CAS 305834-79-1|SC79

Introduction：Basic information about CAS 305834-79-1|SC79, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Table of Contents

1. Names
2. SC79 BiologicalActivity
3. Chemical & Physical Properties
4. Safety Information
5. Synonyms

Common Name	SC79
CAS Number	305834-79-1	Molecular Weight	364.780
Density	1.3±0.1 g/cm3	Boiling Point	524.8±50.0 °C at 760 mmHg
Molecular Formula	C₁₇H₁₇ClN₂O₅	Melting Point	/
MSDS	USA	Flash Point	271.2±30.1 °C

Names

Name	2-Amino-6-chloro-alpha-cyano-3-(ethoxycarbonyl)-4H-1-benzopyran-4-acetic acid ethyl ester
Synonym	More Synonyms

SC79 BiologicalActivity

Description	SC79 is a selective and cell-permeable Akt activator which activates Akt phosphorylation and inhibits Akt membrane translocation.
Related Catalog	Signaling Pathways >>PI3K/Akt/mTOR >>AktResearch Areas >>Cancer
Target	Akt
In Vitro	SC79 reduces neuronal excitotoxicity and prevents stroke-induced neuronal death. SC79 suppresses PHAKTM-GFP plasma membrane translocation, and enhances phosphorylation of all three Akt isoforms in HEK293, HeLa, HL60, NB4, and HsSulton (B cells) cells[1]. SC79 restores proliferation of BRAT1 knockdown cells, and reduces the production of superoxide in mitochondria of MitoSox positive cells[2].
In Vivo	SC79 (0.04 mg/g, i.p.) inhibits the cytosolic activation of Akt, and recapitulates the primary cellular function of Akt signaling, resulting in augmented neuronal survival, in the permanent focal cerebral ischemia mouse model[1].
Cell Assay	HsSultan or NB4 cells (2.5×105) are plated in a 24-well plate in 500 μL of phenol red-free RPMI medium supplemented with 10% FBS. After incubation for 24 hours, each compound (8 µg/mL) is added and cultured for overnight (16-20 h). Fifty μLs of MTT solution (5 mg/mL in PBS) are added to each well. Following 2 hrs incubation, the purple formazan crystals are dissolved by directly adding in 500 μL of isopropanol with 0.1mol/LHCl to each well. After clearing the cell debris by centrifugation, the absorbance is measured at a wavelength of 570 nm.
Animal Admin	The permanent focal cerebral ischemia is induced by middle cerebral artery occlusion (MCAO) essentially. Briefly, mice (C57 Black/6) weighing 17-25 g are anesthetized with 4% isoflurane/66% N2O/30% O2 and maintained with 1.5% isoflurane. Permanent focal ischemia is achieved as follows: a 2-mm hole is drilled at a site superior and lateral to the left foramen ovale to expose the left middle cerebral artery. The proximal portion of the left middle cerebral artery (MCA) is permanently occluded over a 1-mm segment distal to the origin of the lenticulostriate branches through the use of a bipolar coagulator. SC79 is injected intraperitoneally (0.04 mg/g mouse body weight) 5 min before permanent MCAO. In another experiment, extra SC79 is injected (0.04 mg/g mouse body weight, once per hour for 6 hours).
References	[1]. Jo H, et al. Small molecule-induced cytosolic activation of protein kinase Akt rescues ischemia-elicited neuronal death. Proc Natl Acad Sci U S A. 2012 Jun 26;109(26):10581-10586. [2]. So EY, et al. BRAT1 deficiency causes increased glucose metabolism and mitochondrial malfunction. BMC Cancer. 2014 Jul 29;14:548

Chemical & Physical Properties

Density	1.3±0.1 g/cm3
Boiling Point	524.8±50.0 °C at 760 mmHg
Molecular Formula	C₁₇H₁₇ClN₂O₅
Molecular Weight	364.780
Flash Point	271.2±30.1 °C
Exact Mass	364.082611
LogP	3.23
Vapour Pressure	0.0±1.4 mmHg at 25°C
Index of Refraction	1.564
InChIKey	DXVKFBGVVRSOLI-UHFFFAOYSA-N
SMILES	CCOC(=O)C1=C(N)Oc2ccc(Cl)cc2C1C(C#N)C(=O)OCC
Storage condition	-20℃

Safety Information

RIDADR	NONH for all modes of transport

Synonyms

Ethyl 2-amino-6-chloro-4-(1-cyano-2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate

4H-1-Benzopyran-4-acetic acid, 2-amino-6-chloro-α-cyano-3-(ethoxycarbonyl)-, ethyl ester

SC-79

SC79

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