Introduction：Basic information about CAS 68542-93-8|Z-Gly-Pro-AMC, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | Z-Gly-Pro-AMC |
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| CAS Number | 68542-93-8 | Molecular Weight | 463.48300 |
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| Density | 1.358g/cm3 | Boiling Point | 770.8ºC at 760 mmHg |
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| Molecular Formula | C25H25N3O6 | Melting Point | 96-102ºC(lit.) |
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| MSDS | / | Flash Point | 420ºC |
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Names
| Name | benzyl N-[2-[(2S)-2-[(4-methyl-2-oxochromen-7-yl)carbamoyl]pyrrolidin-1-yl]-2-oxoethyl]carbamate |
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| Synonym | More Synonyms |
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Z-Gly-Pro-AMC BiologicalActivity
| Description | Z-Gly-Pro-AMC is a fluorogenic substrate. Z-Gly-Pro-AMC is hydrolyzed by prolyl endopeptidase to generate highly fluorescent 7-amido-4-methylcoumarin (HY-D0027). (λex=380 nm, λem=465 nm)[1]. |
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| Related Catalog | Research Areas >>OthersSignaling Pathways >>Others >>Others |
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| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Z-Gly-Pro-AMC assay[1]: 1. Make a 5 μL of plasma sample is pre-incubated with 10 μL of 250 nM FAP inhibitor, 10 μL of 250 nM PREP inhibitor or 10 μL of 0.0025% (v/v) DMSO for 15 min at 37 ℃. 2. Add 35 μL pre-heated Z-Gly-Pro-AMC (380 μM diluted in buffer) to obtain a final concentration of 266 μM. 3. Fluorescence is measured kinetically for 30 min at 37 ℃. |
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| References | [1]. Bracke A, et, al. The development and validation of a combined kinetic fluorometric activity assay for fibroblast activation protein alpha and prolyl oligopeptidase in plasma. Clin Chim Acta. 2019 Aug;495:154-160. |
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Chemical & Physical Properties
| Density | 1.358g/cm3 |
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| Boiling Point | 770.8ºC at 760 mmHg |
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| Melting Point | 96-102ºC(lit.) |
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| Molecular Formula | C25H25N3O6 |
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| Molecular Weight | 463.48300 |
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| Flash Point | 420ºC |
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| Exact Mass | 463.17400 |
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| PSA | 117.95000 |
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| LogP | 3.35910 |
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| Index of Refraction | 1.635 |
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| InChIKey | YWOXKKRKNGWXEG-FQEVSTJZSA-N |
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| SMILES | Cc1cc(=O)oc2cc(NC(=O)C3CCCN3C(=O)CNC(=O)OCc3ccccc3)ccc12 |
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| Storage condition | −20°C |
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Safety Information
| Safety Phrases | S22;S24/25 |
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| WGK Germany | 3 |
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Synonyms
| Z-GP-AMC |
| Z-Gly-Pro-AMC |
| Z-Gly-propmecounh |
| N-Carbobenzoxyglycylprolyl-4-methylcoumarinyl amide |
| Z-Gly-pro-NH-Mec |
| MFCD00038082 |
