Introduction：Basic information about CAS 129787-64-0|Rhod-2 AM, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | Rhod-2 AM |
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| CAS Number | 129787-64-0 | Molecular Weight | 1109.92 |
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| Density | / | Boiling Point | / |
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| Molecular Formula | C51H57BrN4O19 | Melting Point | / |
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| MSDS | / | Flash Point | / |
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Names
| Name | rhod-2, am |
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| Synonym | More Synonyms |
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Rhod-2 AM BiologicalActivity
| Description | Rhod-2 AM is a fluorescent, mitochondrial probe (λex=552 nm, λem=581 nm). |
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| Related Catalog | Signaling Pathways >>Others >>OthersDye ReagentsResearch Areas >>Others |
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| In Vitro | A high concentration of Ca2+ in mitochondria is illustrated by punctate labeling in cells, which is consistent with the location of Ca2+ in the mitochondria following fluorescence staining with Rhod-2 AM (Rhod2-AM) at 6 h p.i.. Inhibition of mitochondrial Ca2+ uptake by ruthenium red (RR) or 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) in IMR5 cells infected with poliovirus (PV) is also illustrated following fluorescence staining with Rhod-2 AM at 6 h p.i.[1]. |
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| Cell Assay | For flow cytofluorometry, cells are harvested, pelleted, and resuspended in ice-cold PBS containing 10 mM glucose, 10% fetal bovine serum (FBS), and 10 μM Rhod-2 AM (Rhod2-AM). Mitochondrial calcium levels are determined by the flow cytofluorometry analysis of aliquots of 4×105 cells. For fluorescence microscopy, IMR5 cells are grown on polylysine-coated (10 μg/mL) slides and stained with 7.5 μM Rhod-2 AM in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS for 2 h before poliovirus (PV) infection. Cells are fixed by incubation for 15 min at 4°C in 4% paraformaldehyde. Cells are washed in PBS, and images are acquired with Zeiss Apotome and Axiovision software[1]. |
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| References | [1]. Brisac C, et al. Calcium flux between the endoplasmic reticulum and mitochondrion contributes to poliovirus-induced apoptosis. J Virol. 2010 Dec;84(23):12226-35. |
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Chemical & Physical Properties
| Molecular Formula | C51H57BrN4O19 |
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| Molecular Weight | 1109.92 |
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| PSA | 254.73000 |
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| LogP | 0.94060 |
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Synonyms
| xanthylium |
| 5-tetramethylrhodamine isothiocyanate |
| rhod 2 |
| tetramethylrhodamine-5-isothiocyanate |
| 5-tritc |
| Rhod-2 AM |
