Introduction：Basic information about L-Lactic dehydrogenase CAS 9001-60-9, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
L-Lactic dehydrogenase Basic information
| Product Name: | L-Lactic dehydrogenase |
| Synonyms: | IUB: 1.1.1.27;LACTIC DEHYDROGENASE;LACTIC DEHYDROGENASE BOVINE HEART;LACTIC ACID DEHYDROGENASE;LACTATE DEHYDROGENASE;LACTATE DEHYDROGENASE, HUMAN MUSCLE;LACTATE DEHYDROGENASE ISOENZYME-1, HUMAN;L-LACTATE DEHYDROGENASE |
| CAS: | 9001-60-9 |
| MF: | NULL |
| MW: | 0 |
| EINECS: | 232-617-8 |
| Product Categories: | 1.1.x.x Acting on hydroxyl groups;1.x.x.x Oxidoreductases;Enzyme Class Index;enzyme;Oxidoreductases;Specialty Enzymes |
| Mol File: | Mol File |
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L-Lactic dehydrogenase Chemical Properties
| storage temp. | 2-8°C |
| solubility | aqueous buffer: soluble |
| form | suspension |
| color | white |
| PH | 7.5 |
| biological source | rabbit muscle |
| Water Solubility | low ionic strength buffer: soluble
water: soluble |
| Merck | 13,5349 |
| Specific Activity | 800-1,200units/mg protein |
| Cosmetics Ingredients Functions | SKIN CONDITIONING |
| CAS DataBase Reference | 9001-60-9 |
| EPA Substance Registry System | Dehydrogenase, lactate (9001-60-9) |
Safety Information
| Risk Statements | 21/22-36/37/38 |
| Safety Statements | 22-24/25-36/37-26 |
| WGK Germany | 3 |
| F | 3-10 |
| TSCA | TSCA listed |
| HS Code | 35079090 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Resp. Sens. 1 |
L-Lactic dehydrogenase Usage And Synthesis
| Chemical Properties | Off-white lyophilized powder or crystalline suspension of 3.2 mol/L ammonium sulfate, soluble in water, pI of 4.6, optimum pH 6.0.Stability: crystalline enzyme solution can be stored for 6-8 weeks with unchanged activity, sulfate suspension can be stabilized for more than a year, very dilute solution is unstable; optimum temperature of 39 ℃, rapid inactivation of temperatures above 60 ℃. Inhibitors are excess pyruvate, excess NAD, oxalic acid, oxalic acid monoamide, hydroxymalonic acid, malonic acid, urea, Cu2+, Hg2+, Ag+, 4-chloromercuric benzoic acid, pyrophosphoric acid or phosphate buffer and non-competitive inhibitors formed from coenzyme I (NAD) in alkaline inhibit oxidation reaction, excess lactate and non-competitive inhibitors formed from reduced coenzyme I (NADH) inhibit reduction reaction. inhibitors inhibit the reduction reaction; activators are 2-amino-2-methyl-1-propanol, fluoride, diethanolamine, and heparin (oxidizing). Enzymatic reaction: pyruvate + reduced coenzyme I + H+ ═ lactate + coenzyme I. |
| Uses | L-Lactic Dehydrogenase from bovine heart has been used in ATPase assay of R2 complex Rvb1p-Rvb2p, RecA protein and sarcoplasmic reticulum Ca2+-ATPase (SERCA). |
| Uses | L-Lactic dehydrogenase is used in the determination of pyruvate (used in conjunction with reduced coenzyme) and in the diagnosis of myocardial infarction and leukemia. |
| Uses | L-Lactic Dehydrogenase from bovine heart has been used as a standard in cytotoxicity assay. It has also been used in glutamic pyruvic transaminase (GPT) assay. |
| General Description | L-lactate dehydrogenase (LDH) is an enzyme that catalyzes the conversion of lactate to pyruvate.?In particular, lactic dehydrogenase A (LDHA) is mainly found in skeletal muscle, and for that reason is known as the M subunit. This recombinant form of LDHA has a C-terminal histidine-tag.
The gene LDHA (L-lactate dehydrogenase A chain) is mapped to human chromosome 11p15. It is a subunit of lactate dehydrogenase. |
| Biochem/physiol Actions | Also catalyzes the oxidation of other L-2-hydroxymonocarboxylic acids. |
| Purification Methods | A forty-fold purification of the dehydrogenase is effected by affinity chromatography using Sepharose 4B coupled to 8-(6-aminohexyl)amino-5'-AMP or -NAD . [Lees et al. Arch Biochem Biophys 163 561 1974, Pesce et al. J Biol Chem 239 1753 1964.] |
L-Lactic dehydrogenase Preparation Products And Raw materials
| Raw materials | Calcium phosphate |
