CAS 129541-41-9|5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt
| Common Name | 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt | ||
|---|---|---|---|
| CAS Number | 129541-41-9 | Molecular Weight | 521.787 |
| Density | / | Boiling Point | 732.2ºC at 760mmHg |
| Molecular Formula | C20H26BrClN2O7 | Melting Point | 228-230°C |
| MSDS | ChineseUSA | Flash Point | 396.6ºC |
Names
| Name | 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide sodium salt |
|---|---|
| Synonym | More Synonyms |
BiologicalActivity
| Description | X-Gluc sodium is a dye reagent for the detection of β-glucuronidase, an enzyme produced by Escherichia coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract. X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (GUS reporter system)[1]. |
|---|---|
| Related Catalog | Research Areas >>OthersSignaling Pathways >>Others >>Others |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Dissolve 20 mg X-Gluc sodium in 1mL dimethylformamide (DMF) to prepare X-Gluc master mix. 2. Add the prepared X-Gluc solution to agar medium plates at a final concentration of 50 μg/mL without sterilization. 3. Allow the plates to air dry and be used to inoculate the organisms. 4. Incubate the plates at 35°C and observe 16-24 h after inoculation. |
| References | [1]. Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51(5):402-404. |
Chemical & Physical Properties
| Boiling Point | 732.2ºC at 760mmHg |
|---|---|
| Melting Point | 228-230°C |
| Molecular Formula | C20H26BrClN2O7 |
| Molecular Weight | 521.787 |
| Flash Point | 396.6ºC |
| Exact Mass | 520.061157 |
| PSA | 135.07000 |
| Storage condition | −20°C |
Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Hazard Codes | Xi |
| Risk Phrases | R36/37/38 |
| Safety Phrases | S24/25 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| HS Code | 29339990 |
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Synonyms
| 5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Sodium Salt |
| 5-Bromo-4-chloro-3-indolyl β-D-glucuronide cyclohexylammonium salt |
| 5-Bromo-4-chloro-3-indolyl Beta-D-glucuronide cyclohexylammonium salt |
| 5-Bromo-4-chloro-3-indolyl-beta-D-glucuronide cyclohexylammonium salt hydrate |
| BC-Indicator,X-GlcA,X-glucuronide |
| X-gluc/X-GlcA |
| X-Glu,CHA Salt |
| X-Gluc Sodium Salt |
| Cyclohexanamine - 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid (1:1) |
| X-GlcA,X-glucuronide CHA salt |
| 5-Bromo-4-chloro-3-indolyl β-D-Glucuronide Sodium Salt |
| 5-BROMO-4-CHLORO-3-INDOLYLB-D-GLUCURONID E |
| 5-Bromo-4-chloro-3-indolyl β-D-Glucopyranosiduronic Acid Cyclohexylammonium Salt |
| 5-BROMO-4-CHLORO-3-INDOLYL-B-D-GLUCURO-N IDE CYCLOHEXYLA.S. |
| X-Gluc CHA Salt |
| X-GlcA Sodium Salt |
| 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid cyclohexylammonium salt |
| 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide sodium salt |
| 5-BroMo-4-chloro-3-indolyl beta-D-Glucuronide SodiuM Salt |
| Cyclohexanaminium 5-bromo-4-chloro-1H-indol-3-yl β-D-glucopyranosiduronate |
| X-glucuronide CHA salt |
| 5-Bromo-4-chloro-3-indolyl-b-D-glucuronide sodium salt |
| 5-Bromo-4-chlo |
| X-GlcA CHA Salt |
| β-D-Glucopyranosiduronic acid, 5-bromo-4-chloro-1H-indol-3-yl, compd. with cyclohexanamine (1:1) |
| 5-Bromo-4-chloro-3-indolyl-β-D-glucuronide cyclohexylammonium salt |
| MFCD00135782 |
