CAS 150347-59-4|5-Carboxyfluorescein diacetate N-succinimidyl ester
| Common Name | 5-Carboxyfluorescein diacetate N-succinimidyl ester | ||
|---|---|---|---|
| CAS Number | 150347-59-4 | Molecular Weight | 557.461 |
| Density | 1.6±0.1 g/cm3 | Boiling Point | 757.9±70.0 °C at 760 mmHg |
| Molecular Formula | C29H19NO11 | Melting Point | 152-154ºC(lit.) |
| MSDS | USA | Flash Point | 412.2±35.7 °C |
Names
| Name | 5(6)-(n-succinimidyloxycarbonyl)-3',6',o,o'-diacetylfluorescein |
|---|---|
| Synonym | More Synonyms |
BiologicalActivity
| Description | CFSE is a fluorescent dye which can track the cell division. |
|---|---|
| Related Catalog | Signaling Pathways >>Others >>OthersDye ReagentsResearch Areas >>Others |
| In Vitro | CFSE is a fluorescent dye which can track the cell division[1]. To examine the ability of immune cells to migrate into live slices, a single-cell suspension of autologous splenocytes or PBMC is isolated, labeling them with CFSE, and adding them to the top of the slices on day 2 of culture. After 6 additional days of culture, the CFSE-labeling immune cells (green) are found to have migrated throughout the slices. No positive cells are found in the slices without the addition of CFSE-labeling immune cells[2]. |
| Cell Assay | Single-cell suspensions of splenocytes (or PBMC) are stained with 1 µM CFSE in PBS for 9 min at 37°C, combined with 20 mL of 10% FBS RPMI-1640 medium at RT for 2 min, centrifuged, washed, and counted. 2×106 CFSE-labeling splenocytes are added to the top of the slices in 24-well membrane culture insert[2]. |
| References | [1]. Cinthia C. Stempin, et al. GRAIL and Otubain-1 are Related to T Cell Hyporesponsiveness during Trypanosoma cruzi Infection. PLoS Negl Trop Dis. 2017 Jan; 11(1): e0005307. [2]. Xiuyun Jiang et al. Long-lived pancreatic ductal adenocarcinoma slice cultures enable precise study of the immune microenvironment. Oncoimmunology. 2017; 6(7): e1333210. |
Chemical & Physical Properties
| Density | 1.6±0.1 g/cm3 |
|---|---|
| Boiling Point | 757.9±70.0 °C at 760 mmHg |
| Melting Point | 152-154ºC(lit.) |
| Molecular Formula | C29H19NO11 |
| Molecular Weight | 557.461 |
| Flash Point | 412.2±35.7 °C |
| Exact Mass | 557.095825 |
| PSA | 151.81000 |
| LogP | 0.50 |
| Vapour Pressure | 0.0±2.6 mmHg at 25°C |
| Index of Refraction | 1.701 |
| Storage condition | -20°C |
Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Hazard Codes | Xi |
| RIDADR | NONH for all modes of transport |
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Synonyms
| 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester |
| Carboxyfluorescein diacetate succinimidyl ester |
| 2,5-Pyrrolidinedione, 1-[[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]oxy]- |
| 5(6)-CFDA (SE [5(6)-Carboxyfluorescein diacetate |
| MFCD00037466 |
| 5-Carboxyfluorescein diacetate N-Hydroxysuccinimide Ester |
| 5-{[(2,5-Dioxo-1-pyrrolidinyl)oxy]carbonyl}-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate |
| 5-CFDA N-succinimidyl ester |
| 5-Carboxyfluorescein diacetate N-succinimidyl ester |
| CFSE |
