DISPASE CAS 9068-59-1
Introduction：Basic information about DISPASE CAS 9068-59-1, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
DISPASE Basic informationDescription Source
| Product Name: | DISPASE |
| Synonyms: | PROTEASE, NEUTRAL;PROTEASE, CALCIUM ACTIVATED;PRONASE E;NEUTRAL PROTEASE;Dispase, BR，50u/mg;NEUTRAL PROTEASE, GRADE II;NEUTRAL PROTEASE, GRADE I;STREPTOMYCES GRISEUS NEUTRAL PROTEINASE |
| CAS: | 9068-59-1 |
| MF: | |
| MW: | 0 |
| EINECS: | 232-966-6 |
| Product Categories: | |
| Mol File: | Mol File |
DISPASE Chemical Properties
| storage temp. | −20°C |
| form | lyophilized powder |
| color | Light yellow to brown |
| PH | 6-7 (20°C, 10g/L in H2O) |
| CAS DataBase Reference | 9068-59-1 |
Safety Information
| Hazard Codes | Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 22-24-26-36/37 |
| WGK Germany | 3 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Eye Irrit. 2 Resp. Sens. 1 Skin Irrit. 2 STOT SE 3 |
| Uses | Pronase E (from Streptomyces griseus) 4,000,000 PU/g for biochemistry EC 3.4.24.4. CAS 9036-06-0, pH 6?-?7 (10?g/l, H?O, 20?°C). |
| General Description | from Streptomyces griseus 4,000,000 PU/g for biochemistry EC 3.4.24.4 |
| Description | Neutral Protease (Dispase®) is isolated from Bacillus polymyxa and is a mild proteolytic enzyme that separates the epidermis from dermis by cleaving the basement membrane zone of the skin while preserving the viability of epithelial sheets. This enzyme is effective at cleaving fibronectin and type IV collagen in the lamina densa of skin. It will not cleave serum albumin, transferrin, laminin, type V collagen, or anchoring fibrils containing type VII collagen. As described in the expired U.S. Patent 4,304,866, Neutral Protease (Dispase®) is activated by metallic ions such as Ca++, Mn++, Mg++, Fe++, Fe+++, and Al+++ and is inhibited by metal-chelating agents such as ethylenediaminetetraacetic acid (EDTA), citric acid, o-phenanthroline, 2,2'-dipyridyl and sodium fluoride, and oxidizing agents such as N-bromosuccinimide (NBS) and iodine. Neutral Protease (Dispase®) is used at various concentrations for tissue dissociation, generally ranging from 0.6 to 2.4 U/ml for various time points depending on the desired effect. Concentrations higher than 2.4 U/ml are not recommended. |
| Source | B. polymyxa |
DISPASE Preparation Products And Raw materials
