Introduction：Basic information about EGS Crosslinker CAS 70539-42-3, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
EGS Crosslinker Basic information
| Product Name: | EGS Crosslinker |
| Synonyms: | ethylene glycol-bis(succinic acid n-hydroxysuccinimide;EGNHS;ethyleneglycolylbis(succinimidylsuccinate;ETHYLENE GLYCOL BIS-(SUCCINIC ACID*N-HYD ROXYSUCCINI;Ethlene glycol bis(succinimidyl succinate);Ethyleneglycol-O,O''-bis-(succinic acid N-hydroxysuccinimide ester);Di(N-succinimidyl) ethylene glycol disuccinate, EGS, Ethylene glycol bis(succinimidyl succinate), Ethylene glycol disuccinate di(N-succinimidyl) ester;Ethylene Glycol Bis(succinic Acid N-Hydrosuccinimide Ester) |
| CAS: | 70539-42-3 |
| MF: | C18H20N2O12 |
| MW: | 456.36 |
| EINECS: | |
| Product Categories: | homoXlink;Homobifunctional Crosslinker |
| Mol File: | 70539-42-3.mol |
|
EGS Crosslinker Chemical Properties
| Boiling point | 603.4±65.0 °C(Predicted) |
| density | 1.52±0.1 g/cm3(Predicted) |
| storage temp. | 2-8°C |
| solubility | acetic acid: water (1:1): ≤50mg/mL |
| form | powder |
| color | White to off-white |
| BRN | 9167119 |
| Major Application | diagnostic assay manufacturing
hematology
histology |
| InChI | InChI=1S/C18H20N2O12/c21-11-1-2-12(22)19(11)31-17(27)7-5-15(25)29-9-10-30-16(26)6-8-18(28)32-20-13(23)3-4-14(20)24/h1-10H2 |
| InChIKey | QLHLYJHNOCILIT-UHFFFAOYSA-N |
| SMILES | O(N1C(CCC1=O)=O)C(=O)CCC(=O)OCCOC(=O)CCC(=O)ON1C(CCC1=O)=O |
Safety Information
| WGK Germany | 3 |
| F | 10 |
| HS Code | 29280000 |
| Storage Class | 11 - Combustible Solids |
EGS Crosslinker Usage And Synthesis
| Description | EGS Crosslinker is a cleavable crosslinker which can be cleaved using hydroxyamine. EGS Crosslinkers contain two NHS ester groups which can react with primary amines. |
| Uses | Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is commonly used as a crosslinker in chemical reactions. It is a homobifunctional crosslinking reagent that is reactive with bromoacetate amines and sulfhydryls. |
| reaction suitability | reagent type: cross-linking reagent |
| Biochem/physiol Actions | Ethylene glycol-bis(succinic acid N-hydroxysuccinimide ester) is a homobifunctional, cleavable cross-linking reagent. It is mainly coupled to molecules containing primary amines by amide bonds buffered at pH 7.5 (6.5-8.5). The linkage provides a 12-atom spacer. |
| Properties and Applications | EGS crosslinker, Ethylene glycolbis(succinimidyl succinate), is a water-insoluble homobifunctional crosslinker that must be dissolved in DMSO or DMF before addition to an aqueous reaction. EGS crosslinking takes place through amine-reactive NHS esters at both ends of a 16.1-angstrom spacer arm. The 12-atom spacer arm of the EGS crosslinking reagent is cleavable and the spacer arm is cleaved by hydroxylamine at pH 8.5 for 3-6 hours at 37°C. The EGS protein crosslinker possesses amino reactive NHS esters on both ends of the molecule and can be used to label intracellular proteins since the molecule is cell membrane permeable. EGS crosslinker is often used in ChIP assays to determine DNA-protein binding interactions. The directly water-soluble analog of EGS is Sulfo-EGS. |
EGS Crosslinker Preparation Products And Raw materials
