CAS 30931-67-0|ABTS

Introduction：Basic information about CAS 30931-67-0|ABTS, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Table of Contents

1. Names
2. ABTS BiologicalActivity
3. Chemical & Physical Properties
4. Toxicological Information
CHEMICAL IDENTIFICATION
HEALTH HAZARD DATA
ACUTE TOXICITY DATA
MUTATION DATA
5. Safety Information
6. Articles45
7. Synonyms

Common Name	ABTS
CAS Number	30931-67-0	Molecular Weight	548.680
Density	/	Boiling Point	881.2ºC at 760mmHg
Molecular Formula	C₁₈H₂₄N₆O₆S₄	Melting Point	/
MSDS	ChineseUSA	Flash Point	486.7ºC

Names

Name	Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
Synonym	More Synonyms

ABTS BiologicalActivity

Description	ABTS diammonium salt is a substrate for horseradish peroxidase (HRP) conjugate.
Related Catalog	Signaling Pathways >>Others >>OthersBiochemical Assay ReagentsResearch Areas >>Others
In Vitro	A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, as a substrate for HRP conjugate is studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for HRP in terms of sensitivity, ABTS is the most sensitive, stable and the best in visuality by its bluish-green color[1]. ABTS is a typical peroxidase substrate. For purification and characterization peroxidase positive transformants are cultivated in large scale (XL) under conditions that yield active protein in the culture supernatant. After 160 h cultivation an activity of 55,000 U/L in relation to the substrate ABTS is achieved and the supernatant containing the peroxidase is harvested. With ABTS as substrate the peroxidase activity falls significantly when the H2O2 concentration rose above 0.125 mM, indicating that the enzyme is inhibited by H2O2. Maximum reaction rates depending upon substrate tested reache values between 31.2 and 125 µM[2].
Kinase Assay	Enzyme activity is determined photometrically using a temperature controlled multi-mode plate reader or alternatively in a UV/Vis spectrophotometer. Reactions are initiated by addition of the enzyme. Enzyme activity is measured over a period of 10 min at 25°C at the appropriate wavelength for the substrate. One unit (1 U) is defined as the amount of enzyme that converts 1 µmol substrate per minute. Various H2O2 concentrations (0-1250 µM, enzyme concentrations (0.27-54 nM) and substrate concentrations are used to determine the enzyme activity. The activity of rPsaDyP vs ABTS is determined in 100 mM sodium acetate buffer at pH 3.8 and a final H2O2 concentration of 125 µM. Production of the ABTS cation radical is studied at 420 nm (ε420 36,000 L mol-1 cm-1)[2].
References	[1]. Matsuda H, et al. Evaluation of ELISA with ABTS, 2-2'-azino-di-(3-ethylbenzthiazoline sulfonic acid), as the substrate of peroxidase and its application to the diagnosis of schistosomiasis. Jpn J Exp Med. 1984 Jun;54(3):131-8. [2]. Lauber C, et al. Identification, heterologous expression and characterization of a dye-decolorizing peroxidase of Pleurotus sapidus. AMB Express. 2017 Aug 23;7(1):164.

Chemical & Physical Properties

Boiling Point	881.2ºC at 760mmHg
Molecular Formula	C₁₈H₂₄N₆O₆S₄
Molecular Weight	548.680
Flash Point	486.7ºC
Exact Mass	548.064026
PSA	223.04000
LogP	5.47820
InChIKey	OHDRQQURAXLVGJ-UHFFFAOYSA-N
SMILES	CCn1c(=NN=c2sc3cc(S(=O)(=O)[O-])ccc3n2CC)sc2cc(S(=O)(=O)[O-])ccc21.[NH4+].[NH4+]
Water Solubility	H2O: 50 mg/mL, very slightly hazy, green

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: DL7002000

CAS REGISTRY NUMBER :: 30931-67-0

LAST UPDATED :: 199410

DATA ITEMS CITED :: 2

MOLECULAR FORMULA :: C18-H16-N4-O6-S4.2H4-N

MOLECULAR WEIGHT :: 548.72

WISWESSER LINE NOTATION :: T56 BNYSJ B2 GSWQ CUNNU- CT56 BNYSJ B2 GSWQ &ZH 2

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

TYPE OF TEST :: Mutation in microorganisms

TEST SYSTEM :: Bacteria - Klebsiella pneumoniae

DOSE/DURATION :: 11 gm/L

REFERENCE :: JIMMBG Journal of Immunological Methods. (Elsevier Science Pub. Co., Inc., 52 Vanderbilt Ave., New York, NY 10017) V.1- 1971- Volume(issue)/page/year: 36,55,1980

Safety Information

Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xi: Irritant;
Risk Phrases	R36/37/38
Safety Phrases	S26-S36
RIDADR	NONH for all modes of transport
WGK Germany	3
RTECS	DL7002000
HS Code	29342080

Articles45

Differences in the phenolic composition and antioxidant properties between Vitis coignetiae and Vitis vinifera seeds extracts.

Molecules 18(3) , 3410-26, (2013)

Phenolic compounds were extracted from European and Japanese grapevine species (Vitis vinifera and V. coignetiae) seeds using 80% methanol or 80% acetone. The total content of phenolic compounds was d...

Influence of drying and cooking process on the phytochemical content, antioxidant and hypoglycaemic properties of two bell Capsicum annum L. cultivars.

Food Chem. Toxicol. 53 , 392-401, (2013)

The present study evaluates the influence of drying and cooking processes on the health properties of two bell Capsicum annuum L. cultivars Roggiano and Senise compared with fresh peppers. The content...

Inhibition of Oxidative Stress and Skin Aging-Related Enzymes by Prenylated Chalcones and Other Flavonoids from Helichrysum teretifolium.

Molecules 20 , 7143-55, (2015)

Ten flavonoid-related structures viz. heliteretifolin (1), isoxanthohumol (2), 2',4',6'-trihydroxy-3'-prenylchalcone (3), isoglabranin (4), glabranin (5), 7-methoxy-isoglabranin (6), quercetin (7), 4'...

Synonyms

2,2'-Azinobis(3-ethylbenzothiazolin-6-sulfonsaeure)diammoniumsalz

AzBTS-(NH4)2

6-Benzothiazolesulfonate, 2,2'-[(1Z,2Z)-1,2-hydrazinediylidene]bis[3-ethyl-2,3-dihydro-, (2Z,2'Z)-, ammonium salt (1:2)

2,2'-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

ABTS (diammonium salt)

ABTS SUBSTRATE

(2Z,2'Z)-2,2'-(1Z,2Z)-Hydrazine-1,2-diylidenebis(3-ethyl-2,3-dihydro-1,3-benzothiazole-6-sulfonic acid) diammoniate

AzBTS-(NH4)2/ABTS diaMMoniuM salt

diammonium 2,2'-azinobis(3-ethylbenzthiazoline-6-sulphonate)

ABTS ammonium salt

ABTS [=2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt)]

MFCD00010404

2,2'-azinobis-(3-ethyl-2,3-dihydrobenzothiazole-6-sulphonate) diammonium salt

2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt

2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic Acid Ammonium Salt)

ABTS-(NH4)2,PACKAGE WITH 50 TABLETS

ABTS-(NH4)2

2,2'-(1,4-PHENYLENE)BIS-1H-BENZOTHIAZOLE

EINECS 250-396-6

ABTS(R),DIAMMONIUM SALT

Diammonium (2Z,2'Z)-2,2'-[(1Z,2Z)-1,2-hydrazinediylidene]bis(3-ethyl-2,3-dihydro-1,3-benzothiazole-6-sulfonate)

ABTS substrate tablets

ABTS

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