CAS 72962-43-7|Epibrassinolide

Introduction：Basic information about CAS 72962-43-7|Epibrassinolide, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Table of Contents

1. Names
2. Epibrassinolide BiologicalActivity
3. Chemical & Physical Properties
4. Safety Information
5. Synonyms

Common Name	Epibrassinolide
CAS Number	72962-43-7	Molecular Weight	480.677
Density	1.1±0.1 g/cm3	Boiling Point	633.7±55.0 °C at 760 mmHg
Molecular Formula	C₂₈H₄₈O₆	Melting Point	200-204ºC
MSDS	/	Flash Point	202.3±25.0 °C

Names

Name	brassinolide
Synonym	More Synonyms

Epibrassinolide BiologicalActivity

Description	Brassinolide is a predominant plant growth modulator that regulate plant cell elongation.
Related Catalog	Signaling Pathways >>Others >>OthersResearch Areas >>CancerNatural Products >>Steroids
Target	plant growth modulator[1]
In Vitro	Brassinolide is a plant sterol first isolated from pollen of rape (Brassica napus L.). Brassinolide can induce a time and concentration-dependent cytotoxicity in PC-3 cells. The mode of cell death appears to be predominately apoptosis, as shown by flow-cytometric analysis, fluorescence and transmission electron microscopes. Caspase-3 activity is obviously increased after Brassinolide treatment. Western blot studies indicate that treatment with Brassinolide triggered a time-dependent decrease in the expression of anti-apoptotic protein Bcl-2, which suggests that Brassinolide can induce cytotoxicity in PC-3 cells by triggering apoptosis. Brassinolide might therefore be a promising candidate for the treatment of prostate cancer[1]. Brassinolide is a plant growth modulator, on multidrug resistance (MDR) of human T lymphoblastoid cell line CCRF-VCR 1000 which is obtained by progressively addition of vincristine (VCR) to sensitive CCRF-CEM cells, and to explore preliminarily the mechanism of reversing action. After treatment of Brassinolide under the concentration of 0.001-10 μg/mL, the resistance of CCRF-VCR is reversed partly with the reversing folds respectively as 4.4-11.6. The intracellular accumulation of rhodamine 123 is significantly reduced in the resistant cells. After treatment of Brassinolide, the accumulation increased, the level of fluorescent dye is situated between resistant cells and sensitive cells. No alteration of the catalytic activity of topoisomerase II is found among three groups. The level of protein expression of p53 in resistant cells is higher than that of sensitive cells. After Brassinolide treatment, the expression of p53 in CCRF-VCR cells restored to the level of sensitive cells. Brassinolide can effectively reverse the resistance of CCRF-VCR cells by inhibiting the effusion of drug transported by P-glucoprotein. To down regulate the abnormal expression of p53 maybe one of the mechanisms of reversing MDR for Brassinolide[2].
Cell Assay	MTT method is used to detect the resistant factor of resistant cell line and the reversing fold after addition of Brassinolide. The intracellular accumulation of rhodamine 123, a fluorescent dye transported by P-glycoprotein is detected by flow cytometry, the catalytic activity of topoisomerase II is assessed by Sulliven method to find the effect of Brassinolide on resistance. The protein expression of p53 is measured using Western blotting in the sensitive cells and resistant cells to explore the effect of Brassinolide[2].
References	[1]. Wu YD, et al. Brassinolide, a plant sterol from pollen of Brassica napus L., induces apoptosis in human prostate cancer PC-3 cells. Pharmazie. 2007 May;62(5):392-5. [2]. Xian LJ, et al. Reversing effect of brassinolide on multidrug resistance of-CCRF-VCR1000 cells and a preliminary investigation on its mechanisms. Yao Xue Xue Bao. 2005 Feb;40(2):117-21.

