CAS 105637-50-1|ML-9

Introduction:Basic information about CAS 105637-50-1|ML-9, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
Common NameML-9
CAS Number105637-50-1Molecular Weight361.287
Density/Boiling Point508.8ºC at 760mmHg
Molecular FormulaC15H18Cl2N2O2SMelting Point196-200ºC
MSDSUSAFlash Point261.5ºC

Names

Name1-(5-Chloronaphthalenesulfonyl)-1H-hexahydro-1,4-diazepine, Hydrochloride
SynonymMore Synonyms

ML-9 BiologicalActivity

DescriptionML-9 is a selective and potent inhibitor of Akt kinase, inhibits myosin light-chain kinase (MLCK) and stromal interaction molecule 1 (STIM1) activity[3]. ML-9 inhibits inhibits MLCK, PKA and PKC activity with Ki values of 4, 32 and 54 μM, respectively[1]. ML-9 induces autophagy by stimulating autophagosome formation and inhibiting their degradation[3].
Related CatalogResearch Areas >>CancerSignaling Pathways >>Cytoskeleton >>Myosin
In VitroML9 (0-100 μM; 0-24 hours) has no reduction in cardiomyocyte viability, 50-100 μM significantly induces cell death[2]. ML9 (50 μM; 1-4 hours) significantly increases cleaved caspase-3 levels, decreased STIM1 protein levels by about 42%[2]. Cell Viability Assay[1] Cell Line: Neonatal rat ventricular myocytes (NRVM) cells Concentration: 0, 10, 50 and 100 μM Incubation Time: 0, 1, 4, 8 and 24 hours Result: Decreased cell viability at 50-100 μM concentration. Apoptosis Analysis[1] Cell Line: Neonatal rat ventricular myocytes (NRVM) cells Concentration: 50 μM Incubation Time: 1, 4 and 8 hours Result: Induced cardiomyocyte death through necrosis and apoptosis.
References

[1]. Ito S, et al. ML-9, a myosin light chain kinase inhibitor, reduces intracellular Ca2+ concentration in guinea pig trachealis.Eur J Pharmacol. 2004 Feb 23;486(3):325-33.

[2]. Shaikh S, et al. The STIM1 inhibitor ML9 disrupts basal autophagy in cardiomyocytes by decreasing lysosome content.Toxicol In Vitro. 2018 Apr;48:121-127.

[3]. Kondratskyi A1, et al.Identification of ML-9 as a lysosomotropic agent targeting autophagy and cell death.Cell Death Dis. 2014 Apr 24;5:e1193.

Chemical & Physical Properties

Boiling Point508.8ºC at 760mmHg
Melting Point196-200ºC
Molecular FormulaC15H18Cl2N2O2S
Molecular Weight361.287
Flash Point261.5ºC
Exact Mass360.046600
PSA57.79000
LogP4.62670
Vapour Pressure1.79E-10mmHg at 25°C
InChIKeyZNRYCIVTNLZOGI-UHFFFAOYSA-N
SMILESCl.O=S(=O)(c1cccc2c(Cl)cccc12)N1CCCNCC1

Safety Information

Personal Protective EquipmentEyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
Hazard CodesXi
RIDADRNONH for all modes of transport
HS Code2933990090

Customs

HS Code2933990090
Summary2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

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Synonyms

1H-1,4-Diazepine, 1-[(5-chloro-1-naphthalenyl)sulfonyl]hexahydro-, hydrochloride (1:1)
1-[(5-Chloro-1-naphthyl)sulfonyl]-1,4-diazepane hydrochloride (1:1)
1-(5-chloronaphthalen-1-yl)sulfonyl-1,4-diazepane,hydrochloride
MFCD00065525
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