CAS 85138-49-4|BCECF
| Common Name | BCECF | ||
|---|---|---|---|
| CAS Number | 85138-49-4 | Molecular Weight | 520.44100 |
| Density | 1.67g/cm3 | Boiling Point | 936.2ºC at 760mmHg |
| Molecular Formula | C27H20O11 | Melting Point | / |
| MSDS | USA | Flash Point | 318.2ºC |
Names
| Name | 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein |
|---|---|
| Synonym | More Synonyms |
BCECF BiologicalActivity
| Description | BCECF is a duorescent dye with λexcitation of 420~520 nm and λemission of 530 nm. |
|---|---|
| Related Catalog | Signaling Pathways >>Others >>OthersDye ReagentsResearch Areas >>Others |
| In Vitro | Fully treated cells show hydrogenosomes with an electron-dense deposit which aggregates to a variable extent.The staining is seen in the interior of hydrogenosomes in some instances. It is also observed by microscopy that the K+/H+ ionophor nigericin does not inhibit hydrogenosomal loading with BCECF[1]. |
| Kinase Assay | The cells are palced in a petri dish and BCECF present in trichomonads is fixed for electron microsopy. Controns without BCECF loading, without DAB treatment, and without UV illumination are run in parallel. Trichomonads remaine viable during the procedure, except that without BCECF they are dead and disrupted by 4 h. Then the cell are lysed. After lysis, BCECF is added to the lysate to 20 μM, and the lysate is incubated,followed by 3 washes in lysis buffer to remove free dye and resuspension of the pellet in the original volume[1]. |
| References | [1]. Scott DA, et al. Analysis of the uptake of the fluorescent marker 2',7'-bis-(2-carboxyethyl)-5(and-6)-carboxyfluorescein (BCECF) by hydrogenosomes in Trichomonas vaginalis. Eur J Cell Biol. 1998 Jun;76(2):139-45. |
Chemical & Physical Properties
| Density | 1.67g/cm3 |
|---|---|
| Boiling Point | 936.2ºC at 760mmHg |
| Molecular Formula | C27H20O11 |
| Molecular Weight | 520.44100 |
| Flash Point | 318.2ºC |
| Exact Mass | 520.10100 |
| PSA | 187.89000 |
| LogP | 3.39840 |
| Index of Refraction | 1.743 |
| Storage condition | -20°C |
Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| Hazard Codes | Xn |
| Risk Phrases | 20/21/22-36/37/38 |
| Safety Phrases | 22-24/25 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3.0 |
Articles52
More Articles| Local pH domains regulate NHE3-mediated Na⁺ reabsorption in the renal proximal tubule. Am. J. Physiol. Renal Physiol. 307(11) , F1249-62, (2014) The proximal tubule Na(+)/H(+) exchanger 3 (NHE3), located in the apical dense microvilli (brush border), plays a major role in the reabsorption of NaCl and water in the renal proximal tubule. In resp... | |
| Role of polyol moiety of amphotericin B in ion channel formation and sterol selectivity in bilayer membrane. Bioorg. Med. Chem. 23 , 5782-8, (2015) Amphotericin B (AmB) is a polyene macrolide antibiotic widely used to treat mycotic infections. In this paper, we focus on the role of the polyol moiety of AmB in sterol selectivity using 7-oxo-AmB, 7... | |
| A novel effect of DMOG on cell metabolism: direct inhibition of mitochondrial function precedes HIF target gene expression. Biochim. Biophys. Acta 1847 , 1254-66, (2015) Abnormal accumulation of oncometabolite fumarate and succinate is associated with inhibition of mitochondrial function and carcinogenesis. By competing with α-ketoglutarate, oncometabolites also activ... |
Synonyms
| 2-[2,7-bis(2-carboxyethyl)-3-hydroxy-6-oxoxanthen-9-yl]terephthalic acid |
| BCECF |