Chemical & Physical Properties

Density	1.1±0.1 g/cm3
Boiling Point	633.7±55.0 °C at 760 mmHg
Melting Point	200-204ºC
Molecular Formula	C₂₈H₄₈O₆
Molecular Weight	480.677
Flash Point	202.3±25.0 °C
Exact Mass	480.345093
PSA	107.22000
LogP	3.12
Vapour Pressure	0.0±4.2 mmHg at 25°C
Index of Refraction	1.536
InChIKey	IXVMHGVQKLDRKH-KNBKMWSGSA-N
SMILES	CC(C)C(C)C(O)C(O)C(C)C1CCC2C3COC(=O)C4CC(O)C(O)CC4(C)C3CCC12C
Storage condition	−20°C

Safety Information

Hazard Codes	T+
WGK Germany	3

Synonyms

2α,3α,22,23-tetrahydroxy-24-methyl-B-homo-7-oxa-5α-cholestan-6-one

cis,cis-1,6-Dioxa-3,8-cyclodecadien

1,6-dioxa-cyclodeca-3,8-diene

(3aS,5S,6R,7aR,7bS,9aS,10R,12aS,12bS)-10-[(2S,3R,4R,5S)-3,4-Dihydroxy-5,6-dimethyl-2-heptanyl]-5,6-dihydroxy-7a,9a-dimethylhexadecahydro-3H-benzo[c]indeno[5,4-e]oxepin-3-one

(3aS,5S,6R,7aR,7bS,9aS,10R,12aS,12bS)-10-[(2S,3R,4R,5S)-3,4-Dihydroxy-5,6-dimethylheptan-2-yl]-5,6-dihydroxy-7a,9a-dimethylhexadecahydro-3H-benzo[c]indeno[5,4-e]oxepin-3-on

(2α,3α,5α,22R,23R,24S)-2,3,22,23-Tetrahydroxy-B-homo-7-oxaergostan-6-one

2a,3a,22,23-Tetrahydroxy-24-methyl-B-homo-7-oxa-5a-cholestan-6-one

(3aS,5S,6R,7aR,7bS,9aS,10R,12aS,12bS)-10-[(1S,2R,3R,4S)-2,3-dihydroxy-1,4,5-trimethylhexyl]hexadecahydro-5,6-dihydroxy-7a,9a-dimethyl-3H-benzo[c]indeno[5,4-e]oxepin-3-one

3H-Benz[c]indeno[5,4-e]oxepin-3-one, 10-[(1S,2R,3R,4S)-2,3-dihydroxy-1,4,5-trimethylhexyl]hexadecahydro-5,6-dihydroxy-7a,9a-dimethyl-, (3aS,5S,6R,7aR,7bS,9aS,10R,12aS,12bS)-

1,6-Dioxacyclodeca-3,8-dien

3H-Benz[c]indeno[5,4-e]oxepin-3-one, 10-[(1S,2R,3R,4R)-2,3-dihydroxy-1,4,5-trimethylhexyl]hexadecahydro-5,6-dihydroxy-7a,9a-dimethyl-, (3aS,5S,6R,7aR,7bS,9aS,10R,12aS,12bS)-

(1R,3aS,3bS,6aS,8S,9R,10aR,10bS,12aS)-1-[(1S,2R,3R,4S)-2,3-dihydroxy-1,4,5-trimethylhexyl]hexadecahydro-8,9-dihydroxy-10a,12a-dimethyl-6H-benz[c]indeno[5,4-e]oxepin-6-one

(3aS,5S,6R,7aR,7bS,9aS,10R,12aS,12bS)-10-[(2S,3R,4R,5R)-3,4-Dihydroxy-5,6-dimethyl-2-heptanyl]-5,6-dihydroxy-7a,9a-dimethylhexadecahydro-3H-benzo[c]indeno[5,4-e]oxepin-3-one

(2a,3a,5a,22R,23R,24S)-2,3,22,23-Tetrahydroxy-B-homo-7-oxaergostan-6-one

Brassinolide

(3aS,5S,6R,7aR,7bS,9aS,10R,12aS,12bS)-10-[(2S,3R,4R,5S)-3,4-Dihydroxy-5,6-dimethylheptan-2-yl]-5,6-dihydroxy-7a,9a-dimethylhexadecahydro-3H-benzo[c]indeno[5,4-e]oxepin-3-one

brassinosteroid

MFCD00133310

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