CAS 900185-02-6|PIK-294
Introduction：Basic information about CAS 900185-02-6|PIK-294, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | PIK-294 | ||
|---|---|---|---|
| CAS Number | 900185-02-6 | Molecular Weight | 489.528 |
| Density | 1.4±0.1 g/cm3 | Boiling Point | 790.4±70.0 °C at 760 mmHg |
| Molecular Formula | C28H23N7O2 | Melting Point | / |
| MSDS | ChineseUSA | Flash Point | 431.8±35.7 °C |
| Symbol | GHS06 | Signal Word | Danger |
Names
| Name | 2-[[4-amino-3-(3-hydroxyphenyl)pyrazolo[3,4-d]pyrimidin-1-yl]methyl]-5-methyl-3-(2-methylphenyl)quinazolin-4-one |
|---|---|
| Synonym | More Synonyms |
PIK-294 BiologicalActivity
| Description | PIK-294 is a potent p110δ-selective inhibitor with an IC50 of 10 nM. |
|---|---|
| Related Catalog | Research Areas >>Cancer |
| Target | p110δ:10 nM (IC50) p110γ:160 nM (IC50) p110β:490 nM (IC50) p110α:10 μM (IC50) |
| In Vitro | Analysis of the specific Class I PI3 Kinase catalytic isoforms p110α (IC50=10 μM), p110β (IC50=0.49 μM), p110δ (IC50=0.01 μM) and p110γ (IC50=0.16 μM) using the inhibitor PIK-294 indicates differential roles in CXCL8-induced neutrophil migration. PIK-294 inhibits both chemokinetic and chemotactic CXCL8-induced migration[1]. When cells are pre-treated with the PI3Kδ selective inhibitor PIK-294, CXCL8-induced migration in the non-gradient and the gradient assay is significantly inhibited. PIK-294 is used at two concentrations 1 μM and 10 μM. Pre-treatment with 1 μM inhibits migration to a greater extent in the non-gradient assay than in the gradient assay. Pre-treatment with 10 μM inhibits migration to a significantly greater extent than the lower dose in both assays. Prior to stimulation with CXCL8, pre-treatment of the cells with the PI3K inhibitors, Wortmannin (50 nM), PIK-294 (10 μM) and AS-605240 (10 μM) for 2 minutes, cause a reduction in the phosphorylation of Akt. Pre-treatment of cells prior to stimulation with GM-CSF and the DMSO control with the PI3K inhibitors Wortmannin (50 nM), PIK-294 (10 μM) and AS-605240 (10 μM) for 2 minutes, reduce the phosphorylation of Akt (p<0.05 for inhibition of PI3Kδ)[2]. |
| Cell Assay | Neutrophils at a concentration of 6×106 cells/mL are pre-treated with 1 μM and 10 μM of the PIK-294 for 30 mins prior to the addition of CXCL8 (100 ng/mL) or 0.5 ng/mL GM-CSF. Then a non-gradient or gradient gel assay depending on the type of migration is performed. The gels are then constructed and the migration studied[2]. |
| References | [1]. Knight ZA, et al. A pharmacological map of the PI3-K family defines a role for p110alpha in insulin signaling. Cell. 2006 May 19;125(4):733-47. [2]. Martin KJ, et al. The role of phosphoinositide 3-kinases in neutrophil migration in 3D collagen gels. PLoS One. 2015 Feb 6;10(2):e0116250. |
Chemical & Physical Properties
| Density | 1.4±0.1 g/cm3 |
|---|---|
| Boiling Point | 790.4±70.0 °C at 760 mmHg |
| Molecular Formula | C28H23N7O2 |
| Molecular Weight | 489.528 |
| Flash Point | 431.8±35.7 °C |
| Exact Mass | 489.191315 |
| PSA | 124.74000 |
| LogP | 3.34 |
| Vapour Pressure | 0.0±2.9 mmHg at 25°C |
| Index of Refraction | 1.753 |
| InChIKey | WFSLJOPRIJSOJR-UHFFFAOYSA-N |
| SMILES | Cc1ccccc1-n1c(Cn2nc(-c3cccc(O)c3)c3c(N)ncnc32)nc2cccc(C)c2c1=O |
| Storage condition | -20℃ |
Safety Information
| Symbol | GHS06 |
|---|---|
| Signal Word | Danger |
| Hazard Statements | H301 |
| Precautionary Statements | P301 + P310 + P330 |
| RIDADR | UN 2811 6.1 / PGIII |
Synonyms
| 2-{[4-Amino-3-(3-hydroxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl]methyl}-5-methyl-3-(2-methylphenyl)-4(3H)-quinazolinone |
| 4(3H)-Quinazolinone, 2-[[4-amino-3-(3-hydroxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl]methyl]-5-methyl-3-(2-methylphenyl)- |
| cc-610 |
| S2227_Selleck |
| 2-((4-amino-3-(3-hydroxyphenyl)-1H-pyrazolo[3,4-d]pyrimidin-1-yl)methyl)-5-methyl-3-o-tolylquinazolin-4(3H)-one |
| PIK 294,PIK294 |
| PIK-294 |
