CAS 110448-33-4|ML-7 hydrochloride
| Common Name | ML-7 hydrochloride | ||
|---|---|---|---|
| CAS Number | 110448-33-4 | Molecular Weight | 452.738 |
| Density | / | Boiling Point | 542.7ºC at 760 mmHg |
| Molecular Formula | C15H18ClIN2O2S | Melting Point | 246-249ºC dec. |
| MSDS | USA | Flash Point | 282ºC |
Names
| Name | 1-((5-Iodonaphthalen-1-yl)sulfonyl)-1,4-diazepane hydrochloride |
|---|---|
| Synonym | More Synonyms |
ML-7 hydrochloride BiologicalActivity
| Description | ML-7 hydrochloride is a naphthalene sulphonamide derivative, potently inhibits MLCK (IC50=300 nM) and TRPC6 channel (IC50>10 μM). |
|---|---|
| Related Catalog | Signaling Pathways >>Cytoskeleton >>MyosinResearch Areas >>Cancer |
| Target | IC50: 300 nM (MLCK)[1] |
| In Vitro | ML-7 hydrochloride inhibits rabbit portal vein α1-adrenoceptor NSCC with IC50 of 0.8 μM[1]. The myosin light chain kinase (MLCK) inhibitor ML-7 hydrochloride (3 μ M and 10 μM) also attenuates the Dexmedetomidine (DMT)-induced contraction (p<0.05 versus control)[2]. |
| In Vivo | In sham operated animals Evans Blue extravasation is not different between ML-7 hydrochloride and vehicle group (sham+vehicle: 0.26±0.02 OD/g; sham+ML-7: 0.26±0.02 OD/g). After CCI inhibition of MLCK with ML-7 results in a significant lower amount of intracerebral Evans Blue compared to vehicle treated animals (CCI+vehicle: 0.42±0.04 OD/g; CCI+ML-7: 0.35±0.05 OD/g, p=0.048)[3]. |
| Animal Admin | Mice[3] Mice are treated with intraperitoneal injection of the selective MLCK inhibitor ML-7 (1 mg/kg) or vehicle solution (0.9% NaCl,control group) 1 h prior to and 6 h after trauma. Hemispheric brain water content and neurological function are measured 24 h after controlled cortical impact (CCI) in animals randomized to: (i) vehicle+sham surgery, (ii) vehicle+CCI, (iii) ML-7+shamsurgery or (iv) ML-7+CCI (n=8 per group) and 15 min after CCI in animals randomized to: (i) vehicle+sham surgery, (ii) vehicle+CCI, (iii) ML-7+sham surgery or (iv) ML-7+CCI (n=6 per group). Mice are treated with intraperitoneal injection of the selective MLCK inhibitor ML-7 (1 mg/kg) or vehicle solution (0.9% NaCl, control group) 1 h prior to and 6 h after trauma. Evans Blue extravasation is determined 24 h after surgery in animals randomized to: (i) vehicle + CCI or (ii) ML-7+CCI (n=5 per group) and in animals randomized to (iii) vehicle + sham surgery or (iv) ML-7+sham surgery (n=6 per group). ICP is measured 24 h CCI and sham surgery. |
| References | [1]. Shi J, et al. Myosin light chain kinase-independent inhibition by ML-9 of murine TRPC6 channels expressed in HEK293cells. Br J Pharmacol. 2007 Sep;152(1):122-31. [2]. Yu J, et al. Dexmedetomidine-Induced Contraction in the Isolated Endothelium-Denuded Rat Aorta Involves PKC-δ-mediated JNK Phosphorylation. Int J Med Sci. 2015 Sep 4;12(9):727-36. [3]. Luh C, et al. Inhibition of myosin light chain kinase reduces brain edema formation after traumatic brain injury. J Neurochem. 2010 Feb;112(4):1015-25. |
Chemical & Physical Properties
| Boiling Point | 542.7ºC at 760 mmHg |
|---|---|
| Melting Point | 246-249ºC dec. |
| Molecular Formula | C15H18ClIN2O2S |
| Molecular Weight | 452.738 |
| Flash Point | 282ºC |
| Exact Mass | 451.982208 |
| PSA | 57.79000 |
| LogP | 4.57790 |
| InChIKey | KDDALCDYHZIZMH-UHFFFAOYSA-N |
| SMILES | Cl.O=S(=O)(c1cccc2c(I)cccc12)N1CCCNCC1 |
| Storage condition | -20℃ |
Safety Information
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
|---|---|
| RIDADR | NONH for all modes of transport |
Articles9
More Articles| Myosin II controls cellular branching morphogenesis and migration in three dimensions by minimizing cell-surface curvature. Nat. Cell Biol. 17(2) , 137-47, (2015) In many cases, cell function is intimately linked to cell shape control. We used endothelial cell branching morphogenesis as a model to understand the role of myosin II in shape control of invasive ce... | |
| Directional bleb formation in spherical cells under temperature gradient. Biophys. J. 109 , 355-64, (2015) Living cells sense absolute temperature and temporal changes in temperature using biological thermosensors such as ion channels. Here, we reveal, to our knowledge, a novel mechanism of sensing spatial... | |
| MAPK3/1 (ERK1/2) and Myosin Light Chain Kinase in Mammalian Eggs Affect Myosin-II Function and Regulate the Metaphase II State in a Calcium- and Zinc-Dependent Manner. Biol. Reprod. 92 , 146, (2015) Vertebrate eggs are arrested at metaphase of meiosis II, a state classically known as cytostatic factor arrest. Maintenance of this arrest until the time of fertilization and then fertilization-induce... |
Synonyms
| 1-(5-Iodonaphthalene-1-sulfonyl)-1H-hexahydro-1,4-diazepine hydrochloride |
| ML-7 HYDROCHLORIDE |
| 1H-1,4-Diazepine, hexahydro-1-[(5-iodo-1-naphthalenyl)sulfonyl]-, hydrochloride (1:1) |
| MFCD00065524 |
| 1-[(5-Iodo-1-naphthyl)sulfonyl]-1,4-diazepane hydrochloride (1:1) |
| ML-7 (hydrochloride) |
