CAS 87687-02-3|Resorufin benzyl ether

Introduction：Basic information about CAS 87687-02-3|Resorufin benzyl ether, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Table of Contents

1. Names
2. Resorufin benzyl ether BiologicalActivity
3. Chemical & Physical Properties
4. Safety Information
5. Articles22
6. Synonyms

Common Name	Resorufin benzyl ether
CAS Number	87687-02-3	Molecular Weight	303.31100
Density	1.26 g/cm3	Boiling Point	479.9ºC at 760 mmHg
Molecular Formula	C₁₉H₁₃NO₃	Melting Point	/
MSDS	ChineseUSA	Flash Point	/
Symbol	GHS07	Signal Word	Warning

Names

Name	Resorufin benzyl ether,Benzyloxyresorufin
Synonym	More Synonyms

Resorufin benzyl ether BiologicalActivity

Description	Resorufin benzyl ether (BzRes), a fluorogenic enzyme substrate, can be used to detect CYP3A4 enzyme activity. Resorufin benzyl ether modified with a recognizing moiety boronate, can be used for ONOO- detection via a self-immolation mechanism. Ex/Em=530-570 nm/590 nm[1][2][3].
Related Catalog	Research Areas >>Others
In Vitro	CYP3A4 活性测定[2]: 1. 准备反应溶液。 a. 标准原液含有 1 mM Resorufin benzyl ether，以其为荧光底物。将 5mg Resorufin benzyl ether 溶解在 1mL 2% w/v 的 Poloxamer 188 (HY-D1005A)、500 mL Dimethyl sulfoxide (HY-Y0320) 和 3.5 mL 乙腈的混合物中。b. 新鲜配制 CYP3A4 酶液。将 5mL 1mM 酶原液与 995 mL 缓冲液稀释，得到 5 nM 酶溶液。2. 测定 CYP3A4 活性 a. 在 96 孔板中反应。在每个孔中加入 99 mL 缓冲混合液，1 mL 1 mM Resorufin benzyl ether。调整至最终浓度 5 mM。 b. 在 37℃ 孵育 30 分钟之前，转移 100 mL 5 nM 酶液。 c. 在激发波长 (lex=570 nm) 和发射波长 (lem=590 nm) 荧光检测下进行酶活性测定。 d. 影响 CYP3A4 活性测定的因素。如缓冲液 (磷酸盐、Tris-HCl 缓冲液)、缓冲液浓度 (50-200 mM)、培养时间 (0-50 min)。 CYP3A4 代谢活性测定[3]： 1. 加入 CYP3A4 酶至最终浓度为 5 pmol/孔。 2. 每次反应使用 50 pM 底物和 200 mM 磷酸钾缓冲液。< br / >3. 在测量代谢物荧光之前，用 BzRes 孵育 45 分钟。激发波长 (Ex) 为 530 nm，发射波长 (Em) 为590 nm。 4. 将全强度提取物的浓度指定为 100% (在最终测定量中稀释为 1:4)。 5. 以 1:3 重复稀释两次 100% 提取物。计算荧光值的均值和标准差。
References	[1]. Ji X, et al. Regulating the activity of boronate moiety to construct fluorescent probes for the detection of ONOO-in vitro and in vivo. Anal Methods. 2022 Dec 15;14(48):5027-5033. [2]. Nuchtavorn N, et al. Paper-based sol-gel thin films immobilized cytochrome P450 for enzyme activity measurement. Anal Chim Acta. 2020 Feb 15;1098:86-93. [3]. Yale SH, et al. Analysis of the inhibitory potential of Ginkgo biloba, Echinacea purpurea, and Serenoa repens on the metabolic activity of cytochrome P450 3A4, 2D6, and 2C9. J Altern Complement Med. 2005 Jun;11(3):433-9.

Chemical & Physical Properties

Density	1.26 g/cm3
Boiling Point	479.9ºC at 760 mmHg
Molecular Formula	C₁₉H₁₃NO₃
Molecular Weight	303.31100
Exact Mass	303.09000
PSA	52.33000
LogP	3.87180
InChIKey	XNZRYTITWLGTJS-UHFFFAOYSA-N
SMILES	O=c1ccc2nc3ccc(OCc4ccccc4)cc3oc-2c1
Storage condition	2-8°C

Safety Information

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H315-H319-H335
Precautionary Statements	P261-P305 + P351 + P338
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xi: Irritant;
Risk Phrases	R36/37/38
Safety Phrases	26-36
RIDADR	NONH for all modes of transport

Articles22

Modulation of cytochrome P450 2A5 activity by lipopolysaccharide: low-dose effects and non-monotonic dose-response relationship.

PLoS ONE 10(1) , e0117842, (2015)

Mouse cytochrome P450 (CYP) 2A5 is induced by inflammatory conditions and infectious diseases that down-regulate the expression and activity of most other CYP isoforms. Enhanced oxidative stress and n...

Inhibition of cytochrome P450 2B4 by environmentally persistent free radical-containing particulate matter.

Biochem. Pharmacol. 95 , 126-32, (2015)

Combustion processes generate particulate matter (PM) that can affect human health. The presence of redox-active metals and aromatic hydrocarbons in the post-combustion regions results in the formatio...

Inhibition against mosquito cytochrome P450 enzymes by rhinacanthin-A, -B, and -C elicits synergism on cypermethrin cytotoxicity in Spodoptera frugiperda cells.

J. Med. Entomol. 49(5) , 993-1000, (2012)

Rhinacanthus nasutus (Acanthaceae) is a shrub reported to contain insecticidal activities. The current study was conducted to determine whether R. nasutus constituents could inhibit benzyloxyresorufin...

Synonyms

BENZYLOXYRESORUFIN

7-BENZYLOXYRESORUFIN

RESORUFIN BENZYL ETHER

7-Benzyloxyphenoxazone

7-BENZYLOXY-3H-PHENOXAZIN-3-ONE

MFCD00171613

7-Benzyloxy-3H-phenoxazin-3-one,7-Benzyloxyresorufin,O7-Benzylresorufin

Resorufin benzyl ether,7-Benzyloxy-3H-phenoxazin-3-one,7-Benzyloxyresorufin,O7-Benzylresorufin

Benzyloxyresorfin

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