CAS 27072-45-3|fluorescein 5-isothiocyanate
| Common Name | fluorescein 5-isothiocyanate | ||
|---|---|---|---|
| CAS Number | 27072-45-3 | Molecular Weight | 389.381 |
| Density | 1.5±0.1 g/cm3 | Boiling Point | 708.6±60.0 °C at 760 mmHg |
| Molecular Formula | C21H11NO5S | Melting Point | >360ºC(lit.) |
| MSDS | ChineseUSA | Flash Point | 382.4±32.9 °C |
| Symbol | GHS08 | Signal Word | Danger |
Names
| Name | Fluorescein isothiocyanate |
|---|---|
| Synonym | More Synonyms |
fluorescein 5-isothiocyanate BiologicalActivity
| Description | 5(6)-FITC (Fluorescein isothiocyanate) is an amine-reactive derivative of fluorescein dye that has wide-ranging applications as a label for antibodies and other probes, for use in fluorescence microscopy, flow cytometry and immunofluorescence-based assays such as Western blotting and ELISA. |
|---|---|
| Related Catalog | Signaling Pathways >>Others >>OthersDye ReagentsResearch Areas >>Others |
| References | [1]. Goding JW. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Methods. 1976;13(3-4):215-26. [2]. Der-Balian GP, et al. Fluorescein labeling of Fab' while preserving single thiol. Anal Biochem. 1988 Aug 15;173(1):59-63. [3]. Smith LM, et al. Fluorescence detection in automated DNA sequence analysis. Nature. 1986 Jun 12-18;321(6071):674-9. |
Chemical & Physical Properties
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 708.6±60.0 °C at 760 mmHg |
| Melting Point | >360ºC(lit.) |
| Molecular Formula | C21H11NO5S |
| Molecular Weight | 389.381 |
| Flash Point | 382.4±32.9 °C |
| Exact Mass | 389.035797 |
| PSA | 120.44000 |
| LogP | 4.00 |
| Appearance of Characters | powder |
| Vapour Pressure | 0.0±2.3 mmHg at 25°C |
| Index of Refraction | 1.754 |
| Storage condition | 2-8°C |
Safety Information
| Symbol | GHS08 |
|---|---|
| Signal Word | Danger |
| Hazard Statements | H334 |
| Precautionary Statements | P261-P342 + P311 |
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Faceshields;Gloves |
| Hazard Codes | Xn: Harmful; |
| Risk Phrases | R42/43 |
| Safety Phrases | S22 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | 3 |
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Synonyms
| 5-FITC |
| Isothiocyanatofluorescein |
| 5-Isothionatofluorescein |
| FITC isomer I |
| Fluorescein Isothiosyanate |
| fluorescein isothiocyanate isomer 1 |
| FITC ISOMER |
| EINECS 222-042-0 |
| 3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[2-benzofurane-1,9'-xanthén]-3-one |
| Spiro(isobenzofuran-1(3H),9'-(9H)xanthen)-3-one, 3',6'-dihydroxy-5-isothiocyanato- |
| FITC-I |
| Fluorescein 5(6)-isothiocyanate |
| FITC |
| Fluorescein-5-isothiocyanate |
| MFCD00005063 |
| Spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, 3',6'-dihydroxy-5-isothiocyanato- |
| Fluorescein Isothiocyanate |
| fluorescein 5-isothiocyanate |
| 3',6'-Dihydroxy-5-isothiocyanato-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one |
| 5(6)-FITC |
