CARBOXYPEPTIDASE B CAS 9025-24-5

Introduction：Basic information about CARBOXYPEPTIDASE B CAS 9025-24-5, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.

CARBOXYPEPTIDASE B Basic information

• Product Name: CARBOXYPEPTIDASE B
• Synonyms: PROTEIN CARBOXYPEPTIDASE B;PROTAMINASE;PEPTIDYL-L-LYSINE[L-ARGININE] HYDROLASE;CARBOXYPEPTIDASE B, PORCINE PANCREAS;EC 3.4.17.2;IUB: 3.4.17.2;carboxypeptidase B from hog pancreas;RecoMbinant Carboxypeptidase B
• CAS: 9025-24-5
• MF: C31H38N4O7S
• MW: 610.72102
• EINECS: 232-788-9
• Mol File: 9025-24-5.mol

CARBOXYPEPTIDASE B Chemical Properties

• storage temp.: -20°C
• solubility: water: 1 mg/mL
• form: powder
• color: white
• biological source: Porcine pancreas
• Water Solubility: water: 1mg/mL
• Specific Activity: 50-55units/mg protein carboxypeptidase B
• InChIKey: TWURVFFNODFJBJ-UHFFFAOYSA-N
• SMILES: C(O)(=O)CCC(NC(C)C(NC(C)C(N1CCCC1C(=O)NC(CC1=CC=CC=C1)C(SCC1=CC=CC=C1)=O)=O)=O)=O

Safety Information

• Hazard Codes: Xi,B,Xn
• Risk Statements: 36-42-36/37/38
• Safety Statements: 26-36-36/37-24-22
• WGK Germany: 3
• Storage Class: 10 - Combustible liquids
• Hazard Classifications: Eye Irrit. 2 Resp. Sens. 1 Skin Irrit. 2 STOT SE 3

CARBOXYPEPTIDASE B Usage And Synthesis

Uses

The enzyme from Sigma has been used to develop homogeneous time-resolved fluorescence (HTRF) assay for measuring carboxypeptidase B activity in a miniaturized high-throughput screening format. It has been used to evaluate the impact of the C-terminal lysine(s) in human plasminogen binding to Bifidobacterium. The effect of treatment with carboxypeptidase B, which is a C-terminal lysine-specific endopeptidase, is measured using flow cytometry analysis.

Uses

Carboxypeptidase B from Sigma has been used as a reference for assaying carboxypeptidase activity in lysed pituitary granules derived from the anterior and intermediate lobes of rat. The enzyme has also been used to digest plasma samples by removing C-terminal basic amino acids, to get a distinct band for each allotype during C4 electrophoresis.

General Description

Native carboxypeptidase B catalyzes the release of C-terminal from basic amino acids, arginine, lysine, or ornithine from polypeptides. Inhibited by EDTA and other Zn²⁺ chelators.

Biochem/physiol Actions

Carboxypeptidase B is a proteolytic enzyme capable of rapidly hydrolyzing peptide bonds to release certain carboxyl-terminal basic amino acids from peptides and proteins. Its molecular mass is 34,300±600 Da. It contains one non-dialyzable gram atom of zinc per mole. The enzyme activity is inhibited by metal chelating agents 1, 10-phenanthroline, 8-hydroxyquinoline-5-sulfonic acid, and 2,2′-dipyridyl.