CAS 23491-52-3|Hoechst 33342

Introduction：Basic information about CAS 23491-52-3|Hoechst 33342, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Table of Contents

1. Names
2. Hoechst 33342 BiologicalActivity
3. Chemical & Physical Properties
4. Toxicological Information
CHEMICAL IDENTIFICATION
HEALTH HAZARD DATA
ACUTE TOXICITY DATA
MUTATION DATA
5. Safety Information
6. Customs
7. Articles72
8. Synonyms

Common Name	Hoechst 33342
CAS Number	23491-52-3	Molecular Weight	452.55
Density	/	Boiling Point	/
Molecular Formula	C₂₇H₂₈N₆O	Melting Point	268ºC
MSDS	ChineseUSA	Flash Point	/
Symbol	GHS07, GHS08	Signal Word	Warning

Names

Name	2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole
Synonym	More Synonyms

Hoechst 33342 BiologicalActivity

Description	Hoechst 33342 is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.
Related Catalog	Signaling Pathways >>Autophagy >>AutophagyDye ReagentsResearch Areas >>Others
Target	Dye reagent[1] DNA Stain[1]
In Vitro	Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1].
Cell Assay	Labeling Nuclear DNA with Hoechst 33342[1]Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+.Step 5, Mount the coverslips.Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1].
References	[1]. Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.

Chemical & Physical Properties

Melting Point	268ºC
Molecular Formula	C₂₇H₂₈N₆O
Molecular Weight	452.55
PSA	100.76000
LogP	7.13970
InChIKey	PRDFBSVERLRRMY-UHFFFAOYSA-N
SMILES	CCOc1ccc(-c2nc3ccc(-c4nc5ccc(N6CCN(C)CC6)cc5[nH]4)cc3[nH]2)cc1
Storage condition	2-8°C

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: DT4200000

CHEMICAL NAME :: 2,5'-Bibenzimidazole, 2'-(p-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-

CAS REGISTRY NUMBER :: 23491-52-3

LAST UPDATED :: 199312

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

MUTATION DATA

TYPE OF TEST :: DNA inhibition

TEST SYSTEM :: Rodent - hamster Lung

DOSE/DURATION :: 1 umol/L

REFERENCE :: JHCYAS Journal of Histochemistry and Cytochemistry. (Elsevier Science Pub. Co., Inc., 52 Vanderbilt Ave., New York, NY 10017) V.1- 1953- Volume(issue)/page/year: 30,111,1982

Safety Information

Symbol	GHS07, GHS08
Signal Word	Warning
Hazard Statements	H302-H315-H335-H341
Precautionary Statements	P280-P301 + P312 + P330
Personal Protective Equipment	dust mask type N95 (US);Eyeshields;Gloves
Hazard Codes	Xn: Harmful;
Risk Phrases	R22
Safety Phrases	S22-S36/37-S24/25
RIDADR	NONH for all modes of transport
WGK Germany	3
RTECS	SM1140500
HS Code	2933990090

Customs

HS Code	2933990090
Summary	2933990090. heterocyclic compounds with nitrogen hetero-atom(s) only. VAT:17.0%. Tax rebate rate:13.0%. . MFN tariff:6.5%. General tariff:20.0%

Articles72

Comparative in vitro study on magnetic iron oxide nanoparticles for MRI tracking of adipose tissue-derived progenitor cells.

PLoS ONE 9(9) , e108055, (2014)

Magnetic resonance imaging (MRI) using measurement of the transverse relaxation time (R2*) is to be considered as a promising approach for cell tracking experiments to evaluate the fate of transplante...

DOCK8 regulates lymphocyte shape integrity for skin antiviral immunity.

J. Exp. Med. 211(13) , 2549-66, (2014)

DOCK8 mutations result in an inherited combined immunodeficiency characterized by increased susceptibility to skin and other infections. We show that when DOCK8-deficient T and NK cells migrate throug...

Unique Features of Human Protein Arginine Methyltransferase 9 (PRMT9) and Its Substrate RNA Splicing Factor SF3B2.

J. Biol. Chem. 290 , 16723-43, (2015)

Human protein arginine methyltransferase (PRMT) 9 symmetrically dimethylates arginine residues on splicing factor SF3B2 (SAP145) and has been functionally linked to the regulation of alternative splic...

Synonyms

bisBenzimide H 33342 trihydrochloride

2,5'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-, trihydrochloride

2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzo[d]imidazole

2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-1H,1'H-2,5'-bibenzimidazole trihydrochloride

2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole

2,6'-Bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-6-(4-methyl-1-piperazinyl)-, hydrochloride (1:3)

MFCD00166996

BISBENZIMIDE ETHOXIDE TRIHYDROCHLORIDE

2,5'-bi-1H-benzimidazole, 2'-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-, hydrochloride (1:3)

Hoe 33342

2'-(4-Ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-1H,1'H-2,5'-bibenzimidazole trihydrochloride

EINECS 245-691-1

Hoechst 33342

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