CAS 9002-07-7|Trypsin
| Common Name | Trypsin | ||
|---|---|---|---|
| CAS Number | 9002-07-7 | Molecular Weight | 372.1 |
| Density | / | Boiling Point | / |
| Molecular Formula | C6H15O12P3 | Melting Point | 115°C |
| MSDS | ChineseUSA | Flash Point | / |
| Symbol | GHS07, GHS08 | Signal Word | Danger |
Names
| Name | Trypsin |
|---|---|
| Synonym | More Synonyms |
Trypsin BiologicalActivity
| Description | Trypsin is an enzyme that hydrolyzes proteins at the carboxyl side of the Lysine or Arginine. Trypsin activates PAR2 and PAR4. Trypsin induces cell-to-cell membrane fusion in PDCoV infection by the interaction of S glycoprotein of PDCoV and pAPN. Trypsin also promotes cell proliferation and differentiation. Trypsin can be used in the research of wound healing and neurogenic inflammation[1][2][3][4][6]. |
|---|---|
| Related Catalog | Research Areas >>InfectionSignaling Pathways >>GPCR/G Protein >>Protease-Activated Receptor (PAR)Research Areas >>Inflammation/Immunology |
| Target | PAR2, PAR4[6] |
| In Vitro | Trypsin (5 μg/mL, 24 or 48 h) promotes porcine deltacoronavirus (PDCoV) replication in LLC-PK cells[2]. Trypsin (10 and 50 ng/mL, 12 h) enhances PDCoV cell-to-cell spread in LLC-PK cells by promoting membrane fusion in LLC-PK cells[2]. Trypsin (0.05%, 3 h) promotes C6 glioma cell proliferation in serum-free and growth factor-free medium[3]. Trypsin (20 -150 ng/mL, 5 days) potentiates PBMC differentiation[4]. Western Blot Analysis[2] Cell Line: LLC-PK cells, ST cells Concentration: 5 μg/mL Incubation Time: 24 or 48 h Result: Promoted PDCoV replication in LLC-PK cells but not ST cells. Immunofluorescence[2] Cell Line: LLC-PK cells, ST cells Concentration: 10 and 50 ng/mL Incubation Time: 12 h Result: Significantly increased cell-to-cell fusion activity during PDCoV infection of LLC-PK cells. |
| In Vivo | Trypsin (100-500 μg per site in 50 μL saline, intradermal injection) induces scratching behaviour in mice[5]. Animal Model: Swiss mice[5] Dosage: 100-500 μg per site, in saline (50 μL) Administration: Intradermal injection Result: Induced pruritus, and was inhibited by trypsin inhibitor. |
| References | [1]. Bhupendra S.Kaphalia. Chapter 16 - Biomarkers of acute and chronic pancreatitis. Biomarkers in Toxicology. 2014, Pages 279-289. [2]. Yue-Lin Yang,et al. Trypsin promotes porcine deltacoronavirus mediating cell-to-cell fusion in a cell type-dependent manner. Emerg Microbes Infect. 2020 Feb 24;9(1):457-468. [3]. H Amano, et al. Trypsin promotes C6 glioma cell proliferation in serum- and growth factor-free medium. Neurosci Res. 1996 Jul;25(3):203-8. [4]. Michael J. V. White, et al. Trypsin Potentiates Human Fibrocyte Differentiation. PLoS One. 2013; 8(8): e70795. [5]. R Costa, et al. Evidence for the role of neurogenic inflammation components in trypsin-elicited scratching behaviour in mice. Br J Pharmacol. 2008 Jul;154(5):1094-103. [6]. F Schmidlin, et al. Protease-activated receptors: how proteases signal to cells. Curr Opin Pharmacol. 2001 Dec;1(6):575-82. |
Chemical & Physical Properties
| Melting Point | 115°C |
|---|---|
| Molecular Formula | C6H15O12P3 |
| Molecular Weight | 372.1 |
| Appearance of Characters | lyophilized powder |
| Storage condition | −20°C |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| Water Solubility | Reconstitute in aqueous buffer | Soluble in water (10 mg/ml), phosphate buffers (10 mg/ml), and balanced salt solutions (1 mg/ml). |
Safety Information
| Symbol | GHS07, GHS08 |
|---|---|
| Signal Word | Danger |
| Hazard Statements | H315-H319-H334-H335 |
| Precautionary Statements | P261-P305 + P351 + P338-P342 + P311 |
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Faceshields;Gloves |
| Hazard Codes | Xn,B |
| Risk Phrases | 36/37/38-42-42/43 |
| Safety Phrases | 22-24-26-36/37-45-23 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 2 |
| RTECS | GC3050000 |
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Synonyms
| MFCD00082094 |
| EINECS 232-650-8 |
