CAS 117464-70-7|BCECF-AM
Introduction：Basic information about CAS 117464-70-7|BCECF-AM, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
| Common Name | BCECF-AM | ||
|---|---|---|---|
| CAS Number | 117464-70-7 | Molecular Weight | 820.70 |
| Density | 1.5±0.1 g/cm3 | Boiling Point | 939.2±65.0 °C at 760 mmHg |
| Molecular Formula | C40H36O19 | Melting Point | / |
| MSDS | USA | Flash Point | 368.9±34.3 °C |
Names
| Name | 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymet |
|---|---|
| Synonym | More Synonyms |
BCECF-AM BiologicalActivity
| Description | BCECF-AM is a cell membrane permeable compound, widely used as a fluorescent indicator for intracellular pH. |
|---|---|
| Related Catalog | Signaling Pathways >>Others >>OthersDye ReagentsResearch Areas >>Others |
| In Vitro | BCECF-AM is used to measure changes in basal pHi and NHE activity induced by increasing concentrations of ET-1 (0.1-10 nM) in pulmonary arterial smooth muscle cell (PASMC)[1]. |
| Cell Assay | PASMCs are placed in a laminar flow cell chamber perfused with HBSS with pH adjusted to 7.4. pHi is measured in cells incubated with the membrane permeant (acetoxymethyl ester) form of the pH-sensitive fluorescent dye BCECF-AM for 60 min at 37°C under an atmosphere of 20% O2-5% CO2. Cells are then washed with HBSS for 15 min at 37°C to remove extracellular dye and allow complete de-esterification of cytosolic dye. Ratiometric measurement of BCECF fluorescence is performed on a workstation consisting of a Nikon TSE 100 Ellipse inverted microscope with epi-fluorescence attachments. The light beam from a xenon arc lamp is filtered by interference filters at 490 and 440 nm, and focused onto the PASMCS under examination via a 20× fluorescence objective. Light emitted from the cell at 530 nm is returned through the objective and detected by an imaging camera. An electronic shutter is used to minimize photobleaching of dye. Protocols are executed and data collected on-line with InCyte software. pHi is estimated from in situcalibration after each experiment. Cells are perfused with a solution containing (in mM): 105 KCl, 1 MgCl2, 1.5 CaCl2, 10 glucose, 20 HEPES-Tris and 0.01 nigericin to allow pHi to equilibrate to external pH. A two point calibration is created from fluorescence measured as pHi is adjusted with KOH from 6.5 to 7.5. Intracellular H+ ion concentration ([H+]i) is determined from pHi using the formula: pHi = −log ([H+]i). |
| References | [1]. Clark Undem, et al. Endothelin-1 Augments Na+/H+ Exchange Activity in Murine Pulmonary Arterial Smooth Muscle Cells via Rho Kinase. PLoS One. 2012; 7(9): e46303. |
Chemical & Physical Properties
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 939.2±65.0 °C at 760 mmHg |
| Molecular Formula | C40H36O19 |
| Molecular Weight | 820.70 |
| Flash Point | 368.9±34.3 °C |
| PSA | 264.39000 |
| LogP | 0.90 |
| Vapour Pressure | 0.0±0.3 mmHg at 25°C |
| Index of Refraction | 1.602 |
| Storage condition | -20°C |
Safety Information
| Personal Protective Equipment | dust mask type N95 (US);Eyeshields;Gloves |
|---|---|
| Hazard Codes | Xi: Irritant; |
| Risk Phrases | R36/37/38 |
| Safety Phrases | S22-S26-S36 |
| RIDADR | NONH for all modes of transport |
Articles27
More Articles| Fast loading ester fluorescent Ca2+ and pH indicators into pollen of Pyrus pyrifolia. J. Plant Res. 125(1) , 185-95, (2012) Loading of Ca(2+)-sensitive fluorescent probes into plant cells is an essential step to measure activities of free Ca(2+) ions in cytoplasm with a fluorescent imaging technique. Fluo-3 is one of the m... | |
| Role of the plasma membrane calcium adenosine triphosphatase on domoate-induced intracellular acidification in primary cultures of cerebelar granule cells. J. Neurosci. Res. 84(2) , 326-37, (2006) Changes in intracellular pH (pH(i)) and cytosolic calcium concentration ([Ca(2+)](c)) caused by the glutamate agonist domoate (DOM) were studied in single cultured mouse cerebellar granule cells (CGC)... | |
| Platelet-activating factor stimulates cytoplasmic alkalinization and granule acidification in human eosinophils. J. Cell Sci. 117(Pt 24) , 5749-57, (2004) The effects of platelet-activating factor (PAF) and IL-5 on intracellular pH were investigated in human eosinophils. Purified peripheral blood eosinophils were loaded with the ratiometric fluorescent ... |
Synonyms
| Spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoic acid, 3',6'-bis[(acetyloxy)methoxy]-5-[[(acetyloxy)methoxy]carbonyl]-3-oxo-, bis[(acetyloxy)methyl] ester |
| MFCD00036969 |
| 2',7'-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein tetrakis(acetoxymethyl) ester |
| Acetoxymethyl 3',6'-bis(acetoxymethoxy)-2',7'-bis[3-(acetoxymethoxy)-3-oxopropyl]-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylate |
| 2′,7′-bis(2-Carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester Mixed isomers |
| acetyloxymethyl 3',6'-bis(acetyloxymethoxy)-2',7'-bis[3-(acetyloxymethoxy)-3-oxopropyl]-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate |
| BCECF-acetoxymethyl |
| BCECF-AM |
