Hygromycin B CAS 31282-04-9
Introduction：Basic information about Hygromycin B CAS 31282-04-9, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
Hygromycin B Basic information
| Product Name: | Hygromycin B |
| Synonyms: | antihelmycin;hydromycinb;hygromix2.4;hygromix-8;hygrovetine;Hygromycin Solution;Hygromix;5-O-(2,3-O-(6-Amino-6-desoxyheptapyranosyliden)-beta-D-talopyranosyl)-desoxy-N'-methyl-D-streptamin |
| CAS: | 31282-04-9 |
| MF: | C20H37N3O13 |
| MW: | 527.52 |
| EINECS: | 250-545-5 |
| Product Categories: | antibiotic;Inhibitors |
| Mol File: | 31282-04-9.mol |
Hygromycin B Chemical Properties
| Melting point | 160-180°C |
| alpha | D20 +20.2° (c = 1 in water) |
| Boiling point | 608.13°C (rough estimate) |
| density | 1.4252 (rough estimate) |
| refractive index | 1.6300 (estimate) |
| storage temp. | 2-8°C |
| solubility | H2O: 50 mg/mL As a stock solution. Stock solutions should be stored at 2-8°C. Stable at 37°C for 30 days. |
| form | powder |
| pka | 7.1, 8.8(at 25℃) |
| color | faintly brown to brown |
| PH | pH (25g/l, 25℃ ) : 9.5～12.5 |
| biological source | Streptomyces hygroscopicus |
| Water Solubility | Miscible with water, methanol, buffer solution and ethanol. Immiscible with less polar solvents. |
| Merck | 14,4852 |
| BRN | 6755837 |
| Stability: | Hygroscopic |
| InChIKey | GRRNUXAQVGOGFE-KPBUCVLVSA-N |
| EPA Substance Registry System | Hygromycin B (31282-04-9) |
Safety Information
| Hazard Codes | T+,Xn,T |
| Risk Statements | 26/27/28-41-42/43-40-36-23/24/25 |
| Safety Statements | 23-26-28-36/37/39-45-22-36-36/37 |
| RIDADR | UN 3462 6.1/PG 1 |
| WGK Germany | 3 |
| RTECS | WK2130000 |
| F | 19 |
| HazardClass | 6.1 |
| PackingGroup | I |
| HS Code | 29419059 |
| Storage Class | 6.1B - Non-combustible acute toxic Cat. 1 and 2 very toxic hazardous materials |
| Hazard Classifications | Acute Tox. 1 Inhalation Acute Tox. 3 Oral Acute Tox. 4 Dermal |
| Toxicity | mmo-esc 250 mg/L AACHAX 18,798,80 |
| Description | Hygromycin B is an aminoglycoside antibiotic that has been found in S. hygroscopicus. It is active against E. coli (MIC = 150 μg/ml) and cytotoxic to both prokaryotic and eukaryotic cells via inhibition of protein synthesis. Hygromycin B has commonly been used in molecular and cell biology applications to select for transformed cells expressing the E. coli hygromycin resistance genes hyg or hph. |
| Chemical Properties | White to off-white lyophilized powder |
| Uses | antibacterial, anthelmintic; LD50 ip (rat) 63mg/Kg |
| Uses | Hygromycin B, a selective antibiotic that is effective on most bacteria, fungi and higher eukaryotes, inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome |
| Uses | Hygromycin B is an aminoglycoside and a selective antibiotic agent for the hph gene. Hygromycin B inhibits protein synthesis by interfering with translocation and causing mistranslation at the 70S ribosome |
| Uses | Hygromycin B is a trisaccharide aminoglycoside with a terminal cyclitol produced by Streptomyces hygroscopicus, reported by researchers at Eli Lily in the early 1950s. Hygromycin B belongs to the destomycin family of aminoglycosides which contain a unique cyclic structure linking the terminal saccharide. Hygromycin B exhibits broad spectrum antibiotic and anthelmintic activity, and is used as a feed additive for swine and poultry. Hygromycin B inhibits polypeptide synthesis by binding peptidyl-tRNA and preventing translocation by Elongation Factor 2. |
| Application | Hygromycin B was found in the culture broth of Streptomyces hygroscopicus by Lilly Research Laboratories in 1953, as a mixture with hygromycin A. It showed activity against a variety of gram-positive and gram-negative bacteria as well as fungi. Hygromycin B has been used for therapy of helminthic infections in swine and poultry. |
| General Description | Amorphous solid or tan powder. |
| Air & Water Reactions | Water soluble. |
| Reactivity Profile | Weakly basic. |
| Fire Hazard | Flash point data for Hygromycin B are not available; however Hygromycin B is probably combustible. |
| Biochem/physiol Actions | Mode of Action: The product acts by inhibiting protein synthesis by inducing the misreading of the m-RNA template in the prokaryote, with the potency to inhibit translation. Antimicrobial Spectrum: Hygromycin B acts against bacteria, fungi and higher eukaryotic cells. |
| Safety Profile | Poison byintraperitoneal route. Mutation datareported. When heated to decomposition itemits toxic fumes of Nox |
| storage | +4°C |
| references | [1] brodersen de, clemons wm jr, carter ap, et al. the structural basis for the action of the antibiotics tetracycline, pactamycin, and hygromycin b on the 30s ribosomal subunit. cell, 2000, 103 (7): 1143-1154. [2] ganoza mc, kiel mc. a ribosomal atpase is a target for hygromycin b inhibition on escherichia coli ribosomes. antimicrob agents chemother, 2001, 45 (10): 2813-2819. |
Hygromycin B Preparation Products And Raw materials
| Raw materials | Streptomyceshygroscopicus |
