Anistreplasa [INN-Spanish] CAS 81669-57-0

Introduction：Basic information about Anistreplasa [INN-Spanish] CAS 81669-57-0, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

Anistreplasa [INN-Spanish] Basic information

• Product Name: Anistreplasa [INN-Spanish]
• Synonyms: Anistreplasa [INN-Spanish];Anistreplase;APSAC;BRL 26921;Eminase;Anistreplasa [INN-Spanish] USP/EP/BP
• CAS: 81669-57-0
• MW: 0
• Mol File: Mol File

Anistreplasa [INN-Spanish] Usage And Synthesis

Description

APSAC is an anisoylated human plasminogen-streptokinase complex useful in thetreatment of myocardial infarct. Its reported advantages over other existing thrombolyticssuch as alteplase and streptokinase include a longer half life, ease of administration, and amore specific local antithrombotic effect.

Originator

Beecham (United Kingdom)

Uses

Fibrinolytic.

Manufacturing Process

Preparation of freeze dried p-anisoyl streptokinase/plasminogen complexwithout internal peptide bond cleavages:
Streptokinase (250,000 units, Kabi, Stockholm, Sweden) was dissolved in 0.1M trishydroxymethylmethane hydrochloride pH 7.4 (2.5 ml) and a solution of0.1 M p-amidinophenyl p'-anisate in dimethylsulphoxide (0.25 ml) added. Aslight cloudiness resulted. To this mixture was added 0.5 ml of a solution ofhuman lys-plasminogen (8.99 mg/ml in the above buffer) [Kabi, Stockholm]and the solution was thoroughly and rapidly mixed. After standing on ice for15 minutes, the solution was stored on ice until use. After approximaely 2hours 0.4 ml (40,000 units) of the material was diluted with 3.0 ml of a slurryof L-lysine-sepharose 4B (a 33% wet wt/volume suspension in the abovebuffer) and stood at 0°C for 1 hour. The gel was filtered on a glass sinter funnel at 4°C under suction and washed with the buffer (100 ml). The gel waseluted under gentle suction with the same buffer containing 0.1 M ε-aminocaproic acid (3 lots of 5 ml). The combined filtrates were dialysed for 2hours at 4°C against ammonium bicarbonate buffer (50 mM pH 7.0)containing 2.5% w/v mannitol. The product was then freeze dried to 0.799 gof a white solid. Approximately 195 mg of this material was analysed by slabgel polyacrylamide gel electrophoresis using 10% w/v gels in the presence ofsodium dodecyl sulphate. Two main polypeptide bands were observedcorresponding to lys-plasminogen (m.w. 84,000) and streptokinase (m.w.47,000). There was also a trace of contaminating human serum albuminderived from the commercial streptokinase preparation.

Brand name

Eminase

Therapeutic Function

Thrombolytic