Introduction：Basic information about Cy3 Methyltetrazine CAS 1676067-48-3, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
Cy3 Methyltetrazine Basic information
| Product Name: | Cy3 Methyltetrazine |
| Synonyms: | Cy3 Methyltetrazine;Trisulfo-Cy3 methyltetrazine;Cy3 Methyltetrazine (CCT-1018) |
| CAS: | 1676067-48-3 |
| MF: | C42H49N7O10S3 |
| MW: | 908.07 |
| EINECS: | |
| Product Categories: | |
| Mol File: | 1676067-48-3.mol |
|
Cy3 Methyltetrazine Chemical Properties
| solubility | Water, DMSO, DMF, MeOH |
| Appearance | Red solid |
Safety Information
Cy3 Methyltetrazine Usage And Synthesis
| Description | Methyltetrazine-activated Cy3 probe that reacts with TCO-containing compounds via an Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures. The inverse-electron demand Diels-Alder cycloaddition reaction of TCO with tetrazines is a bioorthogonal reaction that possesses exceptional kinetics (k > 800 M-1 -1) and selectivity. Such excellent reaction rate constants are unparalleled by any other bioorthogonal reaction pair described to date. described to date.Cy®3 is a bright, water-soluble, and pH insensitive orange-fluorescent dye that can be excited using the 532 nm or 555 nm laser line and visualized with TRITC (tetramethylrhodamine) filter sets. Cy3 conjugates give less background than TAMRA and most other commonly used fluorescent dyes. |
| Uses | Trisulfo-Cy3 Methyltetrazine is a click chemistry reagent containing anmethyltetrazine group. Methyltetrazine-activated Cy3 probe reacts with TCO-containing compounds via an Inverse-Electron-Demand Diels-Alder reaction to form a stable covalent bond and does not require Cu-catalyst or elevated temperatures[1][2]. |
| References | [1] Cy3 Methyltetrazine.
[2] Vera F C Ferreira, et al. In Vivo Pretargeting Based on Cysteine-Selective Antibody Modification with IEDDA Bioorthogonal Handles for Click Chemistry. Bioconjug Chem. 2021 Jan 20;32(1):121-132. |
| Abs/Em Maxima | 553/569 nm |
| Extinction Coefficient | 150,000 |
| Microscopy Laser Line | 532 or 555 nm |
| Flow Cytometry Laser Line | 532 or 555 nm |
| Spectrally Similar Dyes | Alexa Fluor® 555, Atto™ 555, CF™ 555 Dye, DyLight™549 |
Cy3 Methyltetrazine Preparation Products And Raw materials
