LUCIFERASE CAS 61970-00-1
Introduction：Basic information about LUCIFERASE CAS 61970-00-1, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
LUCIFERASE Basic informationDescription Source Background
| Product Name: | LUCIFERASE |
| Synonyms: | ALKANAL-REDUCED FMN-OXYGEN OXIDOREDUCTASE;Luciferase Photinus pyralis (firefly);Luciferase, Firefly, Recomb.;Luciferase (firefly luciferin);LUCIFERASE, RECOMBINANT, FROM LUCIOLA MINGRELICA;LUCIFERASE FROM FIREFLY, WHITE POWDER, SOLUBLE*;LUCIFERASE, FROM FIREFLY WITH*TRIS-ASPAR TIC ACID;LUCIFERASE FROM FIREFLY SOLUBLE*WITH TRI S-SUCCINIC |
| CAS: | 61970-00-1 |
| MF: | |
| MW: | 0 |
| EINECS: | 263-359-4 |
| Product Categories: | enzyme;Chemiluminescent compoundsAnalytical Enzymes;Purified EnzymesEnzyme Class Index;1.13.x.x Acting on single donors with incorporation of molecular oxygen;1.x.x.x Oxidoreductases;Biochemicals and Reagents;Luciferase;Luminescent Compounds/Detection |
| Mol File: | Mol File |
LUCIFERASE Chemical Properties
| Fp | 111℃ |
| storage temp. | -20°C |
| form | powder |
| color | white |
| Specific Activity | 15-30×106light units/mg protein |
| Major Application | diagnostic assay manufacturing |
Safety Information
| Hazard Codes | Xi,Xn,T |
| Risk Statements | 36/37/38-22-52/53-45 |
| Safety Statements | 26-36/37-24/25-23-22-61-45-53 |
| WGK Germany | 3 |
| F | 3-10-21 |
| Storage Class | 11 - Combustible Solids |
| Hazard Classifications | Resp. Sens. 1 |
| Description | Luciferase (firefly recombinant) is a light-generating enzyme that catalyzes an oxidative reaction, using luciferin, ATP, and molecular oxygen to produce oxyluciferin, which yields visible light. The bioluminescence of this ATP-dependent luciferase is commonly used in reporter systems that are amenable to high-throughput screening. |
| Uses | Firefly luciferase is used extensively in molecular and cell biology, in particular for the efficient detection and quantitation of ATP and as a reporter for genetic function. |
| Uses | Luciferase from Photinus pyralis (firefly) has been used as a component of lysis solution to measure luminescence signals, as part of the study to determine chemical signals which can activate the karrikin insensitive 2 (KAI2) pathway. |
| General Description | Firefly luciferase catalyzes the reaction of luciferin with ATP and leads to the production of yellow-green light. The enzyme has a molecular weight of 62kDa and is expressed in peroxisomes. Luciferase is considered as a model to study protein–anesthetic interactions. Firefly luciferase is highly useful in cell biology and molecular biology, as a reporter of gene function and for the quantification of ATP. |
| Biochem/physiol Actions | Firefly luciferase is a 62 kDa protein that catalyzes the production of light. The enzyme requires ATP, molecular oxygen, and the heterocyclic compound, firefly luciferin, to generate light in a two-step process. The light producing reaction is initiated by luciferin activation (adenylation of its carboxylate group) and proceeds in the presence of molecular oxygen to yield a photon of yellow-green light. |
| Description | Luciferase produced in E.Coli is a single, non-glycosylated polypeptide chain containing 571 amino acids (1-550 a.a.) and having a molecular mass of 62.9kDa. Luciferase is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Source | Escherichia Coli |
| Background | Luciferase is a general term for the class of oxidative enzymes used in bioluminescence and is distinct from a photoprotein. Luciferase catalyzes a bioluminescent reaction which involves the substrate luciferin as well as Mg2+ and ATP, produces green light with a wavelength of 562 nm. Luciferase from firefly is broadly used as a reporter for studying gene regulation and function, and for pharmaceutical screening. |
| References | [1] BALDWIN T O. Firefly luciferase: the structure is known, but the mystery remains.[J]. Structure, 1996, 4 3: 223-228. DOI: 10.1016/s0969-2126(96)00026-3 [2] TINGTING XU. The Expanding Toolbox of In Vivo Bioluminescent Imaging.[J]. Frontiers in Oncology, 2016: 150. DOI: 10.3389/fonc.2016.00150 [3] De Souza, F.I., Zumiotti, A.V., and Da Silva, C.F. Neuregulins 1-α and 1-β on the regeneration the peripheral nerves[J]. Acta Ortop Bras. |
LUCIFERASE Preparation Products And Raw materials
