CAS 91396-88-2|PluriSln 1

Introduction：Basic information about CAS 91396-88-2|PluriSln 1, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Table of Contents

1. Names
2. PluriSln 1 BiologicalActivity
3. Chemical & Physical Properties
4. Safety Information
5. Synonyms

Common Name	PluriSln 1
CAS Number	91396-88-2	Molecular Weight	213.235
Density	1.3±0.1 g/cm3	Boiling Point	335.7±15.0 °C at 760 mmHg
Molecular Formula	C₁₂H₁₁N₃O	Melting Point	177-178℃
MSDS	ChineseUSA	Flash Point	156.9±20.4 °C
Symbol	GHS07	Signal Word	Warning

Names

Name	N'-phenylpyridine-4-carbohydrazide
Synonym	More Synonyms

PluriSln 1 BiologicalActivity

Description	PluriSln 1 is an inhibitor of stearoyl-coA desaturase (SCD), and is a pluripotent cell-specific inhibitor.
Related Catalog	Signaling Pathways >>Metabolic Enzyme/Protease >>Stearoyl-CoA Desaturase (SCD)Research Areas >>Cancer
Target	SCD[1]
In Vitro	PluriSln 1, a small-molecule inhibitor of stearoyl-coA desaturase (SCD), on induced pluripotent stem cells (iPS)-derived cardiomyocytes (CM). PluriSln 1 treatment significantly decreases the mRNA and protein level of Nanog, a marker for both cell pluripotency and tumor progression; importantly, we provide evidence that PluriSln 1 treatment at 20 µM for 1 day significantly induces the apoptosis of Nanog-positive iPS derivates (iPSD). In addition, PluriSln 1 treatment at 20 µM for 4 days diminished Nanog-positive stem cells in cultured iPSD while not increasing apoptosis of iPS-derived CM. To investigate whether PluriSln 1 treatment prevents tumorigenicity of iPSD after cell transplantation, we intramyocardially injected PluriSln 1- or DMSO-treated iPSD in a mouse model of myocardial infarction (MI). DMSO-treated iPSD readily formed Nanog-expressing tumors 2 weeks after injection, which is prevented by treatment with PluriSln 1. Moreover, treatment with PluriSln 1 does not change the expression of cTnI, α-MHC, or MLC-2v, markers of cardiac differentiation (P>0.05, n=4). Importantly, PluriSln 1-treated iPS-derived CM exhibits the ability to engraft and survive in the infarcted myocardium[1].
Cell Assay	The differentiation of iPS cells to cardiomyocytes (CM) is induced by embryoid body (EB) formation. When iPS cells reached 70% confluency in 10-cm dishes, cells are digested using 0.25% trypsin/EDTA. Cell pellets are re-suspended in differentiation medium (DMEM with 20% FBS and 10 ng/mL BMP4) to a final concentration of 200,000 cells/mL. Cell suspensions are added to 6-well plates with Ulta-Low Attachment surfaces for 4 d to initiate EB formation. On day 5, EBs are cultured on 0.1% gelatin-coated dishes for 14 d using CF culture medium for the outgrowth of cardiac structures. At this stage, iPS cells undergoing EB formation are termed iPS derivates (iPSD)[1].
References	[1]. Zhang L, et al. Inhibition of stearoyl-coA desaturase selectively eliminates tumorigenic Nanog-positive cells: improving the safety of iPS cell transplantation to myocardium. Cell Cycle. 2014;13(5):762-71.

Chemical & Physical Properties

Density	1.3±0.1 g/cm3
Boiling Point	335.7±15.0 °C at 760 mmHg
Melting Point	177-178℃
Molecular Formula	C₁₂H₁₁N₃O
Molecular Weight	213.235
Flash Point	156.9±20.4 °C
Exact Mass	213.090210
PSA	54.02000
LogP	1.34
Appearance of Characters	white to beige
Vapour Pressure	0.0±0.7 mmHg at 25°C
Index of Refraction	1.655
InChIKey	HUDWXDLBWRHCKO-UHFFFAOYSA-N
SMILES	O=C(NNc1ccccc1)c1ccncc1
Storage condition	?20°C
Water Solubility	DMSO: soluble15mg/mL, clear

Safety Information

Symbol	GHS07
Signal Word	Warning
Hazard Statements	H302-H319
Precautionary Statements	P305 + P351 + P338
Hazard Codes	Xn
Risk Phrases	22-36
Safety Phrases	26
RIDADR	NONH for all modes of transport

Synonyms

1-Isonicotinoyl-2-phenyl-hydrazin

PluriSln 1

N'-Phenylisonicotinohydrazide

Isonicotinsaeure-phenylhydrazid

Isonicotinsaeure-(2-phenyl-hydrazid)

4-Pyridinecarboxylic acid, 2-phenylhydrazide

Isonicotinsaeure-(N'-phenyl-hydrazid)

isonicotinic acid N'-phenylhydrazide

NSC 14613

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