Introduction：Basic information about CAS 41365-32-6|Cirsilineol, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
Cirsilineol, a natural flavone compound, selectively inhibits IFN-γ/STAT1/T-bet signaling in intestinal CD4+ T cells. Cirsilineol has potent immunosuppressive and anti-tumor properties. Cirsilineol significantly ameliorates trinitro-benzene sulfonic acid (TNBS)-induced T-cell-mediated experimental colitis in mice[1].
Related Catalog
Signaling Pathways >>Stem Cell/Wnt >>STATResearch Areas >>CancerSignaling Pathways >>Immunology/Inflammation >>IFNARSignaling Pathways >>JAK/STAT Signaling >>STATResearch Areas >>Inflammation/Immunology
In Vitro
Cirsilineol (0.1-10 μM; 96 hours) single mixed lymphocyte reaction and Con A-induced T-cell proliferation in a dose-dependent manner. Cirsilineol (10 μM) does not affect T lymphocyte's viability. The inhibition of cirsilineol (10 μM) on T-cell proliferation is not due to a cytotoxic activity[1]. Cirsilineol (1-10 μM; pretreatment for 3 hours) completely inhibits IFN-γ-induced Tyr701 phosphorylation of STAT1 and JAK2 activation[1]. Cell Proliferation Assay[1] Cell Line: Splenocytes Concentration: 0.1, 1, 10 μM Incubation Time: 96 hours Result: Inhibited single mixed lymphocyte reaction and Concanavalin A (Con A; 5 μg/mL; for 72 h)-induced T-cell proliferation in a dose-dependent manner. Suppressed OVA323-339-specific CD4+ T-cell proliferation. Western Blot Analysis[1] Cell Line: Splenic CD4+ T cells Concentration: 1, 5, 10 μM Incubation Time: Pretreatment for 3 hours Result: Completely inhibited IFN-γ-induced (25 ng; 30 mins) Tyr701 phosphorylation of STAT1 and JAK2 activation. Suppressed IFN-γ-induced (25 ng; 12 hours) Th1-specific transcription factor T-bet.
In Vivo
cirsilineol (3, 10, and 30 mg/kg) significantly ameliorates TNBS-induced Th1-mediated colitis through inhibiting IFN-γ/STAT1/T-bet signaling in CD4+ T cells. Animal Model: 8-10-week-old female C57BL/6, BALB/c and DO11.10 transgenic mice with TNBS (10 mg; 100 μL)[1] Dosage: 3, 10, 30 mg/kg Administration: IP; daily; for 11 days Result: Showed a significant improved effect on the body weights and survival rate of mice. Markedly reduced inflammatory infiltration, restoration of the destructive mucosal architecture and remission of edema.
References
[1]. Yang Sun, et al. Novel immunomodulatory properties of cirsilineol through selective inhibition of IFN-gamma signaling in a murine model of inflammatory bowel disease. Biochem Pharmacol. 2010 Jan 15;79(2):229-38.
Chemical & Physical Properties
Density
1.387g/cm3
Boiling Point
585ºC at 760 mmHg
Molecular Formula
C18H16O7
Molecular Weight
344.31500
Flash Point
215.2ºC
Exact Mass
344.09000
PSA
98.36000
LogP
2.89700
Index of Refraction
1.627
InChIKey
VKOSQMWSWLZQPA-UHFFFAOYSA-N
SMILES
COc1cc(-c2cc(=O)c3c(O)c(OC)c(OC)cc3o2)ccc1O
Storage condition
?20°C
Safety Information
RIDADR
NONH for all modes of transport
HS Code
2914509090
Customs
HS Code
2914509090
Summary
HS:2914509090 other ketones with other oxygen function VAT:17.0% Tax rebate rate:9.0% Supervision conditions:none MFN tariff:5.5% General tariff:30.0%
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