2,3,5-Triphenyltetrazolium chloride CAS 298-96-4
Introduction：Basic information about 2,3,5-Triphenyltetrazolium chloride CAS 298-96-4, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
2,3,5-Triphenyltetrazolium chloride Basic information
| Product Name: | 2,3,5-Triphenyltetrazolium chloride |
| Synonyms: | 2,3,5-TriphenyltetrazoliuM chlorideSynonyMs 2,3,5-Triphenyl-2H-tetrazoliuM chloride;TTC,TTZ,Tetrazolium Red;FLJ37239;LGMD2K;10 MG ABTS TABLETS;BCIP P-TOLUIDINE SALT ULTRA PURE GRADE;TSA PENASE CONTACT 55MM TRIPLE-WRAP;red tetazolium |
| CAS: | 298-96-4 |
| MF: | C19H15ClN4 |
| MW: | 334.8 |
| EINECS: | 206-071-6 |
| Product Categories: | substrate;Tetrazolium Salts;Tetrazolium Salts & Formazans;Tetrazolium salt |
| Mol File: | 298-96-4.mol |
2,3,5-Triphenyltetrazolium chloride Chemical Properties
| Melting point | 250 °C(lit.) |
| Boiling point | 490.96°C (rough estimate) |
| density | 1.1704 (rough estimate) |
| bulk density | 230kg/m3 |
| refractive index | 1.6110 (estimate) |
| storage temp. | 2-8°C |
| solubility | H2O: may be clear to slightly hazy |
| form | powder |
| color | faint yellow |
| PH | 3.7 (20°C, 10g/L in H2O) |
| Water Solubility | Soluble in water, ethanol and acetone. Insoluble in ether. |
| λmax | 247 nm |
| Sensitive | Light Sensitive & Hygroscopic |
| Merck | 14,9744 |
| BRN | 3923356 |
| Stability: | Stable. Light sensitive. Protect from moisture. Incompatible with strong oxidizing agents. |
| Biological Applications | Algae viability assay; microbial growth assays; transketolase activity screening assays; detecting bacteria,g-hydroxybutyric acid (GHB),microorganisms,myocardial infarction; measuring dehydrogenase activity; treating cancer |
| Major Application | diagnostic assay manufacturing hematology histology |
| InChI | 1S/C19H15N4.ClH/c1-4-10-16(11-5-1)19-20-22(17-12-6-2-7-13-17)23(21-19)18-14-8-3-9-15-18;/h1-15H;1H/q+1;/p-1 |
| InChIKey | PKDBCJSWQUOKDO-UHFFFAOYSA-M |
| SMILES | [Cl-].c1ccc(cc1)-c2nn(-c3ccccc3)[n+](n2)-c4ccccc4 |
| CAS DataBase Reference | 298-96-4(CAS DataBase Reference) |
| EPA Substance Registry System | 2,3,5-Triphenyltetrazolium chloride (298-96-4) |
Safety Information
| Hazard Codes | Xi,Xn |
| Risk Statements | 36/37/38-20/21/22 |
| Safety Statements | 26-36 |
| WGK Germany | 3 |
| RTECS | XF8100000 |
| F | 3-8-10 |
| TSCA | TSCA listed |
| HS Code | 29339900 |
| Storage Class | 4.1B - Flammable solid hazardous materials |
| Hazard Classifications | Eye Irrit. 2 Flam. Sol. 1 Skin Irrit. 2 |
| Toxicity | LD50 intravenous in mouse: 5600ug/kg |
| Chemical Properties | white powder |
| Uses | 2,3,5-Triphenyl-2H-tetrazolium chloride is used as a dye in cell biological studies. |
| Uses | 2,3,5-Triphenyltetrazolium chloride is a selective serotonin 5-HT4 receptor agonist and 5-HT3 gastrointestinal prokinetic agent for functional dyspepsia |
| Uses | In analytical chemistry as a sensitive reagent for reducing sugars, and to distinguish between a-ketols and simple aldehydes. For staining plant and animal tissue. Germination indicator in testing the ability of seeds to germinate: G. Lakon, Ber. Dtsch. Bot. Ges. 60, 299, 434 (1942). In histochemical studies. In determination of antibiotics; of dehydrogenases. |
| Definition | ChEBI: An organic chloride salt having 2,3,5-triphenyltetrazolium as the counterion. |
| General Description | 2,3,5-Triphenyltetrazolium chloride is a colorless water-soluble dye. In the mitochondria of living cells, it is reduced to a deep red, water-insoluble compound (formazan). 2,3,5-Triphenyltetrazolium chloride helps to distinguish between viable and infarcted brain tissue after stroke. |
| Synthesis | 531-52-2 298-96-4 E. The 2,3,5-triphenylformamidine (IV-1) solid obtained in Step D was mixed with 300 mL of dichloromethane and 300 mL of water. After the solid was completely dissolved, N-chlorosuccinimide (0.11 mol, 14.7 g) was added and the reaction was carried out with stirring at 0 °C for 5 h. F. The reaction mixture from Step E was allowed to partition and the upper aqueous phase was separated. The organic phase was distilled under reduced pressure to remove the solvent to give the crude product 2,3,5-triphenyltetrazole (V-1). The crude product was purified by recrystallization with chloroform to give 30.2 g of pure product (yield: 90%). |
| Purification Methods | Crystallise TTZ from EtOH or CHCl3, and dry it at 105o. [Beilstein 26 H 363, 26 II 216, 26 III/IV 1774.] |
| References | [1] Indian Journal of Chemistry, Section B: Organic Chemistry Including Medicinal Chemistry, 1983, vol. 22, # 8, p. 771 - 775 [2] J. Appl. Chem. USSR (Engl. Transl.), 1983, vol. 56, # 3, p. 617 - 621 [3] Zhurnal Prikladnoi Khimii (Sankt-Peterburg, Russian Federation), 1983, vol. 56, # 3, p. 649 - 653 [4] Patent: CN108440433, 2018, A. Location in patent: Paragraph 0057; 0058; 0059; 0064; 0065 [5] Indian Journal of Chemistry, Section B: Organic Chemistry Including Medicinal Chemistry, 1981, vol. 20, # 5, p. 404 - 406 |
2,3,5-Triphenyltetrazolium chloride Preparation Products And Raw materials
| Raw materials | Sodium hydroxide-->Hydrochloric acid-->Acetic acid-->Sodium nitrite-->Sodium acetate-->Aniline-->Sodium hypochlorite-->Benzaldehyde-->Phenylhydrazine-->Triphenylformazan |
