Introduction：Basic information about CAS 10205-62-6|BTA-2, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | BTA-2 |
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| CAS Number | 10205-62-6 | Molecular Weight | 268.37700 |
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| Density | 1.188g/cm3 | Boiling Point | 428.8ºC at 760mmHg |
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| Molecular Formula | C16H16N2S | Melting Point | 204-206ºC(lit.) |
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| MSDS | / | Flash Point | 213.1ºC |
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Names
| Name | N,N-dimethyl-4-(6-methyl-1,3-benzothiazol-2-yl)aniline |
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| Synonym | More Synonyms |
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BTA-2 BiologicalActivity
| Description | BTA-2, a benzothiazole dye, is structurally similar to thioflavin T (ThT), which exhibits an enhanced fluorescence signal when bound to amyloid fibrils. BTA-2 has distinct absorption and emission characteristics in solution and when bound to amyloid fibrils, which makes it can used for identifying amyloid fibrils using spectroscopy[1]. |
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| Related Catalog | Research Areas >>OthersSignaling Pathways >>Others >>Others |
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| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). To research how CH3CN affects binding of amyloid fibrils (varied the amount of CH3CN but maintained the same concentration of BTA-2, fibrils, and water) and how the concentration of BTA-2 affects binding (varied the amount of BTA-2 but had a constant amount of CH3CN, fibrils, and water) [1] . BTA-2 in solution and in the presence of amyloid fibrils: 1. Insulin obtains from a bovine pancreas, and is stored at −20°C in a desiccator. 2. To synthesize BTA-2 and further purified. 3. To prepare fibrils: Insulin is dissolved in pH 2 water (5 mg/mL) and filters through a 0.2 μm filter, heats at 60℃ for 24 h. Centrifuge at 3000 rpm for 2.5 min to remove any globular artifacts. The supernatant containing the fibrils is saved for later use. Solutions are stored in a refrigerator (10℃) until needed. 4. BTA-2/fibrils solutions (are prepared in two ways): (First way) Diluting (100 μL) of the stock fibril solution with 880 μL of pH 2 water, adding (20 μL) of 0.8 mg/mL (3.15 mM) BTA-2 in CH3CN. (Second way) Diluting (100 μL) of the stock fibril solution with a 1:1 mixture of nanopure water: CH3CN, adding (20 μL) of 0.8 mg/mL (3.15 mM) BTA-2. 5. Two similar solutions are made that contained only the BTA-2 without the fibrils. 6. The standard amyloid fluorescent marker is thioflavin T (ThT): 20 μL of 0.8 mg/mL (2.51 mM) ThT in CH3CN and 100 μL of stock fibril solutions is diluted with 880 μL of pH 2 water.7. The emission spectra of BTA-2 in pH 2 water excited at 430 nm and excited at 360 nm, respectively. |
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| References | [1]. Catherine C Kitts, et al. A spectroscopic study of 2-[4'-(dimethylamino)phenyl]-benzothiazole binding to insulin amyloid fibrils. J Fluoresc. 2010 Jul;20(4):881-9. |
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Chemical & Physical Properties
| Density | 1.188g/cm3 |
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| Boiling Point | 428.8ºC at 760mmHg |
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| Melting Point | 204-206ºC(lit.) |
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| Molecular Formula | C16H16N2S |
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| Molecular Weight | 268.37700 |
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| Flash Point | 213.1ºC |
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| Exact Mass | 268.10300 |
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| PSA | 44.37000 |
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| LogP | 4.33770 |
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| Vapour Pressure | 1.48E-07mmHg at 25°C |
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| Index of Refraction | 1.669 |
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| InChIKey | OEOPVJYUCSQVMJ-UHFFFAOYSA-N |
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| SMILES | Cc1ccc2nc(-c3ccc(N(C)C)cc3)sc2c1 |
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Safety Information
| Hazard Codes | Xi: Irritant; |
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| Risk Phrases | 36/37/38 |
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| Safety Phrases | 26-37/39 |
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| HS Code | 2934999090 |
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Customs
| HS Code | 2934999090 |
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| Summary | 2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |
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Synonyms
| 2-<4-Dimethylamino-phenyl>-6-methyl-benzothiazol |
| MFCD00192069 |
| EINECS 233-506-7 |
