CAS 12627-53-1|LEISHMAN'S STAIN

Introduction:Basic information about CAS 12627-53-1|LEISHMAN'S STAIN, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
Common NameLEISHMAN'S STAIN
CAS Number12627-53-1Molecular Weight/
Density/Boiling Point/
Molecular Formulan.a.Melting Point/
MSDSChineseUSAFlash Point/

Names

NameLeishman Stain
SynonymMore Synonyms

LEISHMAN'S STAIN BiologicalActivity

DescriptionLeishman's stain is an essential staining tool for for staining of the peripheral blood and bone marrow smears (displayed pale bluish-grey to deep blue under oil-immersion lens)[1].
Related CatalogResearch Areas >>OthersSignaling Pathways >>Others >>Others
In VitroGuidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. Prepare stock solution of Sorensen’s buffer: Solution A. Na2HPO4•2H2O (9.5 g/L); Solution B. KH2PO4 (9.1 g/L). 2. Cover the smear with Leishman stain for 2 min. 3. Add double volume of Sorensen buffer (6.8 pH) to the slide and mix gently for 15 min. 4. Wash off with running water and dry the smear (5 min).
References

[1]. S Gajendra, et al. Leishman and Giemsa stain: a new reliable staining technique for blood/bone marrow smears. Int J Lab Hematol. 2015 Dec;37(6):774-82.

Chemical & Physical Properties

Molecular Formulan.a.
Appearance of CharactersPowder
Storage conditionStore at +15°C to +30°C.
Water Solubilitymethanol: soluble1mg/mL | Soluble in water (4 mg/ml), methanol (1 mg/ml), ethanol (4 mg/ml), and methyl cellosolve (20 mg/ml).

Safety Information

Personal Protective EquipmentEyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard CodesXi
Risk Phrases36/38-36/37/38
Safety PhrasesS22;S24/25
RIDADRNONH for all modes of transport
WGK Germany3

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Synonyms

EINECS 235-732-1
MFCD00131498
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