CAS 29270-56-2|4-Fluoro-7-nitrobenzofurazan
| Common Name | 4-Fluoro-7-nitrobenzofurazan | ||
|---|---|---|---|
| CAS Number | 29270-56-2 | Molecular Weight | 183.097 |
| Density | 1.7±0.1 g/cm3 | Boiling Point | 308.6±45.0 °C at 760 mmHg |
| Molecular Formula | C6H2FN3O3 | Melting Point | 52-54 °C(lit.) |
| MSDS | ChineseUSA | Flash Point | 140.4±28.7 °C |
| Symbol | GHS07 | Signal Word | Warning |
Names
| Name | 4-fluoro-7-nitro-2,1,3-benzoxadiazole |
|---|---|
| Synonym | More Synonyms |
4-Fluoro-7-nitrobenzofurazan BiologicalActivity
| Description | NBD-F is a fluorescent derivatization reagent which is originally developed for amino acid analysis. |
|---|---|
| Related Catalog | Signaling Pathways >>Others >>OthersResearch Areas >>OthersDye Reagents |
| In Vitro | As the percentage of the organic phase changes, the retention time of NBD-F remains relatively stable, while the retention times of the derivatization products changes. The pH of the mobile phase affects the separation of the NBD-F and the derivatization products[1]. NBD-F is a fluorescent derivatization reagent that is originally developed for amino acid analysis, and recently applied to the analysis of other amino acid derivatives such as N-methyl-D-aspartic acid and glutathione. The use of NBD-F appears to have several advantages in that the derivatization procedure is simple and its derivatives are highly stable[2]. |
| Kinase Assay | An accurately weighed quantity (0.0183 g) of NBD-F is transferred into a 1 mL centrifuge tube, dissolved in acetonitrile and made up to volume with the same solvent to produce stock solutions of 0.1 M. The solution is protected from light and stored at -20°C until analyzed. A 100 μL aliquot of mixed amino acids solution or sample supernatant, 175 μL of borate buffer solution, 200 μL of acetonitrile and 25 μL of NBD-F working solution are mixed in a 1.5 mL centrifuge tube. The well-mixed solution is allowed to react at 60°C in the water bath for 7 min, excluding light. NBD-F reacts with amino group and enables amino acids to be detected with UV detection. After cooling to room temperature, 10 μL of the solution is injected into the equilibrated HPLC system[1]. |
| References | [1]. Wu X, et al. Determination of amino acid neurotransmitters in rat hippocampi by HPLC-UV using NBD-F as a derivative. Biomed Chromatogr. 2014 Apr;28(4):459-62. [2]. Ishikawa T, et al. Development of an LC-MS/MS method for the analysis of free sphingoid bases using 4-fluoro-7-nitrobenzofurazan (NBD-F). Lipids. 2014 Mar;49(3):295-304. |
Chemical & Physical Properties
| Density | 1.7±0.1 g/cm3 |
|---|---|
| Boiling Point | 308.6±45.0 °C at 760 mmHg |
| Melting Point | 52-54 °C(lit.) |
| Molecular Formula | C6H2FN3O3 |
| Molecular Weight | 183.097 |
| Flash Point | 140.4±28.7 °C |
| Exact Mass | 183.008026 |
| PSA | 84.74000 |
| LogP | 1.27 |
| Vapour Pressure | 0.0±0.6 mmHg at 25°C |
| Index of Refraction | 1.638 |
| InChIKey | PGZIDERTDJHJFY-UHFFFAOYSA-N |
| SMILES | O=[N+]([O-])c1ccc(F)c2nonc12 |
| Storage condition | 2-8°C |
Safety Information
| Symbol | GHS07 |
|---|---|
| Signal Word | Warning |
| Hazard Statements | H315-H319 |
| Precautionary Statements | P305 + P351 + P338 |
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
| Hazard Codes | Xi: Irritant; |
| Risk Phrases | R36/38 |
| Safety Phrases | S26-S36-S39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
| HS Code | 2934999090 |
Customs
| HS Code | 2934999090 |
|---|---|
| Summary | 2934999090. other heterocyclic compounds. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0% |
Articles39
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| Cytotoxic bile acids, but not cytoprotective species, inhibit the ordering effect of cholesterol in model membranes at physiologically active concentrations. Biochim. Biophys. Acta 1828(9) , 2152-63, (2013) Submillimolar concentrations of cytotoxic bile acids (BAs) induce cell death via apoptosis. On the other hand, several cytoprotective BAs were shown to prevent apoptosis in the same concentration rang... | |
| Evaluation of a microchip electrophoresis-mass spectrometry platform deploying a pressure-driven make-up flow. J. Chromatogr. A. 1285 , 159-64, (2013) Integration of a pressure-driven make-up flow (MUF) into a microchip electrophoresis (MCE) platform in order to facilitate its coupling with electrospray ionization-mass spectrometric detection (ESI-M... |
Synonyms
| 7-fluoro-4-nitrobenzofurazan |
| 2,1,3-Benzoxadiazole, 4-fluoro-7-nitro- |
| 4-fluoro-7-nitrobenz-2-oxa-1,3-diazole |
| NBD-F |
| BENZOFURAZAN, 4-FLUORO-7-NITRO- |
| 7-Fluoro-4-nitrobenzo-2-oxa-1,3-diazole |
| 4-fluoro-7-nitro-2,1,3-benzooxadiazole |
| 4-Fluoro-7-nitro-2,1,3-benzoxadiazole |
| MFCD00010196 |
| 4-Fluoro-7-nitrobenzofurazan |
| (4-Fluoro-7-nitro-2,1,3-benzoxadiazole) |
| NBD fluoride |
