Introduction：Basic information about 5,5′-Dithiobis(2-nitrobenzoic acid) CAS 69-78-3, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
5,5′-Dithiobis(2-nitrobenzoic acid) Basic information
| Product Name: | 5,5′-Dithiobis(2-nitrobenzoic acid) |
| Synonyms: | DTNB;ellmann's reagent;ELLMAN'S REAGENT;ELLMANS' REAGENT;BIS(3-CARBOXY-4-NITROPHENYL) DISULFIDE;3-CARBOXY-4-NITROPHENYL DISULFIDE;Ellmsn'sreagent;Bis(3-carboxy-4-nitrophenyl) disulphide~3-Carboxy-4-nitrophenyl disulphide~Ellmans |
| CAS: | 69-78-3 |
| MF: | C14H8N2O8S2 |
| MW: | 396.35 |
| EINECS: | 200-714-4 |
| Product Categories: | marker;Phenyls & Phenyl-Het;Phenyls & Phenyl-Het |
| Mol File: | 69-78-3.mol |
|
5,5′-Dithiobis(2-nitrobenzoic acid) Chemical Properties
| Melting point | 240-245 °C (dec.)(lit.) |
| Boiling point | 671.9±55.0 °C(Predicted) |
| bulk density | 290kg/m3 |
| density | 1.67 |
| refractive index | 1.6900 (estimate) |
| storage temp. | Keep in dark place,Sealed in dry,Room Temperature |
| solubility | ethanol: 8mg/mL |
| pka | 1.57±0.25(Predicted) |
| form | Yellow solid |
| color | Pale Yellow to Light Yellow |
| Water Solubility | Soluble in water and ethanol. |
| Merck | 14,3551 |
| BRN | 1896821 |
| Stability: | Stable. Incompatible with strong bases, strong oxidizing agents. |
| InChI | 1S/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20) |
| InChIKey | KIUMMUBSPKGMOY-UHFFFAOYSA-N |
| SMILES | OC(=O)c1cc(SSc2ccc(c(c2)C(O)=O)[N+]([O-])=O)ccc1[N+]([O-])=O |
| CAS DataBase Reference | 69-78-3(CAS DataBase Reference) |
| EPA Substance Registry System | Benzoic acid, 3,3'-dithiobis[6-nitro- (69-78-3) |
Safety Information
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26-36 |
| WGK Germany | 3 |
| RTECS | DG9650000 |
| F | 10 |
| TSCA | TSCA listed |
| HS Code | 29309070 |
| Storage Class | 11 - Combustible Solids |
5,5′-Dithiobis(2-nitrobenzoic acid) Usage And Synthesis
| Chemical Properties | Crystalline |
| Uses | 5,5′-Dithiobis(2-nitrobenzoic acid) is an membrane-impermeant thiol/disulfide exchange blocker. It is also used to quantify the number or concentration of thiol groups in a sample. |
| Uses | Reagent for determination of sufhydryl groups.5,5'-dithiobis-(2-nitrobenzoic acid) is widely utilized as a non-fluorescent probe to quantify the number or the concentration of thiol groups in a sample. It acts as a cleavable cross-linking agent. It finds an important application as a sensitive reagent for determining free sulfhydryl content in proteins, peptides, and tissues. It is also employed in high-performance liquid chromatography (HPLC) to determine the alkylthiol content. |
| Uses | 5,5′-Dithiobis(2-nitrobenzoic acid) is a water soluble reagent mainly used for the derivatization of sulfhydryl groups. |
| Definition | ChEBI: An organic disulfide that results from the formal oxidative dimerisation of 2-nitro-5-thiobenzoic acid. An indicator used to quantify the number or concentration of thiol groups. |
| General Description | 5,5′-Dithiobis(2-nitrobenzoic acid) is also called DTNB or Ellman′s reagent. It is a water soluble reagent, which interacts with thiol groups, and can be used conveniently at neutral pH. Thiolate anion (RS-) reacts with Ellman′s reagent (DTNB2-), to produce 2-nitro-5-thiobenzoate anion (TNB2-) and one mixed disulfide (R-S-TNB-). TNB2- has a high molar absorption coefficient, and absorbs at 412nm. This absorption is independent of pH, at pH> 7.3. |
| Biological Activity | Cell permeable: no', 'Primary Target
Useful in photometric determination of thiols', 'Product does not compete with ATP.', 'Reversible: no |
5,5′-Dithiobis(2-nitrobenzoic acid) Preparation Products And Raw materials
