CAS 10127-02-3|Basic Orange 14
| Common Name | Basic Orange 14 | ||
|---|---|---|---|
| CAS Number | 10127-02-3 | Molecular Weight | 739.943 |
| Density | / | Boiling Point | 468.6ºC at 760 mmHg |
| Molecular Formula | C34H40Cl4N6Zn | Melting Point | 165 °C |
| MSDS | ChineseUSA | Flash Point | 237.2ºC |
| Symbol | GHS08 | Signal Word | Warning |
Names
| Name | Basic Orange 14 |
|---|---|
| Synonym | More Synonyms |
Basic Orange 14 BiologicalActivity
| Description | Acridine Orange (Euchrysine 3RX) zinc chloride salt is a cell-penetrable nucleic acid-selective fluorescent dye. Acridine Orange zinc chloride salt produces orange fluorescence when it binds to ssDNA or RNA, and green fluorescence when it binds to dsDNA (Ex: 488 nM; Em: green fluorescence at 530 nm, orange fluorescence at 640 nm)[1][2][3]. |
|---|---|
| Related Catalog | Research Areas >>Others |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[4]. 1. 用 Acridine Orange zinc chloride salt 染色细胞 (1 μM; 20 分钟; 37 ℃)。 2. 用 PBS 洗涤细胞。 3. 通过共聚焦激光扫描显微镜 (FV3000，Olympus) 观察细胞。 |
| In Vivo | Acridine Orange (0.1 mg/kg，静脉注射，狗) zinc chloride salt 在 30 天内没有表现出临床毒性迹象，血液中也没有发现异常[1]。 |
| References | [1]. McMaster GK, et al. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarosegels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8. [2]. Traganos F, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. J Histochem Cytochem. 1977 Jan;25(1):46-56. [3]. Byvaltsev VA, et al. Acridine Orange: A Review of Novel Applications for Surgical Cancer Imaging and Therapy. Front Oncol. 2019 Sep 24;9:925. [4]. Wang Q, et al. Substrate stiffness regulates the differentiation profile and functions of osteoclasts via cytoskeletal arrangement. Cell Prolif. 2022 Jan;55(1):e13172. |
Chemical & Physical Properties
| Boiling Point | 468.6ºC at 760 mmHg |
|---|---|
| Melting Point | 165 °C |
| Molecular Formula | C34H40Cl4N6Zn |
| Molecular Weight | 739.943 |
| Flash Point | 237.2ºC |
| Exact Mass | 736.135986 |
| PSA | 38.74000 |
| LogP | 10.02050 |
| InChIKey | VADJQOXWNSPOQA-UHFFFAOYSA-L |
| SMILES | CN(C)c1ccc2cc3ccc(N(C)C)cc3nc2c1.Cl.Cl[Zn]Cl |
| Storage condition | Store at RT. |
| Water Solubility | soluble |
Safety Information
| Symbol | GHS08 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H341 |
| Precautionary Statements | P281 |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
| Hazard Codes | Xn:Harmful; |
| Risk Phrases | R20/21/22;R68 |
| Safety Phrases | S45-S36/37 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| RTECS | AR7600000 |
| Packaging Group | I; II; III |
| Hazard Class | 6.1 |
| HS Code | 32041300 |
Customs
| HS Code | 32041300 |
|---|
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Synonyms
| N,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc (2:1) dihydrochloride |
| Acridine Orange hemi(zinc chloride) salt |
| MFCD00081043 |
| zinc,3-N,3-N,6-N,6-N-tetramethylacridine-3,6-diamine,dichloride,hydrochloride |
| Acridine Orange |
| N,N,N',N'-Tetramethyl-3,6-acridinediamine - dichlorozinc hydrochloride (2:1:2) |
| Euchrysin GNY |
| EINECS 200-614-0 |
