PLASMIN CAS 9001-90-5
Introduction：Basic information about PLASMIN CAS 9001-90-5, including its chemical name, molecular formula, synonyms, physicochemical properties, and safety information, etc.
PLASMIN Basic information
| Product Name: | PLASMIN |
| Synonyms: | plasmin from bovine plasma;plasmin from porcine blood;PLASMIN FROM HUMAN PLASMA LYOPH. APPRO;EC 3.4.21.7;FIBRINOLYSIN;HUMAN PLASMIN;PLASMIN;PLASMIN, EACA- AND LYSINE-FREE, HUMAN PLASMA |
| CAS: | 9001-90-5 |
| MF: | |
| MW: | 0 |
| EINECS: | 232-640-3 |
| Product Categories: | |
| Mol File: | Mol File |
PLASMIN Chemical Properties
| storage temp. | -20°C |
| form | lyophilized powder |
| color | White to off-white |
| biological source | human plasma |
| Water Solubility | water: soluble |
| Merck | 13,7603 |
| Specific Activity | ≥7units/mg protein |
| Major Application | diagnostic assay manufacturing |
| EPA Substance Registry System | Plasmin (9001-90-5) |
Safety Information
| Hazard Codes | B,Xn |
| Risk Statements | 36/37/38-42 |
| Safety Statements | 2-22-24-26-36/37-45-24/25 |
| WGK Germany | 3 |
| F | 10-21 |
| TSCA | TSCA listed |
| Storage Class | 11 - Combustible Solids |
| Hazardous Substances Data | 9001-90-5(Hazardous Substances Data) |
| Originator | Actase,Ortho,US,1959 |
| Uses | Plasmin is present in blood to prevent unwanted clotting by catalysing the breakdown of the fibrin polymer that provides the framework of a blood clot. Plasmin is formed from plasminogen, a process that occurs after plasminogen has been activated by forming a complex with fibrin. After certain tissue fragments had been shown to possess plasminogenactivating ability, a soluble fraction possessing this activity was extracted and purified. Cloning and expression of complementary DNA (cDNA) for human tissue-type plasminogen activator permitted commercial production of alteplase. Alteplase was shown to be a potent thrombolytic agent by virtue of its ability to activate plasminogen, thus breaking down fibrin in the thrombus. An early clinical study in patients with coronary occlusion following myocardial infarction showed that it was an effective drug for dissolving clots in coronary arteries. It was marketed for this purpose in 1988. |
| Uses | Human plasmin has been used as an oligopeptide substrate in peptidase inhibition assays. |
| Uses | A complex between plasmin and an inhibitor has been isolated in a study via affinity chromatography from urokinase-activated human plasma. It has also been used in a study to investigate activation of human epithelial sodium channel (ENaC) by plasmin and chymotrypsin. |
| Definition | A proteolytic enzyme that dissolves fibrin and hastens the solution of clots that may form in the bloodstream. It is prepared by activating a fraction of normal human plasma with highly purified streptokinase. |
| Manufacturing Process | A 5 gallon drum of frozen plasma oxalated with a known anticoagulantquantity and proportion of oxalic acid and sodium oxalate as described in USPatent 2,394,566 is permitted to stand at room temperature (24° to 26°C) for24 hours after which the remaining unmelted portion is broken up with an icepick and a stainless steel warming coil containing running warm water atabout 40°C is inserted into the mixture and the mixture stirred. Theremaining frozen material is rapidly melted. The warming is then continuedwith vigorous agitation. When the temperature of the plasma reaches about 5° to 8°C, the calculatedquantity of calcium chloride solution is added in amount which is from 0.2 to0.3% in excess of that needed to react with and precipitate the anticoagulant.The temperature of the plasma is allowed to rise to about 24°C. At 18° to24°C strands of fibrin begin to appear and the vigor of stirring is increased toprevent a gel of fibrin from forming. Stirring is continued or 30 minutes afterthe fibrin is whipped out to allow for complete conversion of all prothrombin tothrombin and for the antithrombin to completely destroy all thrombin. At theend of this time the stirring is stopped, the fibrin allowed to rise to the surfaceand the clear serum siphoned off. If, through failure to stir with enough vigor, a gel forms instead of strands offibrin, when the temperature reaches about 18°C, the serum can also beobtained from the fibrin by working and kneading the gel in a cheesecloth bagwhile draining off the clear serum. However, this method is time-consumingand it is preferred to prevent gel formation by very vigorous stirring of themixture. The clear serum of this example is an amber liquid free from prothrombin,thrombin, fibrinogen and fibrin. It contains profibrinolysin and is excellentlysuited to further purification by salt precipitation fractionation, as given below. The special serum is brought to a temperature of about 4° to 6°C (preferably5°C) and saturated ammonium sulfate solution added drop by drop withconstant stirring to about 24 to 26% of saturation (preferably 25%). Theprecipitated protein impurities are then centrifuged off and the supernatantbrought to about -1° to +1°C (preferably 0°C). The degree of its saturation isthen brought to about 28 to 31% of saturation (preferably 29%) by furtheraddition of ammonium sulfate solution with stirring. This further degree ofsaturation precipitates the profibrinolysin which is collected by centrifugationand separated from soluble impurities. By washing the profibrinolysin severaltimes with ammonium sulfate solution of a strength which is 29% ofsaturation a practically white solid is obtained which can be freeze-dried(frozen and dried under reduced pressure) to give a dry, white, productcontaining purified profibrinolysin free from thromboplastin, prothrombin,thrombin, fibrinogen and fibrin, (from US Patent 2,624,691), which is thenactivated to fibrinolysin. |
| Therapeutic Function | Thrombolytic |
| General Description | Fibrinolysin |
| Biochem/physiol Actions | Plasmin is a direct acting fibrinolytic enzyme present in blood. Its activity is independent of plasminogen presence during fibrinolysis. Plasmin is rapidly and irreversibly inhibited by α-2 antiplasmin. |
PLASMIN Preparation Products And Raw materials
| Raw materials | Ammonium sulfate-->Calcium chloride-->Oxalic acid |
