CAS 298-93-1|Thiazolyl Blue

Introduction：Basic information about CAS 298-93-1|Thiazolyl Blue, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Table of Contents

1. Names
2. Thiazolyl Blue BiologicalActivity
3. Chemical & Physical Properties
4. Toxicological Information
CHEMICAL IDENTIFICATION
HEALTH HAZARD DATA
ACUTE TOXICITY DATA
MUTATION DATA
5. Safety Information
6. Customs
7. Articles3106
8. Synonyms

Common Name	Thiazolyl Blue
CAS Number	298-93-1	Molecular Weight	414.322
Density	/	Boiling Point	/
Molecular Formula	C₁₈H₁₆BrN₅S	Melting Point	195 °C (dec.)(lit.)
MSDS	ChineseUSA	Flash Point	/
Symbol	GHS07, GHS08	Signal Word	Warning

Names

Name	3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide
Synonym	More Synonyms

Thiazolyl Blue BiologicalActivity

Description	MTT is a colorimetric agent widely used to measure cell proliferation. MTT is reduced from yellow color to purple formazan in living cells.
Related Catalog	Signaling Pathways >>Others >>OthersBiochemical Assay ReagentsResearch Areas >>Others
In Vitro	MTT is used in conjunction with rhodamine B to measure mitochondrial membrane potential. MTT-formazan is generated in mitochondria acts as a fluorescence quencher for rhodamine that distributes across membranes of viable cells according to membrane potential. In the absence of mPMS, cellular reduction of MTT is strong. MTT is membrane-impermeable when incorporated into large unilamellar liposomes, and therefore it is taken up by cells via endocytosis[1].
References	[1]. Berridge MV, et al. Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction. Biotechnol Annu Rev. 2005;11:127-52.

Chemical & Physical Properties

Melting Point	195 °C (dec.)(lit.)
Molecular Formula	C₁₈H₁₆BrN₅S
Molecular Weight	414.322
Exact Mass	413.030975
PSA	75.72000
LogP	0.28830
InChIKey	AZKSAVLVSZKNRD-UHFFFAOYSA-M
SMILES	Cc1nc(-[n+]2nc(-c3ccccc3)nn2-c2ccccc2)sc1C.[Br-]
Storage condition	2-8°C
Water Solubility	Slightly soluble

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: XF8060000

CHEMICAL NAME :: 2H-Tetrazolium, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-, bromide

CAS REGISTRY NUMBER :: 298-93-1

LAST UPDATED :: 199701

DATA ITEMS CITED :: 4

MOLECULAR FORMULA :: C18-H16-N5-S.Br

MOLECULAR WEIGHT :: 414.36

WISWESSER LINE NOTATION :: T5NNKNJ BR& ER& C- BT5N CSJ D1 E1 &E

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

TYPE OF TEST :: Mutation in microorganisms

TEST SYSTEM :: Bacteria - Escherichia coli

DOSE/DURATION :: 5 ug/plate

REFERENCE :: MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 68,107,1979 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X1328 No. of Facilities: 93 (estimated) No. of Industries: 1 No. of Occupations: 3 No. of Employees: 1106 (estimated) No. of Female Employees: 985 (estimated)

Safety Information

Symbol	GHS07, GHS08
Signal Word	Warning
Hazard Statements	H315-H319-H335-H341
Precautionary Statements	P261-P281-P305 + P351 + P338
Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	Xi: Irritant;
Risk Phrases	R46
Safety Phrases	S22-S24/25
RIDADR	NONH for all modes of transport
WGK Germany	3
RTECS	XF8060000
HS Code	29341000

Customs

HS Code	29341000

Articles3106

Neuroprotective effect of modified Chungsimyeolda-tang, a traditional Korean herbal formula, via autophagy induction in models of Parkinson's disease.

J. Ethnopharmacol. 159 , 93-101, (2014)

Previous studies in our laboratory revealed the neuroprotective effect of modified Yeoldahanso-tang (MYH) in models of Parkinson׳s disease (PD). In this study, we investigated another traditional Kore...

Pro-apoptotic and pro-autophagic effects of the Aurora kinase A inhibitor alisertib (MLN8237) on human osteosarcoma U-2 OS and MG-63 cells through the activation of mitochondria-mediated pathway and inhibition of p38 MAPK/PI3K/Akt/mTOR signaling pathway.

Drug Des. Devel. Ther. 9 , 1555-84, (2015)

Osteosarcoma (OS) is the most common malignant bone tumor occurring mostly in children and adolescents between 10 and 20 years of age with poor response to current therapeutics. Alisertib (ALS, MLN823...

Alisertib, an Aurora kinase A inhibitor, induces apoptosis and autophagy but inhibits epithelial to mesenchymal transition in human epithelial ovarian cancer cells.

Drug Des. Devel. Ther. 9 , 425-64, (2015)

Ovarian cancer is a leading killer of women, and no cure for advanced ovarian cancer is available. Alisertib (ALS), a selective Aurora kinase A (AURKA) inhibitor, has shown potent anticancer effects, ...

Synonyms

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium Bromide

ThiazolylBlue(Mtt)

MttA.R.

2H-Tetrazolium, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-, bromide (1:1)

3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium Bromide

3-(4,5-Dimethyl-1,3-thiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium bromide

Methylthiazoletetrazolium bromide

Thiazolyl Blue

2H-tetrazolium, 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-, bromide (1:1)

2-(4,5-Dimethyl-1,3-thiazol-2-yl)-3,5-diphenyl-2H-tetrazol-3-ium bromide

MFCD00011964

EINECS 206-069-5

THIAZOLE BLUE

Thiazolyl blue tetrazolium bromide (MTT)

thiazolyl blue tetrazolium bromide

mmttetrazolium

3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyl-2H-Tetrazolium Bromide

MTT

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