APS-5 CAS 193884-53-6

Introduction：Basic information about APS-5 CAS 193884-53-6, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

APS-5 Basic information

• Product Name: APS-5
• Synonyms: [(4-Chlorophenyl)thio](10-methyl-9(10H)-acridinylidene)-methanol 1-(dihydrogen phosphate) disodium salt (1:2);Lumigen APS 5;APS-5;Disodium [(4-chlorophenyl)sulfanyl](10-methyl-9(10H)-acridinylidene)methyl phosphate;9-(4-Chlorophenylthiophosphoryloxymethylidene)-10-methylacridan,disodium salt;[(4-chlorophenyl)thio](10-methyl-9(10H)-acridinylidene)methanol 1-(dihydrogen phosphate) disodium salt;APS-5，9-(4-Chlorophenylthiophosphoryloxymethylidene)-10-methylacrid;Methanol, [(4-chlorophenyl)thio](10-methyl-9(10H)-acridinylidene)-, 1-(dihydrogen phosphate), disodium salt (1:2)
• CAS: 193884-53-6
• MF: C21H18ClNNaO4PS
• MW: 469.85
• Mol File: 193884-53-6.mol

APS-5 Chemical Properties

• form: Solid
• color: White to light yellow
• InChI: InChI=1S/C21H17ClNO4PS.Na.H/c1-23-18-8-4-2-6-16(18)20(17-7-3-5-9-19(17)23)21(27-28(24,25)26)29-15-12-10-14(22)11-13-15;;/h2-13H,1H3,(H2,24,25,26);;
• InChIKey: NPDSHDRLAYHQFC-UHFFFAOYSA-N
• SMILES: C(=C1C2=CC=CC=C2N(C)C2C=CC=CC1=2)(OP(O)(O)=O)SC1C=CC(Cl)=CC=1.[NaH]

APS-5 Usage And Synthesis

Description

Lumigen APS-5 is a proprietary acridan based chemiluminescentsubstrate for ELISA detection of alkaline phosphatase (AP)conjugated molecules. The unique acridan chemistry of LumigenAPS-5 provides temperature insensitive light output and morerapid peak intensity as compared to the dioxetane based APsubstrates, Lumi-Phos 530 and Lumi-Phos Plus.

Uses

Aps-5 is a chemiluminescent substrate based on 9, 10-dihydroacridine, which is mainly used for ELISA detection of compounds with alkaline phosphatase AP conjugation.The 9, 10-dihydroacridine structure in the aps-5 structure can continuously, stably and efficiently give off light under the action of AP, and aps-5 is very suitable for the determination of phosphatase activity solution and phosphatase-linked immunoassay.

Mechanism of action

Lumigen APS-5 utilizes a unique technology for the chemiluminescentdetection of alkaline phosphatase conjugates which combine excellentsensitivity with ease of use. Reaction of the acridan substrate with anAP label rapidly produces sustained high-intensity chemiluminescence.Lumigen APS-5 is ideally suited for solution assays of phosphataseactivity and for phosphatase-linked immunoassays.

Advantages

1.High sensitivity -- protein markers less than 5pg can be detected;2. High luminescence intensity -- peak luminescence can be achieved in a short time;3. Continuous and stable luminescence - the luminescence process is not affected by temperature within 25-35 days and does not need temperature control.