CAS 70363-83-6|DiSBAC2(5)
| Common Name | DiSBAC2(5) | ||
|---|---|---|---|
| CAS Number | 70363-83-6 | Molecular Weight | 516.63 |
| Density | / | Boiling Point | / |
| Molecular Formula | C27H40N4O6 | Melting Point | / |
| MSDS | USA | Flash Point | / |
Names
| Name | Bis(1,3-dibutylbarbituric acid) trimethine oxonol |
|---|---|
| Synonym | More Synonyms |
DiSBAC2(5) BiologicalActivity
| Description | DiBAC4(3) is a voltage-sensitive fluorescent dye (λex=490 nm, λem=505 nm). |
|---|---|
| Related Catalog | Signaling Pathways >>Others >>OthersDye ReagentsResearch Areas >>Others |
| In Vitro | The membrane hyperpolarization induced by 10 μM Evans blue (EB) in HEKBKα is clearly observed with DiBAC4(3), while the change in membrane potential (MP) by addition of 3 mM tetraethylammonium chloride (TEA) appears more slowly than that measured with microelectrode. The time to peak of hyperpolarization is 2.3±0.9 s (n=4) and 35.0±2.6 s (n=12, P<0.01) by the measurements with microelectrodes and DiBAC4(3), respectively[1]. |
| Cell Assay | Prior to the fluorescence measurements, cells are incubated in KRH (Krebs-Ringer-HEPES) buffer containing with 100 nM DiBAC4(3) for 20 min at room temperature. The stained cells are used for experiments without washing. The fluorescence emission is collected using a 505 nm dicroic mirror and a BA filter (>520 nm)[1]. |
| References | [1]. Yamada A, et al. Usefulness and limitation of DiBAC4(3), a voltage-sensitive fluorescent dye, for the measurement of membrane potentials regulated by recombinant large conductance Ca2+-activated K+ channels in HEK293 cells. Jpn J Pharmacol. 2001 Jul;86(3):342-50. |
Chemical & Physical Properties
| Molecular Formula | C27H40N4O6 |
|---|---|
| Molecular Weight | 516.63 |
| PSA | 200.30000 |
| LogP | 2.90160 |
| Storage condition | 2~8°C |
Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Hazard Codes | Xn |
| RIDADR | NONH for all modes of transport |
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Synonyms
| dibac4(3) |
| MFCD00467633 |
