027313 |
| PSA | 99.13000 |
| LogP | 4.62 |
| Vapour Pressure | 0.0±1.8 mmHg at 25°C |
| Index of Refraction | 1.635 |
| InChIKey | PXEZTIWVRVSYOK-UHFFFAOYSA-N |
| SMILES | CC(=O)Oc1cc2c(cc1Cl)C(c1ccccc1C(=O)O)c1cc(Cl)c(OC(C)=O)cc1O2 |
| Storage condition | 2-8°C |
Safety Information
| Symbol | GHS07 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H315-H319-H335 |
| Precautionary Statements | P261-P305 + P351 + P338 |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
| Hazard Codes | Xi |
| Risk Phrases | 36/37/38 |
| Safety Phrases | S22-S24/25 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3.0 |
| HS Code | 2932999099 |
Customs
| HS Code | 2932999099 |
|---|---|
| Summary | 2932999099. other heterocyclic compounds with oxygen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |
Articles237
More Articles| Atmospheric-pressure plasma jet induces DNA double-strand breaks that require a Rad51-mediated homologous recombination for repair in Saccharomyces cerevisiae. Arch. Biochem. Biophys. 560 , 1-9, (2014) Non-thermal plasma generated under atmospheric pressure produces a mixture of chemically reactive molecules and has been developed for a number of biomedical applications. Recently, plasma jet has bee... | |
| Heme oxygenase-1 protects corexit 9500A-induced respiratory epithelial injury across species. PLoS ONE 10(4) , e0122275, (2015) The effects of Corexit 9500A (CE) on respiratory epithelial surfaces of terrestrial mammals and marine animals are largely unknown. This study investigated the role of CE-induced heme oxygenase-1 (HO-... | |
| Bilobalide attenuates hypoxia induced oxidative stress, inflammation, and mitochondrial dysfunctions in 3T3-L1 adipocytes via its antioxidant potential. Free Radic. Res. 48(10) , 1206-17, (2014) Excessive expansion of white adipose tissue leads to hypoxia which is considered as a key factor responsible for adipose tissue dysfunction in obesity. Hypoxia induces inflammation, insulin resistance... |
Synonyms
| 2,7-Dichlorodihydrofluorescein diacetate |
| H2DCFDA [2',7'-Dichlorodihydrofluorescein diacetate] |
| MFCD00128955 |
| 2-(3,6-Diacetoxy-2,7-dichloro-9H-xanthen-9-yl)benzoic acid |
| Leucodiacetyldichlorofluorescein |
| H2DCFDA |
| 2',7'-dichlorofluorescin diacetate |
| Benzoic acid, 2-[3,6-bis(acetyloxy)-2,7-dichloro-9H-xanthen-9-yl]- |
| DCFH |
