CAS 878674-84-1|BODIPY 505/515-8-C3-COOH

Introduction：Basic information about CAS 878674-84-1|BODIPY 505/515-8-C3-COOH, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
Common NameBODIPY 505/515-8-C3-COOH
CAS Number878674-84-1Molecular Weight335.1765864
Density/Boiling Point/
Molecular FormulaC17H22BF2N2O2Melting Point/
MSDS/Flash Point/

Names

Name4-(4,4-Difluoro-1,3,5,7-tetraMethyl-4-bora-3a,4a-diaza-s-indacene-8-yl)-butyric Acid

BODIPY 505/515-8-C3-COOH BiologicalActivity

DescriptionBODIPY 505/515-8-C3-COOH is a green fluorescing derivative, as a fluorescent dye for imaging lipid droplets in nannochloropsis. BODIPY 505/515-8-C3-COOH can be used for the research of flow cytometric high-throughput screening and cell sorting[1].
Related CatalogResearch Areas >>OthersSignaling Pathways >>Others >>Others
In VitroGuidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Labeling of Cells: 1. Fresh N. oceanica cultures are diluted to ~4×106 cells/ml with ASW and kept at 22℃ prior to any treatment. Incubate the cells according to your normal protocol. 2. BODIPY 505/515 is dissolved in DMSO at 4 mg/ml and diluted with DMSO to different working stock concentrations. 3. Cell suspensions are supplemented with the appropriate BODIPY 505/515 working stock to a specific DMSO concentration between 2 and 10% (v/v) with final BODIPY concentrations between 0.8 and 4 μg/ml. 4. Pure DMSO was used for control treatments. 1 ml of fresh culture was diluted to ~4×106 cells/ml with ASW and stained with 6% DMSO and 1.2 μg/ml BODIPY for 15 min (non-stressed cultures) or with 10% DMSO and 1.6 μg/ml BODIPY for 36 min (stressed cultures). 5. Upon addition of the dye, samples were vortexed for 5 s and then incubated in the dark for 15 min before flow cytometric analysis, if not indicated otherwise.
References

[1]. ChristianSüdfeld, et al. Optimization of high-throughput lipid screening of the microalga Nannochloropsis oceanica using BODIPY 505/515. Algal Research,

Chemical & Physical Properties

Molecular FormulaC17H22BF2N2O2
Molecular Weight335.1765864
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