CAS 150206-05-6|5-(((2,5-DIOXOPYRROLIDIN-1-YL)OXY)CARBONYL)-3-OXO-3H-SPIRO[ISOBENZOFURAN-1,9'-XA
| Common Name | 5-(((2,5-DIOXOPYRROLIDIN-1-YL)OXY)CARBONYL)-3-OXO-3H-SPIRO[ISOBENZOFURAN-1,9'-XANTHENE]-3',6'-DIYL DIACETATE | ||
|---|---|---|---|
| CAS Number | 150206-05-6 | Molecular Weight | 557.461 |
| Density | 1.6±0.1 g/cm3 | Boiling Point | 757.9±70.0 °C at 760 mmHg |
| Molecular Formula | C29H19NO11 | Melting Point | 152-154ºC(lit.) |
| MSDS | USA | Flash Point | 412.2±35.7 °C |
Names
| Name | 5-Carboxy-fluorescein diacetate N-succinimidyl ester |
|---|---|
| Synonym | More Synonyms |
BiologicalActivity
| Description | 5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation[1]. |
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| Related Catalog | Research Areas >>OthersSignaling Pathways >>Others >>Others |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. Dilute the stock solution in PBS (to give a 50 μM solution). 2. Add 110 μL of this solution per milliliter of cells (to give a final concentration of 5 μM) and mix rapidly. 3. After 5 min at room temperature, add 10 vol of PBS containing 5% FBS. 4. Centrifuge cells 5 min at 300 ×g, 20 ℃. 5. Remove the supernatant, and wash threetimes, each time by resuspending in 10 vol PBS containing 5% FBS. 6. Centrifuge cells 5 min at 300 × g, 20 ℃, and removing the supernatant. Note: Labeling occurs rapidly, and it is essential that the tracker is dispersed as evenly and quickly as possible so that cells are uniformly labeled. |
| References | [1]. Christopher R Parish, et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr Protoc Immunol. 2009 Feb;Chapter 4:Unit4.9. |
Chemical & Physical Properties
| Density | 1.6±0.1 g/cm3 |
|---|---|
| Boiling Point | 757.9±70.0 °C at 760 mmHg |
| Melting Point | 152-154ºC(lit.) |
| Molecular Formula | C29H19NO11 |
| Molecular Weight | 557.461 |
| Flash Point | 412.2±35.7 °C |
| Exact Mass | 557.095825 |
| PSA | 151.81000 |
| LogP | 0.50 |
| Vapour Pressure | 0.0±2.6 mmHg at 25°C |
| Index of Refraction | 1.701 |
| InChIKey | SRSXVRUMXPCNAJ-UHFFFAOYSA-N |
| SMILES | CC(=O)Oc1ccc2c(c1)Oc1cc(OC(C)=O)ccc1C21OC(=O)c2cc(C(=O)ON3C(=O)CCC3=O)ccc21 |
Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Hazard Codes | T |
| Risk Phrases | R45 |
| Safety Phrases | S53 |
| RIDADR | NONH for all modes of transport |
Articles1002
More Articles| MHC class II presentation is controlled by the lysosomal small GTPase, Arl8b. J. Immunol. 194(5) , 2079-88, (2015) Dendritic cells (DCs) are specialized APCs with the ability to prime naive T cells. DCs first sample Ags from the environment and then orchestrate their processing and loading onto MHC class II (MHC I... | |
| Coculture of peripheral blood-derived mesenchymal stem cells and endothelial progenitor cells on strontium-doped calcium polyphosphate scaffolds to generate vascularized engineered bone. Tissue Eng. Part A 21(5-6) , 948-59, (2015) Vascularization of engineered bone tissue is critical for ensuring its survival after implantation and it is the primary factor limiting its clinical use. A promising approach is to prevascularize bon... | |
| Olanzapine inhibits proliferation, migration and anchorage-independent growth in human glioblastoma cell lines and enhances temozolomide's antiproliferative effect. J. Neurooncol. 122(1) , 21-33, (2015) The poor prognosis of patients with glioblastoma fuels the search for more effective therapeutic compounds. We previously hypothesised that the neuroleptic olanzapine may enhance antineoplastic effect... |
Synonyms
| Carboxyfluorescein diacetate succinimidyl ester |
| 2,5-Pyrrolidinedione, 1-[[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]oxy]- |
| 5-Carboxyfluorescein diacetate N-Hydroxysuccinimide Ester |
| 5-{[(2,5-Dioxo-1-pyrrolidinyl)oxy]carbonyl}-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate |
| 5-CFDA N-succinimidyl ester |
| (2,5-dioxopyrrolidin-1-yl) 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate |
| 5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester |
