CAS 108964-32-5|Fura-2, AM
| Common Name | Fura-2, AM | ||
|---|---|---|---|
| CAS Number | 108964-32-5 | Molecular Weight | 1001.850 |
| Density | 1.4±0.1 g/cm3 | Boiling Point | 975.9±75.0 °C at 760 mmHg |
| Molecular Formula | C44H47N3O24 | Melting Point | / |
| MSDS | ChineseUSA | Flash Point | 544.0±37.1 °C |
Names
| Name | acetyloxymethyl 2-[5-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-4-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-1-benzofuran-2-yl]-1,3-oxazole-5-carboxylate |
|---|---|
| Synonym | More Synonyms |
Fura-2, AM BiologicalActivity
| Description | Fura-2 AM is a ratiometric fluorescent Ca2+ indicator. |
|---|---|
| Related Catalog | Signaling Pathways >>Others >>OthersDye ReagentsResearch Areas >>Others |
| In Vitro | The trypsin (100 nM) mediated Ca2+ transients are imagined in tsA-201 cells loaded with either 750 nM (n=111), 500 nM (n=119) or 250 nM (n=130) Fura-2 AM and compare the average Ca2+ increase above the baseline to the value obtained in the initial experiments using 1 μM of Fura-2 AM. The Ca2+ increases above the baseline for the 750, 500 and 250 nM are 633.0±33.9 nM (n=111, P>0.05 compare to 1 μM Fura-2 AM), 552.6±22.7 nM (n=119, P>0.05 compare to 1 μM Fura-2 AM) and 616.0±24.9 nM (n=130, P>0.05 compare to 1 μM Fura-2 AM), respectively[1]. |
| Cell Assay | Cells are loaded with Fura-2 AM, which diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid. Aliquot 2-mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1 µM Fura-2 AM solution. Vortex the solution vigorously for 1 minute. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Prepare a 35 mm dish containing 2-mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish. Mount the coverslip on the imaging chamber[2]. |
| References | [1]. Tinning PW, et al. A 340/380 nm light-emitting diode illuminator for Fura-2 AM ratiometric Ca2+ imaging of live cells with better than 5 nM precision. J Microsc. 2017 Aug 24. [2]. Odmara L Barreto-Chang, et al. Calcium Imaging of Cortical Neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23). pii: 1067. |
Chemical & Physical Properties
| Density | 1.4±0.1 g/cm3 |
|---|---|
| Boiling Point | 975.9±75.0 °C at 760 mmHg |
| Molecular Formula | C44H47N3O24 |
| Molecular Weight | 1001.850 |
| Flash Point | 544.0±37.1 °C |
| Exact Mass | 1001.254944 |
| PSA | 327.11000 |
| LogP | 2.90 |
| Vapour Pressure | 0.0±0.3 mmHg at 25°C |
| Index of Refraction | 1.567 |
| InChIKey | VPSRLGDRGCKUTK-UHFFFAOYSA-N |
| SMILES | CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc1cc2cc(-c3ncc(C(=O)OCOC(C)=O)o3)oc2cc1N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O |
| Storage condition | −20°C |
| Water Solubility | DMSO: soluble |
Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
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Synonyms
| Fura 2am |
| Fura-2 pentakis(acetoxymethyl) ester |
| MFCD00036976 |
| Acetoxymethyl 2-(6-{bis[2-(acetoxymethoxy)-2-oxoethyl]amino}-5-[2-(2-{bis[2-(acetoxymethoxy)-2-oxoethyl]amino}-5-methylphenoxy)ethoxy]-1-benzofuran-2-yl)-1,3-oxazole-5-carboxylate |
| Fura-2 AM |
