CAS 81-93-6|Phenosafranine

Introduction：Basic information about CAS 81-93-6|Phenosafranine, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Table of Contents

1. Names
2. Phenosafranine BiologicalActivity
3. Chemical & Physical Properties
4. Toxicological Information
CHEMICAL IDENTIFICATION
HEALTH HAZARD DATA
ACUTE TOXICITY DATA
MUTATION DATA
5. Safety Information
6. Articles27
7. Synonyms

Common Name	Phenosafranine
CAS Number	81-93-6	Molecular Weight	322.792
Density	1.1738 (rough estimate)	Boiling Point	478.49°C (rough estimate)
Molecular Formula	C₁₈H₁₅ClN₄	Melting Point	300ºC
MSDS	ChineseUSA	Flash Point	/
Symbol	GHS07	Signal Word	Warning

Names

Name	safranin O
Synonym	More Synonyms

Phenosafranine BiologicalActivity

Description	Phenosafranine is a phenazine dye. Phenosafranine has high binding affinity to triplex RNA compared to the parent duplex form, binds through intercalation to both forms of RNA. Phenosafranine can be used for staining plant cells, determination of hemoglobin, dopamine, serotonin and so on[1][2][3].
Related Catalog	Research Areas >>OthersSignaling Pathways >>Others >>Others
References	[1]. Widholm JM. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 1972 Jul;47(4):189-94. [2]. Liu, W., et al. Characterization of Phenosafranine–Hemoglobin Interactions in Aqueous Solution. J Solution Chem 40, 231–246 (2011). [3]. Pradhan AB, et al. An overview on the interaction of phenazinium dye phenosafranine to RNA triple and double helices. Int J Biol Macromol. 2016 May;86:345-51.

Description

Phenosafranine is a phenazine dye. Phenosafranine has high binding affinity to triplex RNA compared to the parent duplex form, binds through intercalation to both forms of RNA. Phenosafranine can be used for staining plant cells, determination of hemoglobin, dopamine, serotonin and so on[1][2][3].

Related Catalog

Research Areas >>OthersSignaling Pathways >>Others >>Others

References

[1]. Widholm JM. The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol. 1972 Jul;47(4):189-94.

[2]. Liu, W., et al. Characterization of Phenosafranine–Hemoglobin Interactions in Aqueous Solution. J Solution Chem 40, 231–246 (2011).

[3]. Pradhan AB, et al. An overview on the interaction of phenazinium dye phenosafranine to RNA triple and double helices. Int J Biol Macromol. 2016 May;86:345-51.

Chemical & Physical Properties

Density	1.1738 (rough estimate)
Boiling Point	478.49°C (rough estimate)
Melting Point	300ºC
Molecular Formula	C₁₈H₁₅ClN₄
Molecular Weight	322.792
Exact Mass	322.098511
PSA	68.81000
LogP	0.99550
Index of Refraction	1.6110 (estimate)
InChIKey	SOUHUMACVWVDME-UHFFFAOYSA-N
SMILES	Nc1ccc2nc3ccc(N)cc3[n+](-c3ccccc3)c2c1.[Cl-]

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SG1630000

CHEMICAL NAME :: Phenazinium, 3,7-diamino-5-phenyl-, chloride

CAS REGISTRY NUMBER :: 81-93-6

LAST UPDATED :: 199612

DATA ITEMS CITED :: 5

MOLECULAR FORMULA :: C18-H15-N4.Cl

MOLECULAR WEIGHT :: 322.82

WISWESSER LINE NOTATION :: T C666 BK INJ BR& EZ MZ &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 112 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 18 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

MUTATION DATA

TEST SYSTEM :: Insect - Drosophila melanogaster

DOSE/DURATION :: 1000 ppm

REFERENCE :: AMNTA4 American Naturalist. (Univ. of Chicago Press, Journals Div., POB 37005, Chicago, IL 60637) V.1- 1867- Volume(issue)/page/year: 87,295,1953

Safety Information

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H315-H319-H335
Precautionary Statements	P261-P305 + P351 + P338
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xi: Irritant;
Risk Phrases	R36/37/38
Safety Phrases	26-36
RIDADR	NONH for all modes of transport
WGK Germany	3
RTECS	SG1630000

Articles27

Spectrophotometric and spectrofluorometric studies on interaction of cationic dyes with bacterial capsular polysaccharide.

Indian J. Biochem. Biophys. 35(4) , 241-6, (1998)

Interaction of Klebsiella K14 capsular polysaccharide with cationic dyes pinacyanol chloride, acridine orange and phenosafranin has been studied by spectrophotometric and spectrofluorometric technique...

Sulfite Oxidase Catalyzes Single-Electron Transfer at Molybdenum Domain to Reduce Nitrite to Nitric Oxide.

Antioxid. Redox Signal. 23 , 283-94, (2015)

Recent studies suggest that the molybdenum enzymes xanthine oxidase, aldehyde oxidase, and mARC exhibit nitrite reductase activity at low oxygen pressures. However, inhibition studies of xanthine oxid...

A promising dehydrogenase-based bioanode for a glucose biosensor and glucose/O2 biofuel cell.

Analyst 137(9) , 2233-8, (2012)

A new type of dehydrogenase-based amperometric glucose biosensor was constructed using glucose dehydrogenase (GDH) which was immobilized on the edge-plane pyrolytic graphite (EPPG) electrode modified ...

Synonyms

Phenazinium, 3,7-diamino-5-phenyl-, chloride (1:1)

Phenosafranin

3,7-diamino-5-phenyl phenazinium chloride

MFCD00036335

Phenosafranine

3,7-Diamino-5-phenylphenazin-5-ium chloride

EINECS 201-387-0

Phenosofranine

Safrinin B Extra

Phenosafrinine

Phenosofrinine

Schultz No. 958

Safranin B Extra

10-phenylphenazin-10-ium-2,8-diamine,chloride

Phenosafranine,chloride

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