CAS 2869-83-2|Janus Green B

Introduction：Basic information about CAS 2869-83-2|Janus Green B, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Table of Contents

1. Names
2. Janus Green B BiologicalActivity
3. Chemical & Physical Properties
4. Toxicological Information
CHEMICAL IDENTIFICATION
HEALTH HAZARD DATA
ACUTE TOXICITY DATA
MUTATION DATA
5. Safety Information
6. Customs
7. Articles20
8. Synonyms

Common Name	Janus Green B
CAS Number	2869-83-2	Molecular Weight	511.060
Density	/	Boiling Point	/
Molecular Formula	C₃₀H₃₁ClN₆	Melting Point	160 °C (dec.)(lit.)
MSDS	USA	Flash Point	/

Names

Name	Janus Green B chloride
Synonym	More Synonyms

Janus Green B BiologicalActivity

Description	Janus green B is a supravital stain. Janus green B staining reaction is oxygen dependent, and is reversibly inhibited by cyanide. Janus green B has been used for staining peripheral nerves in live insects, lymphatic vessels of rabbits and mitochondria[1][2][3].
Related Catalog	Research Areas >>OthersSignaling Pathways >>Others >>Others
References	[1]. LAZAROW A, et al. Studies on the enzymatic basis for the Janus green B staining reaction. J Histochem Cytochem. 1953 Jul;1(4):234-41. [2]. Yack JE. Janus Green B as a rapid, vital stain for peripheral nerves and chordotonal organs in insects. J Neurosci Methods. 1993 Aug;49(1-2):17-22. [3]. Lee BC, et al. Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method. Anat Rec B New Anat. 2005 Sep;286(1):1-7.

Description

Janus green B is a supravital stain. Janus green B staining reaction is oxygen dependent, and is reversibly inhibited by cyanide. Janus green B has been used for staining peripheral nerves in live insects, lymphatic vessels of rabbits and mitochondria[1][2][3].

Related Catalog

Research Areas >>OthersSignaling Pathways >>Others >>Others

References

[1]. LAZAROW A, et al. Studies on the enzymatic basis for the Janus green B staining reaction. J Histochem Cytochem. 1953 Jul;1(4):234-41.

[2]. Yack JE. Janus Green B as a rapid, vital stain for peripheral nerves and chordotonal organs in insects. J Neurosci Methods. 1993 Aug;49(1-2):17-22.

[3]. Lee BC, et al. Novel threadlike structures (Bonghan ducts) inside lymphatic vessels of rabbits visualized with a Janus Green B staining method. Anat Rec B New Anat. 2005 Sep;286(1):1-7.

Chemical & Physical Properties

Melting Point	160 °C (dec.)(lit.)
Molecular Formula	C₃₀H₃₁ClN₆
Molecular Weight	511.060
Exact Mass	510.229858
PSA	47.97000
LogP	3.99630
Vapour Pressure	2.6 mm Hg ( 20 °C)
InChIKey	XXACTDWGHQXLGW-UHFFFAOYSA-M
SMILES	CCN(CC)c1ccc2nc3ccc(N=Nc4ccc(N(C)C)cc4)cc3[n+](-c3ccccc3)c2c1.[Cl-]
Water Solubility	H2O: 1 M hot, clear, colorless

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: SG1633000

CHEMICAL NAME :: Phenazinium, 3-(diethylamino)-7-((p-(dimethylamino)phenyl)azo)-5-p henyl-, chloride

CAS REGISTRY NUMBER :: 2869-83-2

LAST UPDATED :: 199701

DATA ITEMS CITED :: 5

MOLECULAR FORMULA :: C30-H31-N6.Cl

MOLECULAR WEIGHT :: 511.12

WISWESSER LINE NOTATION :: T C666 BK INJ BR& ENUNR DN1&1& MN2&2 &G

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Subcutaneous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 960 mg/kg/13W-I

TOXIC EFFECTS :: Tumorigenic - equivocal tumorigenic agent by RTECS criteria Tumorigenic - tumors at site of application

MUTATION DATA

TYPE OF TEST :: Cytogenetic analysis

TEST SYSTEM :: Rodent - hamster Ovary

DOSE/DURATION :: 20 umol/L/5H (Continuous)

REFERENCE :: ENMUDM Environmental Mutagenesis. (New York, NY) V.1-9, 1979-87. For publisher information, see EMMUEG. Volume(issue)/page/year: 1,27,1979 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - X1535 No. of Facilities: 181 (estimated) No. of Industries: 5 No. of Occupations: 5 No. of Employees: 2102 (estimated) No. of Female Employees: 188 (estimated)

Safety Information

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard Codes	T: Toxic;
Risk Phrases	R60
Safety Phrases	S22-S24/25
RIDADR	NONH for all modes of transport
WGK Germany	3
RTECS	SG1633000
HS Code	32041900

Customs

HS Code	32041900

Articles20

Induction of apoptotic cell death by betulin in multidrug-resistant human renal carcinoma cells.

Oncol. Rep. 34 , 1058-64, (2015)

Betulin, a triterpene from the bark of various species of birch tree, has various biological effects, including antiviral, antifungal and anticancer activities. The aim of the present study was to elu...

Analysis and testing of biological stains--the Biological Stain Commission Procedures.

Biotech. Histochem. 77(5&6) , 237-275, (2002)

Complete staining of human spermatozoa and immature germ cells combined with phase contrast microscopy.

Arch. Androl. 19(3) , 217-21, (1987)

A method combining Janus green B and Thymol blue stains the anterior part of the head, the nuclear membrane, middle piece, and tail of spermatozoa light green and the nucleus deep purple. The method p...

Synonyms

3-(Diethylamino)-7-{(E)-[4-(dimethylamino)phenyl]diazenyl}-5-phenylphenazin-5-ium chloride

Phenazinium, 3-(diethylamino)-7-[(E)-2-[4-(dimethylamino)phenyl]diazenyl]-5-phenyl-, chloride (1:1)

Janus Green V

Janus Green B,high purity biological stain

8-[[4-(dimethylamino)phenyl]diazenyl]-N,N-diethyl-10-phenylphenazin-10-ium-2-amine,chloride

Janus Green B

EINECS 220-695-6

MFCD00011337

3-(Diethylamino)-7-[[4-(dimethylamino)phenyl]azo]-5-phenylphenazinium Chloride

diazin green s

Phenazinium, 3- (diethylamino)-7-[[p-(dimethylamino)phenyl]azo]-5-phenyl-, chloride

Phenazinium, 3- (diethylamino)-7-[[4-(dimethylamino)phenyl]azo]-5-phenyl-, chloride

Union Green B

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