Introduction：Basic information about CAS 89889-52-1|Biotin-LC-LC-NHS, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
Biotin-LC-LC-NHS is a SMCC cross-linking reagent that can be used to mark antibody and other small molecules, such as Paclitaxel[1][2].
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In Vitro
Biotinylation of antibodies is accomplished by the reaction of Biotin-LC-LC-NHS (1 mg/mL in dimethyl formamide) with the protein in aqueous phase pH 7.4. The molar ratio of biotin to antibody in the labeling reaction was 10:1 (the final concentration of biotin was 70 μM) [1].
References
[1]. K Dill, et al. Immunoassays Based on Electrochemical Detection Using Microelectrode Arrays. Biosens Bioelectron. 2004 Nov 1;20(4):736-42.
[2]. Lev G Lis, et al. Synthesis and Biological Evaluation of a Biotinylated Paclitaxel With an Extra-Long Chain Spacer Arm. ACS Med Chem Lett . 2012 Sep 13;3(9):745-748.
Ligand-based histochemical localization and capture of cells expressing heat-stable enterotoxin receptors.
Mol. Microbiol. 8 , 865-873, (1993)
The heat stable enterotoxins (ST) of enterotoxigenic Escherichia coli (ETEC) cause diarrhoea by binding specific intestinal receptors. Precise histochemical localization of ST receptors could provide ...
